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- Magnusson, Cecilia, et al.
(författare)
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Acoustic Enrichment of Heterogeneous Circulating Tumor Cells and Clusters from Metastatic Prostate Cancer Patients
- 2024
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 1520-6882 .- 0003-2700. ; 96:18, s. 6914-6921
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: There are important unmet clinical needs to develop cell enrichment technologies to enable unbiased label-free isolation of both single cell and clusters of circulating tumor cells (CTCs) manifesting heterogeneous lineage specificity. Here, we report a pilot study based on the microfluidic acoustophoresis enrichment of CTCs using the CellSearch CTC assay as a reference modality.METHODS: Acoustophoresis uses an ultrasonic standing wave field to separate cells based on biomechanical properties (size, density, and compressibility), resulting in inherently label-free and epitope-independent cell enrichment. Following red blood cell lysis and paraformaldehyde fixation, 6 mL of whole blood from 12 patients with metastatic prostate cancer and 20 healthy controls were processed with acoustophoresis and subsequent image cytometry.RESULTS: Acoustophoresis enabled enrichment and characterization of phenotypic CTCs (EpCAM +, Cytokeratin +, DAPI +, CD45 -/CD66b -) in all patients with metastatic prostate cancer and detected CTC-clusters composed of only CTCs or heterogeneous aggregates of CTCs clustered with various types of white blood cells in 9 out of 12 patients. By contrast, CellSearch did not detect any CTC clusters, but detected comparable numbers of phenotypic CTCs as acoustophoresis, with trends of finding a higher number of CTCs using acoustophoresis. CONCLUSION: Our preliminary data indicate that acoustophoresis provides excellent possibilities to detect and characterize CTC clusters as a putative marker of metastatic disease and outcomes. Moreover, acoustophoresis enables the sensitive label-free enrichment of cells with epithelial phenotypes in blood and offers opportunities to detect and characterize CTCs undergoing epithelial-to-mesenchymal transitioning and lineage plasticity.
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- Magnusson, Cecilia, et al.
(författare)
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Acoustic enrichment of heterogenous circulating tumor cells and clusters from patients with metastatic prostate cancer
- 2023
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- BACKGROUND: There are important unmet clinical needs to develop cell enrichment technologies to enable unbiased label-free isolation of both single cell and clusters of circulating tumor cells (CTCs) manifesting heterogeneous lineage specificity. Here, we report a pilot study based on microfluidic acoustophoresis enrichment of CTCs using the CellSearch CTC assay as a reference modality.METHODS: Acoustophoresis uses an ultrasonic standing wave field to separate cells based on biomechanical properties (size, density, and compressibility) resulting in inherently label-free and epitope-independent cell enrichment. Following red blood cell lysis and paraformaldehyde fixation, 6 mL of whole blood from 12 patients with metastatic prostate cancer and 20 healthy controls were processed with acoustophoresis and subsequent image cytometry.RESULTS: Acoustophoresis enabled enrichment and characterization of phenotypic CTCs (EpCAM + , Cytokeratin + , DAPI + , CD45 - /CD66b - ) in all patients with metastatic prostate cancer and detected CTC-clusters composed of only CTCs or heterogenous aggregates of CTCs clustered with various types of white blood cells in 9 out of 12 patients. By contrast, CellSearch did not detect any CTC-clusters, but detected comparable numbers of phenotypic CTCs as acoustophoresis, with trends of finding higher number of CTCs using acoustophoresis. CONCLUSION: Our preliminary data indicate that acoustophoresis provides excellent possibilities to detect and characterize CTC-clusters as a putative marker of metastatic disease and outcomes. Moreover, acoustophoresis enables sensitive label-free enrichment of cells with epithelial phenotype in blood and offers opportunities to detect and characterize CTCs undergoing epithelial-to-mesenchymal transitioning and lineage plasticity.
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- Andersson, Klara, et al.
(författare)
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ANTISYMMETRIC ACTUATION INCREASES ACOUSTOPHORESIS PERFORMANCE
- 2021
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Ingår i: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 901-902
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Konferensbidrag (refereegranskat)abstract
- Antisymmetric, symmetric and standard actuation of acoustofluidic devices were compared in terms of focusability. By imaging the end part of an acoustophoresis separation channel while flowing fluorescent beads through it, we show that beads get more tightly focused by antisymmetric actuation than symmetric and standard actuation at a given electrical input power. This means that antisymmetric actuation is more suitable for single node acoustic focusing as lower input power can be used to reach the same level of performance, or higher throughput can be reached for the same power.
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- Bjursten, Henrik, et al.
(författare)
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Particle separation using ultrasound can radically reduce embolic load to brain after cardiac surgery.
- 2004
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Ingår i: Annals of Thoracic Surgery. - : Elsevier BV. - 1552-6259 .- 0003-4975. ; 78:5, s. 1572-1578
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Tidskriftsartikel (refereegranskat)abstract
- Background. Microembolism during cardiopulmonary bypass has been suggested as being the predominant cause of neurocognitive disorders after cardiac surgery. Shed blood, normally retransfused into the patient during cardiopulmonary bypass, is a major source of lipid microemboli in the brain capillaries. A newly developed technique based on acoustic standing-wave separation of particles in fluid in microchannels, with the capacity to remove lipid particles in blood, is presented. Methods. A separator consisting of eight parallel, high-fidelity microfabricated channels was actuated with an ultrasound field to create a standing wave. Three different concentrations of lipid particles (diameter, 0.3 mum) were added to blood samples with increasing hematocrits and introduced into the separator channels to separate lipid particles and erythrocytes. Results. The mean separation rates for lipid particles were 81.9% +/- 7.6% and for erythrocytes 79.8% +/- 9.9%, and both were related to the hematocrit level of the incoming blood sample. The procedure was atraumatic and did not cause hemolysis. Conclusions. Particle separation by means of an acoustic standing-wave technique can be used for atraumatic and effective removal of lipid particles from blood, with the possible clinical implication of reducing neurocognitive complications after cardiopulmonary bypass. (C) 2004 by The Society of Thoracic Surgeons.
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- Broman, Axel, et al.
(författare)
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Multinodal Acoustic Trapping Enables High Capacity and High Throughput Enrichment of Extracellular Vesicles and Microparticles in miRNA and MS Proteomics Studies
- 2021
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 1520-6882 .- 0003-2700. ; 93:8, s. 3929-3937
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Tidskriftsartikel (refereegranskat)abstract
- We report a new design of an acoustophoretic trapping device with significantly increased capacity and throughput, compared to current commercial acoustic trapping systems. Acoustic trapping enables nanoparticle and extracellular vesicle (EV) enrichment without ultracentrifugation. Current commercial acoustic trapping technology uses an acoustic single-node resonance and typically operates at flow rates <50 μL/min, which limits the processing of the larger samples. Here, we use a larger capillary that supports an acoustic multinode resonance, which increased the seed particle capacity 40 times and throughput 25-40 times compared to single-node systems. The resulting increase in capacity and throughput was demonstrated by isolation of nanogram amounts of microRNA from acoustically trapped urinary EVs within 10 min. Additionally, the improved trapping performance enabled isolation of extracellular vesicles for downstream mass spectrometry analysis. This was demonstrated by the differential protein abundance profiling of urine samples (1-3 mL), derived from the non-trapped versus trapped urine samples.
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- Broman, Axel, et al.
(författare)
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Multinodal high throughput acoustic trapping of exosomes from urine samples
- 2019
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Ingår i: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. - 9781733419000 ; , s. 2-3
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Konferensbidrag (refereegranskat)abstract
- We report a new design of an acoustophoretic trapping device with greatly increased capacity and throughput, compared to current commercial systems. Acoustic trapping enables nanoparticle and exosome enrichment without the need for ultracentrifugation. The commercial acoustic trapping technology, AcouTrap (AcouSort AB), typically operates at flow rates < 50 μl/min which limits processing of larger samples. Using a larger capillary that supports an acoustic multi-node resonance, capacity and throughput was increased a factor 40 compared to AcouTrap. It was possible to capture and enrich exosomes from urine samples at 30 times higher flow rate than previously reported.
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