| 1. |
- Adam, A, et al.
(författare)
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Abstracts from Hydrocephalus 2016.
- 2017
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Ingår i: Fluids and Barriers of the CNS. - : Springer Science and Business Media LLC. - 2045-8118. ; 14:Suppl 1
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Tidskriftsartikel (refereegranskat)
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| 2. |
- Nilsson, C. L., et al.
(författare)
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Chromosome 19 Annotations with Disease Speciation: A First Report from the Global Research Consortium
- 2013
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 12:1, s. 134-149
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Tidskriftsartikel (refereegranskat)abstract
- A first research development progress report of the Chromosome 19 Consortium with members from Sweden, Norway, Spain, United States, China and India, a part of the Chromosome-centric Human Proteome Project (C-HPP) global initiative, is presented (http://www.c-hpp.org). From the chromosome 19 peptide-targeted library constituting 6159 peptides, a pilot study was conducted using a subset with 125 isotope-labeled peptides. We applied an annotation strategy with triple quadrupole, ESI-Qtrap, and MALDI mass spectrometry platforms, comparing the quality of data within and in between these instrumental set-ups. LC–MS conditions were outlined by multiplex assay developments, followed by MRM assay developments. SRM was applied to biobank samples, quantifying kallikrein 3 (prostate specific antigen) in plasma from prostate cancer patients. The antibody production has been initiated for more than 1200 genes from the entire chromosome 19, and the progress developments are presented. We developed a dedicated transcript microarray to serve as the mRNA identifier by screening cancer cell lines. NAPPA protein arrays were built to align with the transcript data with the Chromosome 19 NAPPA chip, dedicated to 90 proteins, as the first development delivery. We have introduced an IT-infrastructure utilizing a LIMS system that serves as the key interface for the research teams to share and explore data generated within the project. The cross-site data repository will form the basis for sample processing, including biological samples as well as patient samples from national Biobanks.
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| 3. |
- Adlanmerini, M., et al.
(författare)
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Mutation of Arginine 264 on ER alpha (Estrogen Receptor Alpha) Selectively Abrogates the Rapid Signaling of Estradiol in the Endothelium Without Altering Fertility
- 2020
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Ingår i: Arteriosclerosis, Thrombosis, and Vascular Biology. - : Ovid Technologies (Wolters Kluwer Health). - 1079-5642 .- 1524-4636. ; 40:9, s. 2143-2158
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Tidskriftsartikel (refereegranskat)abstract
- Objective: ER alpha (estrogen receptor alpha) exerts nuclear genomic actions and also rapid membrane-initiated steroid signaling. The mutation of the cysteine 451 into alanine in vivo has recently revealed the key role of this ER alpha palmitoylation site on some vasculoprotective actions of 17 beta-estradiol (E2) and fertility. Here, we studied the in vivo role of the arginine 260 of ER alpha which has also been described to be involved in its E2-induced rapid signaling with PI-3K (phosphoinositide 3-kinase) as well as G protein in cultured cell lines. Approach and Results: We generated a mouse model harboring a point mutation of the murine counterpart of this arginine into alanine (R264A-ER alpha). In contrast to theC451A-ER alpha, theR264A-ER alpha females are fertile with standard hormonal serum levels and normal control of hypothalamus-pituitary ovarian axis. Although R264A-ER alpha protein abundance was normal, the well-described membrane ER alpha-dependent actions of estradiol, such as the rapid dilation of mesenteric arteries and the acceleration of endothelial repair of carotid, were abrogated inR264A-ER alpha mice. In striking contrast, E2-regulated gene expression was highly preserved in the uterus and the aorta, revealing intact nuclear/genomic actions in response to E2. Consistently, 2 recognized nuclear ER alpha-dependent actions of E2, namely atheroma prevention and flow-mediated arterial remodeling were totally preserved. Conclusions: These data underline the exquisite role of arginine 264 of ER alpha for endothelial membrane-initiated steroid signaling effects of E2 but not for nuclear/genomic actions. This provides the first model of fertile mouse with no overt endocrine abnormalities with specific loss-of-function of rapid ER alpha signaling in vascular functions.
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| 4. |
- Rotermund, F., et al.
(författare)
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Efficient femtosecond travelling-wave parametric amplification in periodically poled KTiOPO4
- 1999
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Ingår i: Optics Letters. - : Optical Society of America. - 0146-9592 .- 1539-4794. ; 24, s. 1874-1876
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Tidskriftsartikel (refereegranskat)abstract
- We report, for the first time to our knowledge, high-power femtosecond traveling-wave optical parametric amplification by use of periodically poled KTiOPO4. With a single pass through a 4-mm-long sample of 1.23-mm thickness we achieved 40% internal conversion efficiency and 5 μJ of single-pulse idler energy near 3.8 μm, using only 75 μJ of energy from the output of a conventional 1-kHz Ti:sapphire regenerative amplifier. The 210-fs-long idler pulses were almost transform limited. We discuss the specific problems encountered in high-power parametric conversion, such as unwanted quasi-phase-matched upconversion processes for polarization configurations that utilize the largest (d33) nonlinear coefficient and the related formation of color centers (gray tracking) in KTiOPO4.
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| 5. |
- Bengtsson, Martin, et al.
(författare)
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Influence of Nonuniform Channel Width Distribution in Porous Silicon High Aspect Ratio Parallel Channel Micro Reactors
- 2005
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Ingår i: [Host publication title missing]. - 0854046437 ; 1, s. 629-631
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Konferensbidrag (refereegranskat)abstract
- The paper focuses on the fact that, since the flow rate in parallel channels relies strongly on the channel width, the combination may lead to inaccurate results if errors in the fabrication process lead to an uneven distribution of channel widths. Parallel channel enzyme reactors were designed with channel widths distributed normally with different degrees of standard deviation. The reactors were then evaluated with regard to dispersion and to catalytic effect of the immobilised enzyme. It was shown that for lower concentrations the catalytic efficiency decreased significantly even for small variations in the distribution of channel widths and reactors with poor homogeneity in channel widths also diluted the sample more than the others.
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