| 1. |
- Lawrenson, Kate, et al.
(författare)
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Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus
- 2016
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Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 7
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Tidskriftsartikel (refereegranskat)abstract
- A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P=9.2 × 10-20), ER-negative BC (P=1.1 × 10-13), BRCA1-associated BC (P=7.7 × 10-16) and triple negative BC (P-diff=2 × 10-5). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P=2 × 10-3) and ABHD8 (P<2 × 10-3). Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter. Targeted deletion of a region containing risk SNP rs56069439 in a putative enhancer induces ANKLE1 downregulation; and mRNA stability assays indicate functional effects for an ANKLE1 3′-UTR SNP. Altogether, these data suggest that multiple SNPs at 19p13 regulate ABHD8 and perhaps ANKLE1 expression, and indicate common mechanisms underlying breast and ovarian cancer risk.
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| 2. |
- ELLIOTT, MARINA, et al.
(författare)
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Expanded Explorations of the Dinaledi Subsystem,Rising Star Cave System, South Africa
- 2021
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Ingår i: PaleoAnthropology. - 1545-0031. ; 2021:1, s. 15-22
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Tidskriftsartikel (refereegranskat)abstract
- The Dinaledi Chamber of the Rising Star cave system has yielded a large assemblage of fossil hominin material, attributed to Homo naledi. The unusual taphonomic and geological situation of the assemblage suggested that the remains may have been deliberately deposited in the chamber. However, the route and mechanism of deposition of the remains within the Dinaledi Chamber are still uncertain. During the 2017—2018 field seasons, we expanded explorations of the passages surrounding the Dinaledi Chamber. These explorations improved our understandingof the cave’s spatial complexity, necessitating a revision of the way the spaces are named and described. The work supported the hypothesis that there is no alternate entrance into the system other than the Chute. The work also identified new fossil deposits in several remote passages, three of which contain material attributable to H. naledi. Here, we clarify the definition of the Dinaledi Subsystem and provide terminology for new fossil localities found in this portion of the Rising Star cave system. These results emphasize the complex depositional environment of the Dinaledi Subsystem and raise new questions about the process and timing of the fossil accumulations.
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| 3. |
- Jacobson, Ken, et al.
(författare)
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Lipid flow in locomoting cells : Response
- 1991
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Ingår i: Science. - : American Association for the Advancement of Science. - 0036-8075 .- 1095-9203. ; 251:4991, s. 318-318
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Tidskriftsartikel (refereegranskat)abstract
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| 4. |
- Lee, Juliet, et al.
(författare)
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The direction of membrane lipid flow in locomoting polymorphonuclear leukocytes
- 1990
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Ingår i: Science. - : American Association for the Advancement of Science. - 0036-8075 .- 1095-9203. ; 247:4947, s. 1229-1233
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Tidskriftsartikel (refereegranskat)abstract
- The objective of this study was to determine the direction of membrane lipid flow in locomoting cells. The plasma membrane of human polymorphonuclear leukocytes was stained with a fluorescent lipid analog dihexadecanoyl indocarbocyanine. A line was photobleached on the cell surface perpendicular to the direction of cell motion. Low-light-level fluorescence microscopy and digital image-processing techniques were used to analyze a series of images taken at short intervals after photobleaching. The bleached line remained visible for about 5 seconds before being erased by diffusional recovery. Examination of fluorescence intensity profiles allowed a comparison to be made between the velocities of line and cell movement. Results indicate that the bleached line moves forward with the same velocity as the cell during locomotion, refuting the retrograde lipid flow model of locomotion. Instead, the plasma membrane lipid appears to move forward according to either the unit movement of membrane or the tank track model of locomotion.
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