| 1. |
- Li, He, et al.
(författare)
-
Electrochemical immunosensor with N-doped graphene-modified electrode for label-free detection of the breast cancer biomarker CA 15-3
- 2013
-
Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 43, s. 25-29
-
Tidskriftsartikel (refereegranskat)abstract
- Highly sensitive and label-free detection of the biomarker carbohydrate antigen 15-3 (CA 15-3) remains a challenge in the diagnosis of breast cancer. Here, a novel electrochemical immunosensor capable of sensitive and label-free detection of CA 15-3 is reported. This unique immunosensor, equipped with a highly conductive graphene (i.e., N-doped graphene sheets)-modified electrode, exhibited significantly increased electron transfer and high sensitivity toward CA 15-3. This novel immunosensor, with a low detection limit of 0.012U/mL, worked well over a broad linear range of 0.1-20U/mL. Unlike conventional immunosensors, which usually involve complicated label processing and time-consuming separations, the use of highly conductive graphene avoids the need for labels and is simple in nature. The strategy developed for this immunosensor provides a promising approach for clinical research and diagnostic applications.
|
|
| 2. |
- Li, Songjun, et al.
(författare)
-
A Catalytic and Positively Thermosensitive Molecularly Imprinted Polymer
- 2011
-
Ingår i: Advanced Functional Materials. - : John Wiley and Sons, Ltd. - 1616-301X .- 1616-3028. ; 21:6, s. 1194-1200
-
Tidskriftsartikel (refereegranskat)abstract
- A catalytic and positively thermosensitive molecularly imprinted polymer is reported. This unique imprinted polymer was composed of 4-nitrophenyl phosphate-imprinted networks that exhibited a thermosensitive interpolymer interaction between poly(2-trifluoromethylacrylic acid) (PTFMA) and poly(1-vinylimidazole) (PVI), which contains catalytically active sites. At a relatively low temperature (such as 20 degrees C), this imprinted polymer did not demonstrate significant catalytic activity for the hydrolysis of 4-nitrophenyl acetate due to the interpolymer complexation between PVI and PTFMA, which blocked access to the active sites of PVI and caused shrinking of the polymer. Conversely, at higher temperatures (such as 40 degrees C), this polymer showed significant catalytic activity resulting from the dissociation of the interpolymer complexes between PVI and PTFMA, which facilitated access to the active sites of PVI and inflated the polymer. Unlike previously reported poly(N-isopropylacrylamide)-based molecularly imprinted polymers, which demonstrated decreased molecular recognition and catalytic activity with increased temperatures, i.e., negatively thermosensitive molecular recognition and catalysis abilities, this imprinted polymer exploits the unique interpolymer interaction between PVI and PTFMA, enabling the reversed thermal responsiveness.
|
|
| 3. |
- Li, Songjun, et al.
(författare)
-
A Zipper-Like On/Off-Switchable Molecularly Imprinted Polymer
- 2011
-
Ingår i: Advanced Functional Materials. - : Wiley-VCH Verlag Berlin. - 1616-301X .- 1616-3028. ; 21:17, s. 3344-3349
-
Tidskriftsartikel (refereegranskat)abstract
- A zipper-like on/off-switchable molecularly imprinted polymer is reported. This unique imprinted polymer was composed of template-imprinted polymeric networks that incorporate zipper-like interactions between poly(acrylamide) (PAAm) and poly(2-acrylamide-2-methyl propanesulfonic acid) (PAMPS). This polymer showed marginal recognition ability towards the imprint species under low temperature conditions, due to the interpolymer interaction between PAAm and PAMPS, which inhibited access to the imprinted networks. In contrast, at relatively high temperatures (such as 40 degrees C), the polymer demonstrated significant molecular recognition ability towards the imprint species resulting from the dissociation of the interpolymer complexes of PAAm and PAMPS, which enabled access to the imprint networks. Unlike previously reported PNIPAm-based imprinted polymers, which demonstrate alterable molecular recognition simply because of the thermosensitive hydrophilicity/hydrophobicity of PNIPAm, this polymer employed a zipper-like supramolecular architecture between PAAm and PAMPS, thereby enabling switchable molecular recognition.
|
|
| 4. |
- Li, Songjun, et al.
(författare)
-
Baltic Sea coastal sediment-bound eukaryotes have increased year-round activities under predicted climate change related warming
- 2024
-
Ingår i: Frontiers in Microbiology. - : Frontiers Media S.A.. - 1664-302X. ; 15
-
Tidskriftsartikel (refereegranskat)abstract
- Climate change related warming is a serious environmental problem attributed to anthropogenic activities, causing ocean water temperatures to rise in the coastal marine ecosystem since the last century. This particularly affects benthic microbial communities, which are crucial for biogeochemical cycles. While bacterial communities have received considerable scientific attention, the benthic eukaryotic community response to climate change remains relatively overlooked. In this study, sediments were sampled from a heated (average 5°C increase over the whole year for over 50 years) and a control (contemporary conditions) Baltic Sea bay during four different seasons across a year. RNA transcript counts were then used to investigate eukaryotic community changes under long-term warming. The composition of active species in the heated and control bay sediment eukaryotic communities differed, which was mainly attributed to salinity and temperature. The family level RNA transcript alpha diversity in the heated bay was higher during May but lower in November, compared with the control bay, suggesting altered seasonal activity patterns and dynamics. In addition, structures of the active eukaryotic communities varied between the two bays during the same season. Hence, this study revealed that long-term warming can change seasonality in eukaryotic diversity patterns. Relative abundances and transcript expression comparisons between bays suggested that some taxa that now have lower mRNA transcripts numbers could be favored by future warming. Furthermore, long-term warming can lead to a more active metabolism in these communities throughout the year, such as higher transcript numbers associated with diatom energy production and protein synthesis in the heated bay during winter. In all, these data can help predict how future global warming will affect the ecology and metabolism of eukaryotic community in coastal sediments.
|
|
| 5. |
|
|
| 6. |
- Li, Songjun, et al.
(författare)
-
Foreward
- 2015
-
Ingår i: Molecularly Imprinted Catalysts<em> </em>. - : Elsevier. - 9780128013014 ; , s. xvii-xix
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)
|
|
| 7. |
- Li, Songjun, et al.
(författare)
-
Molecularly Imprinted Polymers for Enzyme-Like Catalysis : principle, design and applications
- 2015
-
Ingår i: Molecularly imprinted polymers for enzyme-like catalysis<em> </em>. - : Elsevier. - 9780128013014 - 9780128014448 ; , s. 1-17
-
Bokkapitel (refereegranskat)abstract
- Selective catalysis remains a significant challenge owing to the lack of a generic protocol suitable for the preparation of selective catalytic materials. A promising approach is to translate the principle of enzyme catalysis for the design of new catalytic materials. Known as a “key-to-lock” technology, molecular imprinting provides a promising perspective by helping create in a straightforward manner binding sites that possess enzyme-like catalytic ability with but higher stability. In this chapter, we focus on discussing some key issues involved in active molecularly imprinted polymers from catalytic applications. The similarity and difference between preparing conventional molecularly imprinted polymers and catalytic imprinted polymers are highlighted. Other aspects relating to the principle, design, and future outlook of catalytic molecularly imprinted polymers are also discussed.
|
|
| 8. |
- Li, Songjun, et al.
(författare)
-
‘On/off’-switchable catalysis by a smart enzyme-like imprinted polymer
- 2011
-
Ingår i: Journal of Catalysis. - : Elsevier. - 0021-9517 .- 1090-2694. ; 278:2, s. 173-180
-
Tidskriftsartikel (refereegranskat)abstract
- ‘On/off’-switchable catalysis by a smart enzyme-like imprinted polymer is reported. This unique imprinted polymer was composed of poly(N-isopropylacrylamide)-containing p-nitrophenyl phosphate-imprinted networks that exhibited temperature-dependent hydrophilicity/hydrophobicity. At a relatively low temperature (such as 20 °C), this polymer was capable of vigorous catalysis for the hydrolysis of p-nitrophenyl acetate due to its hydrophilic networks, which enabled access to the imprinted framework. On the contrary, at higher temperatures (such as 40 °C), this polymer demonstrated poor catalysis resulting from its dramatically increased hydrophobicity, which inhibited access to the imprinted sites. Unlike previously reported imprinted polymers which lack adjustable networks, this novel imprinted polymer employed thermosensitive poly(N-isopropylacrylamide) networks, thus enabling the switchable catalysis.
|
|
