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- 2019
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Tidskriftsartikel (refereegranskat)
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- Menkveld, Albert J., et al.
(författare)
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Nonstandard Errors
- 2024
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Ingår i: JOURNAL OF FINANCE. - : Wiley-Blackwell. - 0022-1082 .- 1540-6261. ; 79:3, s. 2339-2390
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Tidskriftsartikel (refereegranskat)abstract
- In statistics, samples are drawn from a population in a data-generating process (DGP). Standard errors measure the uncertainty in estimates of population parameters. In science, evidence is generated to test hypotheses in an evidence-generating process (EGP). We claim that EGP variation across researchers adds uncertainty-nonstandard errors (NSEs). We study NSEs by letting 164 teams test the same hypotheses on the same data. NSEs turn out to be sizable, but smaller for more reproducible or higher rated research. Adding peer-review stages reduces NSEs. We further find that this type of uncertainty is underestimated by participants.
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| 4. |
- Hao, Meng-Shu, et al.
(författare)
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Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans
- 2024
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Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 15:1
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Tidskriftsartikel (refereegranskat)abstract
- Carbohydrate-binding modules (CBMs) are non-catalytic proteins found appended to carbohydrate-active enzymes. Soil and marine bacteria secrete such enzymes to scavenge nutrition, and they often use CBMs to improve reaction rates and retention of released sugars. Here we present a structural and functional analysis of the recently established CBM family 92. All proteins analysed bind preferentially to β−1,6-glucans. This contrasts with the diversity of predicted substrates among the enzymes attached to CBM92 domains. We present crystal structures for two proteins, and confirm by mutagenesis that tryptophan residues permit ligand binding at three distinct functional binding sites on each protein. Multivalent CBM families are uncommon, so the establishment and structural characterisation of CBM92 enriches the classification database and will facilitate functional prediction in future projects. We propose that CBM92 proteins may cross-link polysaccharides in nature, and might have use in novel strategies for enzyme immobilisation.
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| 5. |
- Jiao, Xingxing, et al.
(författare)
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Crumpled Nitrogen-Doped Graphene-Wrapped Phosphorus Composite as a Promising Anode for Lithium-Ion Batteries
- 2019
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Ingår i: ACS Applied Materials & Interfaces. - : American Chemical Society (ACS). - 1944-8252 .- 1944-8244. ; 11:34, s. 30858-30864
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Tidskriftsartikel (refereegranskat)abstract
- Red phosphorus (P) has recently gained wide attention because of the high theoretical capacity of 2596 mA h/g, which has been regarded as promising anode material for lithium-ion batteries (LIBs). However, the actual application of red P in LIBs is hampered by the huge expansion of volume and low electronic conductivity. Herein, we design a kind of red phosphorus/crumpled nitrogen-doped graphene (P/CNG) nanocomposites with high capacity density and great rate performance as anode material for LIBs. This anode material was rationally fabricated through the scalable ball-milling method. The nanocomposite structure of P/CNG improves the electron conductivity and alleviates volume change of raw red P because of the three-dimension (3D) framework, massive defects and active sites of CNG sheets. As expected, the P/CNG composite shows excellent electrochemical performances, including high capacity (2522.6 mA h/g at 130 mA/g), remarkable rate capability (1340.5 mA h/g at 3900 mA/g), and great cyclability (1470.1 mA h/g at 1300 mA/g for 300 cycles). This work may provide a broad prospect for a great rate performance of P-based anode material for LIBs.
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| 6. |
- Li, He, D.Sc, 1985-, et al.
(författare)
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Family 92 carbohydrate-binding modules specific for β-1,6-glucans increase the thermostability of a bacterial chitinase
- 2023
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Ingår i: Biochimie. - : Elsevier BV. - 0300-9084 .- 1638-6183. ; 212, s. 153-160
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Tidskriftsartikel (refereegranskat)abstract
- In biomass-processing industries there is a need for enzymes that can withstand high temperatures. Extensive research efforts have been dedicated to finding new thermostable enzymes as well as developing new means of stabilising existing enzymes. The attachment of a stable non-catalytic domain to an enzyme can, in some instances, protect a biocatalyst from thermal denaturation. Carbohydrate-binding modules (CBMs) are non-catalytic domains typically found appended to biomass-degrading or modifying enzymes, such as glycoside hydrolases (GHs). Most often, CBMs interact with the same polysaccharide as their enzyme partners, leading to an enhanced reaction rate via the promotion of enzyme-substrate interactions. Contradictory to this general concept, we show an example of a chitin-degrading enzyme from GH family 18 that is appended to two CBM domains from family 92, both of which bind preferentially to the non-substrate polysaccharide β-1,6-glucan. During chitin hydrolysis, the CBMs do not contribute to enzyme-substrate interactions but instead confer a 10–15 °C increase in enzyme thermal stability. We propose that CBM92 domains may have a natural enzyme stabilisation role in some cases, which may be relevant to enzyme design for high-temperature applications in biorefinery.
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| 7. |
- Li, He, D.Sc, 1985-, et al.
(författare)
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Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3, 5-Dinitrosalicylic Acid Assay
- 2023. - 2
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Ingår i: Carbohydrate-Protein Interactions. - New York : Springer Nature. ; 2657, s. 15-25
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Bokkapitel (refereegranskat)abstract
- Use of the 3,5-dinitrosalicylic acid reagent allows the simple, rapid quantification of reducing sugars. The method can be used for analysis of biological samples or in characterization of enzyme reactions, as new reducing ends are generated when a polysaccharide substrate undergoes hydrolytic cleavage. Presented here is an application of the method in measuring the kinetics of a glycoside hydrolase reaction, including the optimization of the DNSA reagent, and the production of a standard curve of absorbance versus sugar concentration.
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| 8. |
- Lu, Zijia, et al.
(författare)
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A polysaccharide utilization locus from Chitinophaga pinensis simultaneously targets chitin and β-glucans found in fungal cell walls
- 2023
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Ingår i: mSphere. - : American Society for Microbiology. - 2379-5042.
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Tidskriftsartikel (refereegranskat)abstract
- In nature, complex carbohydrates are rarely found as pure isolated polysaccharides. Instead, bacteria in competitive environments are presented with glycans embedded in heterogeneous matrices such as plant or microbial cell walls. Members of the Bacteroidota phylum thrive in such ecosystems because they are efficient at extracting nutrients from complex substrates, secreting consortia of synergistic enzymes to release metabolizable sugars. Carbohydrate-binding modules (CBMs) are used to target enzymes to substrates, enhancing reaction rate and product release. Additionally, genome organizational tools like polysaccharide utilization loci (PULs) ensure that the appropriate set of enzymes is produced when needed. In this study, we show that the soil bacterium Chitinophaga pinensis uses a PUL and several CBMs to coordinate the activities of enzymes targeting two distinct polysaccharides found in fungal cell walls. We describe the enzymatic activities and carbohydrate-binding behaviors of components of the fungal cell wall utilization locus (FCWUL), which uses multiple chitinases and one β-1,3-glucanase to hydrolyze two different substrates. Unusually, one of the chitinases is appended to a β-glucan-binding CBM, implying targeting to a bulk cell wall substrate rather than to the specific polysaccharide being hydrolyzed. Based on our characterization of the PUL’s outer membrane sensor protein, we suggest that the FCWUL is activated by β-1,3-glucans, even though most of its enzymes are chitin-degrading. Our data showcase the complexity of polysaccharide deconstruction in nature and highlight an elegant solution for how multiple different glycans can be accessed using one enzymatic cascade.
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| 9. |
- Guan, Ke, et al.
(författare)
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Towards realistic high-speed train channels at 5G millimeter-wave band - Part I: Paradigm, significance analysis, and scenario reconstruction
- 2018
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Ingår i: IEEE Transactions on Vehicular Technology. - 0018-9545 .- 1939-9359. ; 67:10, s. 9112-9128
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Tidskriftsartikel (refereegranskat)abstract
- The upcoming fifth-generation (5G) mobile communication system is expected to support high mobility up to 500 km/h, which is envisioned in particular for high-speed trains. Millimeter wave (mmWave) spectrum is considered as a key enabler for offering the 'best experience' to highly mobile users. Despite that channel characterization is necessary for the mmWave system design and validation, it is still not feasible to directly do extensive mmWave mobile channel measurements on moving high-speed trains (HST) at a speed up to 500 km/h in the present. Thus, rather than conducting mmWave HST channel sounding directly with high mobility, this study proposes a viable paradigm for realizing the realistic HST channels at the 5G mmWave band. We first propose the whole paradigm. Then, we define the scenario of interest and select the main objects and materials. Afterwards, the electromagnetic and scattering parameters of the materials are measured and estimated between 26.5 GHz and 40 GHz. With this information, the most influential materials are determined through significance analysis. Correspondingly, we reconstruct the three-dimensional mmWave outdoor HST and tunnel scenario models. Through extensive ray-tracing simulations, we determine the main propagation mechanisms in these two scenarios, the channel models based on that are validated by measurements. This verifies the whole paradigm proposed in this paper.
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| 10. |
- Guan, Ke, et al.
(författare)
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Towards realistic high-speed train channels at 5G millimeter-wave band - Part II: Case study for paradigm implementation
- 2018
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Ingår i: IEEE Transactions on Vehicular Technology. - 0018-9545 .- 1939-9359. ; 67:10, s. 9129-9144
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Tidskriftsartikel (refereegranskat)abstract
- © 1967-2012 IEEE. In this paper, we present two case studies for generating realistic high-speed train (HST) channels at fifth-generation (5G) millimeter-wave (mmWave) band. The first one is the tunnel environment at relatively low 5G mmWave band, 30 GHz band, whereas the second one is the outdoor HST environment at relatively high 5G mmWave band, 90 GHz band. Both case studies include the following steps: ray-tracing simulations, stochastic channel modeling and realization, verification with ray-tracing simulations, and validation with a reduced set of measurements. A profound and insightful conclusion is reached that by employing the proposed paradigm, realistic channels can be realized for the design and evaluation of 5G mmWave communication systems in high-speed railways, even without the support of sufficient channel sounding data.
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