| 1. |
- Denoyes-Rothan, B., et al.
(författare)
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Anthracnose on strawberry in France : Situation and perspectives
- 2006
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Ingår i: Proceedings of the Vth International Strawberry Symposium. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 277-280
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Konferensbidrag (refereegranskat)abstract
- Anthracnose, caused by Colletotrichum acutatum, on strawberry (Fragaria x ananassa) was first reported on fruit in France in 1981. Damage was later recorded on runners, petioles, foliage and crowns. The evolution of a disease control method, the molecular variability of C acutatum, the study of the interaction between pathogen and host, the different techniques of inoculation, and the last results concerning QTLs linked to resistance to C acutatum are reported, which cover 10 years of research in France.
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| 2. |
- Denoyes-Rothan, B, et al.
(författare)
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Inheritance of resistance to Colletotrichum acutatum in Fragaria x ananassa
- 2005
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Ingår i: Phytopathology. - : AMER PHYTOPATHOLOGICAL SOC. - 0031-949X .- 1943-7684. ; 95:4, s. 405-412
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Tidskriftsartikel (refereegranskat)abstract
- Anthracnose, caused by Colletotrichum acutatum, is a major disease of the octoploid cultivated strawberry, Fragaria x ananassa. The inheritance of high and intermediate level plant resistances to C. acutatum, pathogenicity group 2, was investigated in an 8 x 8 factorial design. A single dominant gene (Rca2) controlled the high-level resistance, although minor genes may also contribute to resistance in cultivars such as Belrubi. The intermediate level of resistance was quantitative and controlled by minor genes. Analysis of 26 genotypes and cultivars from Fragaria spp. showed that the dominant gene was not rare in the germ plasm of E x ananassa and that anthracnose resistance was also present in other species of Fragaria. These findings have important implications for anthracnose resistance breeding.
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| 3. |
- Denoyes-Rothan, B, et al.
(författare)
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QTL analysis for resistances to Colletotrichum acutatum and Phytophthora cactorum in octoploid strawberry (Fragaria x ananassa)
- 2004
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Ingår i: Proceedings of the XIth Eucarpia Symposium on Fruit Breeding and Genetics, Vols 1 and 2. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 147-151
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Konferensbidrag (refereegranskat)abstract
- Colletotrichum acutatum and Phytophthora cactorum are two of the most important pathogens of strawberry in Europe, and particularly in France. The characterization of quantitative trait loci (QTL) controlling resistances to these two pathogens would allow a better knowledge of the genetic architecture of these resistances in order to develop durable resistance. A pseudo-testcross strawberry population derived from the cross between Capitola and CF1116 was used for detecting QTLs associated with C. acutatum and P cactorum resistances. One hundred and eighty five individuals were inoculated with C. acutatum by dipping cold stored plants in a conidial suspension adjusted to 2.10(6) conidia per ml. They were also inoculated separately with P cactorum by inserting an agar disk containing mycelium into the crown. Each inoculation was conducted twice. Putative QTLs for resistance to C acutatum and P cactorum were identified by Interval Mapping (Mapmaker-QTL) using a LOD threshold of 2.
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| 4. |
- Denoyes-Rothan, B., et al.
(författare)
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Use of a Reference Octoploid Map for Detecting Homoeologous QTLs Potentially Involved in Viability in the Cultivated Strawberry
- 2009
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Ingår i: VI INTERNATIONAL STRAWBERRY SYMPOSIUM. - : INT SOC HORTICULTURAL SCIENCE. ; , s. 537-540
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Konferensbidrag (refereegranskat)abstract
- The construction of a reference map of the complex octoploid cultivated strawberry was optimized by comparative mapping between Fragaria showing different levels of ploidy. In addition, this approach is a powerful tool for improving the detection of QTLs, and for a better understanding of the organization of these QTLs. By identifying the homoeologous linkage groups in the complex octoploid cultivated strawberry, potential homoeologous QTLs in Fragaria can be detected. The comparative mapping was based on two populations belonging to two different levels of Fragaria. The linkage map of the complex octoploid cultivated strawberry (Fragaria x ananassa Duch., 2n=8x=56) was based on a segregating population of 213 individuals of a cross between ‘Capitola’ and ‘CF1116’, two genotypes with many contrasting traits. This genetic map was compared to the diploid one, obtained from an interspecific cross between F. vesca and F. bucharica (Sargent et al., 2006). Based on 56 SSR markers common to both diploid and octoploid genetic maps, almost all linkage groups of the genetic octoploid map were arranged into the seven homoeologous groups (HGs) expected for the genus Fragaria, which belongs to the tribe Rosoideae. For each trait, QTLs can potentially be detected on each linkage group belonging to the same HG. Results showed detection of homoeologous chromosomal regions potentially involved in viability of the plant.
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| 5. |
- Gaston, Amelia, et al.
(författare)
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PFRU, a single dominant locus regulates the balance between sexual and asexual plant reproduction in cultivated strawberry
- 2013
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Ingår i: Journal of Experimental Botany. - : OXFORD UNIV PRESS. - 0022-0957 .- 1460-2431. ; 64:7, s. 1837-1848
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Tidskriftsartikel (refereegranskat)abstract
- Strawberry (Fragaria sp.) stands as an interesting model for studying flowering behaviour and its relationship with asexual plant reproduction in polycarpic perennial plants. Strawberry produces both inflorescences and stolons (also called runners), which are lateral stems growing at the soil surface and producing new clone plants. In this study, the flowering and runnering behaviour of two cultivated octoploid strawberry (Fragariaananassa Duch., 2n856) genotypes, a seasonal flowering genotype CF1116 and a perpetual flowering genotype Capitola, were studied along the growing season. The genetic bases of the perpetual flowering and runnering traits were investigated further using a pseudo full-sibling F1 population issued from a cross between these two genotypes. The results showed that a single major quantitative trait locus (QTL) named FaPFRU controlled both traits in the cultivated octoploid strawberry. This locus was not orthologous to the loci affecting perpetual flowering (SFL) and runnering (R) in Fragaria vesca, therefore suggesting different genetic control of perpetual flowering and runnering in the diploid and octoploid Fragaria spp. Furthermore, the FaPFRU QTL displayed opposite effects on flowering (positive effect) and on runnering (negative effect), indicating that both traits share common physiological control. These results suggest that this locus plays a major role in strawberry plant fitness by controlling the balance between sexual and asexual plant reproduction.
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| 6. |
- Lerceteau, Estelle, et al.
(författare)
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AFLP mapping and detection of quantitative trait loci (QTLs) for economically important traits in Pinus sylvestris : a preliminary study
- 2000
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Ingår i: Molecular breeding. - : KLUWER ACADEMIC PUBL. - 1380-3743 .- 1572-9788. ; 6:5, s. 451-458
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Tidskriftsartikel (refereegranskat)abstract
- We have applied a two-way pseudo-testcross strategy in an analysis of Pinus sylvestris for genetic mapping and detection of quantitative trait loci (QTLs) associated with economically important traits targeted in the Swedish tree-breeding programme. Based on 94 full-sib progeny of a cross between two plus-trees from northern Sweden we generated two parental maps using AFLP markers. The female map was comprised of 94 markers assigned to 15 linkage groups giving a size of 796 cM. On the male map 155 markers were assigned to 15 linkage groups, giving a total size of 1335 cM. The recombination frequency was found to be sex-dependent, being 29.3% higher in male than in female gametes. On the female map, 12 QTLs were detected (but none for branch diameter or wood density). Three QTLs for tree height accounted for 25.8% of the total phenotypic variation of this trait. When the QTLs detected for all the traits were taken independently, the percentages of phenotypic variance ranged from 9.3% to 22.7%. The highest value was observed for frost hardiness, an important trait in northern Sweden for which a major gene seemed to be involved. A cluster of QTLs for tree height, trunk diameter and volume was located on one linkage group. On the male map, four QTLs for trunk diameter and volume were detected. Due to the reduced number of individuals under study, the results are preliminary and have to be validated on more trees.
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| 7. |
- Lerceteau, Estelle, et al.
(författare)
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Detection of quantitative trait loci in Pinus sylvestris L. across years
- 2001
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Ingår i: Euphytica. - : KLUWER ACADEMIC PUBL. - 0014-2336 .- 1573-5060. ; 121:2, s. 117-122
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Tidskriftsartikel (refereegranskat)abstract
- In an earlier study, we reported the characterisation of quantitative trait loci (QTLs) for economically important traits related to wood production for a specific year of measurement. However, validating the detected QTLs across years is important for any strategy for marker-assisted selection (MAS). Therefore, we evaluated the consistency of the QTLs across four years in 9 to 12 year-old trees. Data related to tree height were analysed in two ways. The total height was used to characterise QTLs likely to be useful in MAS, and the increment values to evaluate growth regulation. Among the 11 QTLs detected, 4 were found in both the 1996 and 1997 evaluations. QTL numbers and locations related to total height were found to be highly stable. However, the QTL effects progressively changed in successive years, following either an increasing, decreasing or curvilinear trend. Depending on their specific trends, some QTLs may not be expressed at all later in maturity, or they may be expressed at a higher level. This will have direct consequences on the applicability of molecular markers in early screening programs, since a study at the mature stage may also be required. More variation in QTL number and effect was observed relating to height increment than to total height, indicating significant differences in gene expression during the growth periods involved.
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| 8. |
- Lerceteau, Estelle, et al.
(författare)
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Evaluation of the extent of genetic variability among Theobroma cacao accessions using RAPD and RFLP markers
- 1997
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Ingår i: Theoretical and Applied Genetics. - : SPRINGER. - 0040-5752 .- 1432-2242. ; 95:1-2, s. 10-19
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Tidskriftsartikel (refereegranskat)abstract
- Random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers were used to evaluate genetic relationships within the Theobroma cacao species and to assess the organization of its genetic diversity. Genetic variability was estimated with 18 primers and 43 RFLP probes on 155 cocoa trees belonging to different morphological groups and coming from various geographic origins. The majority of the RFLP probes issued from low-copy DNA sequences. On the basis of on the genetic distance matrices, the two molecular methods gave related estimates of the genetic relationship between genotypes. Although an influence of cocoa morphological groups and geographical origins of trees was observed, a lack of gene differentiation characterized the T. cacao accessions studied. The continuous RFLP variability observed within the species may reflect the hybridization and introgressions between trees of different origins. Nevertheless, the Nacional type was detected to be genetically specific and different from well-known types such as Forastero, Criollo and Trinitario. Some of those genotypes were characterized by a low heterozygosity rate and may constitute the original Nacional pool. These results also provide information for the constitution of a cocoa tree core collection.
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| 9. |
- Lerceteau, Estelle, et al.
(författare)
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Evolution of cacao bean proteins during fermentation : a study by two-dimensional electrophoresis
- 1999
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Ingår i: Journal of the Science of Food and Agriculture. - : JOHN WILEY & SONS LTD. - 0022-5142 .- 1097-0010. ; 79:4, s. 619-625
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Tidskriftsartikel (refereegranskat)abstract
- The protein content of cacao (Theobroma cacao) beans was studied by quantitative two-dimensional electrophoresis (2-DE) and by measuring total and protein nitrogen by the Kjeldahl method from the unfermented stage up to the seventh day of fermentation. The major trends in evolution of protein concentration were followed by measuring the intensities of some of the most abundant bean polypeptides. During fermentation a biphasic proteolytic process was observed. Protein degradation was detected after two days of fermentation, and was most pronounced during the third day. Following the initial phase of degradation until the end of fermentation, very little further protein degradation was observed, possibly due to the release of polyphenolic compounds and their subsequent complexing with the remaining proteins. Total protein estimated by the Kjeldahl method decreased to 57% of the initial value during the fermentation period. The process of degradation is selective, with some polypeptides resisting more than others. The evolution of total protein and non-protein nitrogen content is also described. (C) 1999 Society of Chemical Industry.
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| 10. |
- Lerceteau, Estelle, et al.
(författare)
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Genetic differentiation among Ecuadorian Theobroma cacao L accessions using DNA and morphological analyses
- 1997
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Ingår i: Euphytica. - : SPRINGER. - 0014-2336 .- 1573-5060. ; 95:1, s. 77-87
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Tidskriftsartikel (refereegranskat)abstract
- The quality of Ecuadorian cacao is presently threatened by the introduction of hybrid material. An estimation of genetic diversity in Ecuador is required in order to avoid the loss of fine flavored cocoa. Genetic variability amongst 60 Ecuadorian genotypes of Theobroma cacao has been evaluated using molecular and phenotypic markers. The two distance matrices derived from the molecular and phenotypic data were found to be correlated (R-2 = 0.5). Dynamic clustering analyses classified the genotypes in two or three groups depending on the markers used. The genotypes coming from Sebastian Arteaga (SA) and Balao Chico (BCH) plantations appeared related to each other suggesting a common genetic origin. They also may be considered as a distinct group with high RFLP homozygosity. The EETP (Estacion Experimental Tropical Pichilingue of Ecuador) collection was comprised of more variable genotypes possessing variable heterozygosity levels. The low heterozygous genotypes may be genetically related to SA and BCH trees, whereas the higher heterozygous genotypes may have resulted from hybridizations between original Nacional material of Ecuador and genotypes imported from Trinidad at the beginning of the century. Thus genetic introgression may have occurred giving rise to a range of variation between Nacional and hybrid forms.
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