| 1. |
- Denoyes-Rothan, B., et al.
(författare)
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Anthracnose on strawberry in France : Situation and perspectives
- 2006
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Ingår i: Proceedings of the Vth International Strawberry Symposium. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 277-280
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Konferensbidrag (refereegranskat)abstract
- Anthracnose, caused by Colletotrichum acutatum, on strawberry (Fragaria x ananassa) was first reported on fruit in France in 1981. Damage was later recorded on runners, petioles, foliage and crowns. The evolution of a disease control method, the molecular variability of C acutatum, the study of the interaction between pathogen and host, the different techniques of inoculation, and the last results concerning QTLs linked to resistance to C acutatum are reported, which cover 10 years of research in France.
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| 2. |
- Denoyes-Rothan, B, et al.
(författare)
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Inheritance of resistance to Colletotrichum acutatum in Fragaria x ananassa
- 2005
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Ingår i: Phytopathology. - : AMER PHYTOPATHOLOGICAL SOC. - 0031-949X .- 1943-7684. ; 95:4, s. 405-412
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Tidskriftsartikel (refereegranskat)abstract
- Anthracnose, caused by Colletotrichum acutatum, is a major disease of the octoploid cultivated strawberry, Fragaria x ananassa. The inheritance of high and intermediate level plant resistances to C. acutatum, pathogenicity group 2, was investigated in an 8 x 8 factorial design. A single dominant gene (Rca2) controlled the high-level resistance, although minor genes may also contribute to resistance in cultivars such as Belrubi. The intermediate level of resistance was quantitative and controlled by minor genes. Analysis of 26 genotypes and cultivars from Fragaria spp. showed that the dominant gene was not rare in the germ plasm of E x ananassa and that anthracnose resistance was also present in other species of Fragaria. These findings have important implications for anthracnose resistance breeding.
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| 3. |
- Denoyes-Rothan, B, et al.
(författare)
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QTL analysis for resistances to Colletotrichum acutatum and Phytophthora cactorum in octoploid strawberry (Fragaria x ananassa)
- 2004
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Ingår i: Proceedings of the XIth Eucarpia Symposium on Fruit Breeding and Genetics, Vols 1 and 2. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 147-151
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Konferensbidrag (refereegranskat)abstract
- Colletotrichum acutatum and Phytophthora cactorum are two of the most important pathogens of strawberry in Europe, and particularly in France. The characterization of quantitative trait loci (QTL) controlling resistances to these two pathogens would allow a better knowledge of the genetic architecture of these resistances in order to develop durable resistance. A pseudo-testcross strawberry population derived from the cross between Capitola and CF1116 was used for detecting QTLs associated with C. acutatum and P cactorum resistances. One hundred and eighty five individuals were inoculated with C. acutatum by dipping cold stored plants in a conidial suspension adjusted to 2.10(6) conidia per ml. They were also inoculated separately with P cactorum by inserting an agar disk containing mycelium into the crown. Each inoculation was conducted twice. Putative QTLs for resistance to C acutatum and P cactorum were identified by Interval Mapping (Mapmaker-QTL) using a LOD threshold of 2.
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| 4. |
- Denoyes-Rothan, B., et al.
(författare)
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Use of a Reference Octoploid Map for Detecting Homoeologous QTLs Potentially Involved in Viability in the Cultivated Strawberry
- 2009
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Ingår i: VI INTERNATIONAL STRAWBERRY SYMPOSIUM. - : INT SOC HORTICULTURAL SCIENCE. ; , s. 537-540
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Konferensbidrag (refereegranskat)abstract
- The construction of a reference map of the complex octoploid cultivated strawberry was optimized by comparative mapping between Fragaria showing different levels of ploidy. In addition, this approach is a powerful tool for improving the detection of QTLs, and for a better understanding of the organization of these QTLs. By identifying the homoeologous linkage groups in the complex octoploid cultivated strawberry, potential homoeologous QTLs in Fragaria can be detected. The comparative mapping was based on two populations belonging to two different levels of Fragaria. The linkage map of the complex octoploid cultivated strawberry (Fragaria x ananassa Duch., 2n=8x=56) was based on a segregating population of 213 individuals of a cross between ‘Capitola’ and ‘CF1116’, two genotypes with many contrasting traits. This genetic map was compared to the diploid one, obtained from an interspecific cross between F. vesca and F. bucharica (Sargent et al., 2006). Based on 56 SSR markers common to both diploid and octoploid genetic maps, almost all linkage groups of the genetic octoploid map were arranged into the seven homoeologous groups (HGs) expected for the genus Fragaria, which belongs to the tribe Rosoideae. For each trait, QTLs can potentially be detected on each linkage group belonging to the same HG. Results showed detection of homoeologous chromosomal regions potentially involved in viability of the plant.
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| 5. |
- Gaston, Amelia, et al.
(författare)
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PFRU, a single dominant locus regulates the balance between sexual and asexual plant reproduction in cultivated strawberry
- 2013
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Ingår i: Journal of Experimental Botany. - : OXFORD UNIV PRESS. - 0022-0957 .- 1460-2431. ; 64:7, s. 1837-1848
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Tidskriftsartikel (refereegranskat)abstract
- Strawberry (Fragaria sp.) stands as an interesting model for studying flowering behaviour and its relationship with asexual plant reproduction in polycarpic perennial plants. Strawberry produces both inflorescences and stolons (also called runners), which are lateral stems growing at the soil surface and producing new clone plants. In this study, the flowering and runnering behaviour of two cultivated octoploid strawberry (Fragariaananassa Duch., 2n856) genotypes, a seasonal flowering genotype CF1116 and a perpetual flowering genotype Capitola, were studied along the growing season. The genetic bases of the perpetual flowering and runnering traits were investigated further using a pseudo full-sibling F1 population issued from a cross between these two genotypes. The results showed that a single major quantitative trait locus (QTL) named FaPFRU controlled both traits in the cultivated octoploid strawberry. This locus was not orthologous to the loci affecting perpetual flowering (SFL) and runnering (R) in Fragaria vesca, therefore suggesting different genetic control of perpetual flowering and runnering in the diploid and octoploid Fragaria spp. Furthermore, the FaPFRU QTL displayed opposite effects on flowering (positive effect) and on runnering (negative effect), indicating that both traits share common physiological control. These results suggest that this locus plays a major role in strawberry plant fitness by controlling the balance between sexual and asexual plant reproduction.
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| 6. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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Characterization of mixed disomic and polysomic inheritance in the octoploid strawberry (Fragaria x ananassa) using AFLP mapping
- 2003
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Ingår i: Theoretical and Applied Genetics. - : SPRINGER-VERLAG. - 0040-5752 .- 1432-2242. ; 107:4, s. 619-628
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Tidskriftsartikel (refereegranskat)abstract
- A two-way pseudo-testcross strategy, combined with Single Dose Restriction Fragment (SDRF) marker analysis, was used for genetic mapping in the octoploid cultivated strawberry Fragaria x ananassa (2n=8x=56). Based on a 113 full-sib progeny from a cross between the variety Capitola and the clone CF1116, we generated two parental maps using Amplified Fragment Length Polymorphism (AFLP) markers. Ninety two percent of the markers (727 out of 789) showed ratios corresponding to simplex markers (the majority being SDRF markers), and 8% (62 out of 789) fitted a multiplex ratio. Linkage maps were first established using SDRF markers in coupling phase. The female map comprised 235 markers distributed among 43 co-segregation groups, giving a map size of 1,604 cM. On the male map, 280 markers were assigned to 43 co-segregation groups, yielding a map size of 1,496 cM. Once the co-segregation groups were established, their association was tested using repulsion-phase markers. In total, taking into account associations representing the same linkage groups, 30 linkage groups were detected on the female side and 28 on the male side. On the female map, 68.3% of the pairwise marker linkages were in coupling versus 31.7% in repulsion phase, and the corresponding figures on the male map were 72.2% and 27.8%, respectively. In addition, both groups linked only in the coupling phase and groups linked in the repulsion phase were characterized. The observations suggest that the meiotic behavior of the F. x ananassa genome is neither fully disomic nor fully polysomic, but rather mixed. The genome may not be as completely diploidized as previously assumed.
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| 7. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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Development of a multiplex PCR microsatellite assay in brown trout Salmo trutta, and its potential application for the genus
- 2006
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Ingår i: Aquaculture. - : ELSEVIER. - 0044-8486 .- 1873-5622. ; 258:1-4, s. 641-645
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Tidskriftsartikel (refereegranskat)abstract
- The use of multiplex PCR assays to screen microsatellite variation is a rapid, economically efficient and robust approach to acquire population genetic data. To date, however, few such multiplex assays have been reported in large-scale studies of a fishery. We developed a two reaction, 12 locus microsatellite assay for brown trout, one of the most important aquaculture species in Europe. We describe the steps taken to choose loci that provide unambiguous data in an applied fisheries context. Several parameters were tested for assay optimisation, among which the relative concentration of each primer pair and the PCR elongation time and temperature were the most important. The assay was optimised for the two lineages of brown trout (Atlantic and Danubian) found in Austria. Evaluation on additional lineages of Salmo trutta as well as other Salmo species showed that with few adjustments the assay could be reliably used in all lineages of brown trout as well as S. marmoratus, S. ohridanus, and S. obtusirostris, but probably not for S. salar without major change. (c) 2006 Elsevier B.V. All rights reserved.
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| 8. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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Development of a SCAR marker linked to dominant gene conferring resistance to Colletotrichum acutatum in strawberry (Fragaria x ananassa)
- 2003
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Ingår i: BERRY CROP BREEDING, PRODUCTION AND UTILIZATION FOR A NEW CENTURY. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 85-91
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Konferensbidrag (refereegranskat)abstract
- Colletotrichum acutatum is responsible for strawberry anthracnose, one of the main disease of cultivated strawberry in the south-western area of France (60% of the French production). Anthracnose is a polycyclic disease for which no efficient disease control method has been developed. Therefore, the development of cultivars with sustainable resistance, i.e. efficient against the two pathogenicity groups, may be promising to lower both the level of damage and the use of chemicals. The objective of this study is to identify molecular markers linked to the major dominant gene conferring resistance to pathogenicity group 2 by using an approach of Bulk Segregant Analysis (BSA) and Amplified Fragment Length Polymorphisin (AFLP) markers. We identified four molecular markers, named mf1 to mf4, linked to the resistance gene to the pathogenicity group 2 of C. acutatum. Within a segregating population (back cross type) for the dominant gene, no recombination between the resistance allele and the marker mf3 was detected in 78 BC1 individuals studied. One Sequence Characterized Amplified Region (SCAR) obtained from mf4 (SCAR-mf4) provided a fast and easy test for selection of anthracnose resistant plants at early stage, with a misidentification rate of 1.3%. The evaluation of this SCAR marker in few strawberry varieties showed that only some resistant varieties possessed this marker.
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| 10. |
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