| 1. |
|
|
| 2. |
- Leebens-Mack, James H., et al.
(författare)
-
One thousand plant transcriptomes and the phylogenomics of green plants
- 2019
-
Ingår i: Nature. - : Nature Publishing Group. - 0028-0836 .- 1476-4687. ; 574:7780, s. 679-
-
Tidskriftsartikel (refereegranskat)abstract
- Green plants (Viridiplantae) include around 450,000-500,000 species(1,2) of great diversity and have important roles in terrestrial and aquatic ecosystems. Here, as part of the One Thousand Plant Transcriptomes Initiative, we sequenced the vegetative transcriptomes of 1,124 species that span the diversity of plants in a broad sense (Archaeplastida), including green plants (Viridiplantae), glaucophytes (Glaucophyta) and red algae (Rhodophyta). Our analysis provides a robust phylogenomic framework for examining the evolution of green plants. Most inferred species relationships are well supported across multiple species tree and supermatrix analyses, but discordance among plastid and nuclear gene trees at a few important nodes highlights the complexity of plant genome evolution, including polyploidy, periods of rapid speciation, and extinction. Incomplete sorting of ancestral variation, polyploidization and massive expansions of gene families punctuate the evolutionary history of green plants. Notably, we find that large expansions of gene families preceded the origins of green plants, land plants and vascular plants, whereas whole-genome duplications are inferred to have occurred repeatedly throughout the evolution of flowering plants and ferns. The increasing availability of high-quality plant genome sequences and advances in functional genomics are enabling research on genome evolution across the green tree of life.
|
|
| 3. |
- de las Fuentes, Lisa, et al.
(författare)
-
Gene-educational attainment interactions in a multi-ancestry genome-wide meta-analysis identify novel blood pressure loci
- 2021
-
Ingår i: Molecular Psychiatry. - : Springer Nature. - 1359-4184 .- 1476-5578. ; 26:6, s. 2111-2125
-
Tidskriftsartikel (refereegranskat)abstract
- Educational attainment is widely used as a surrogate for socioeconomic status (SES). Low SES is a risk factor for hypertension and high blood pressure (BP). To identify novel BP loci, we performed multi-ancestry meta-analyses accounting for gene-educational attainment interactions using two variables, “Some College” (yes/no) and “Graduated College” (yes/no). Interactions were evaluated using both a 1 degree of freedom (DF) interaction term and a 2DF joint test of genetic and interaction effects. Analyses were performed for systolic BP, diastolic BP, mean arterial pressure, and pulse pressure. We pursued genome-wide interrogation in Stage 1 studies (N = 117 438) and follow-up on promising variants in Stage 2 studies (N = 293 787) in five ancestry groups. Through combined meta-analyses of Stages 1 and 2, we identified 84 known and 18 novel BP loci at genome-wide significance level (P < 5 × 10-8). Two novel loci were identified based on the 1DF test of interaction with educational attainment, while the remaining 16 loci were identified through the 2DF joint test of genetic and interaction effects. Ten novel loci were identified in individuals of African ancestry. Several novel loci show strong biological plausibility since they involve physiologic systems implicated in BP regulation. They include genes involved in the central nervous system-adrenal signaling axis (ZDHHC17, CADPS, PIK3C2G), vascular structure and function (GNB3, CDON), and renal function (HAS2 and HAS2-AS1, SLIT3). Collectively, these findings suggest a role of educational attainment or SES in further dissection of the genetic architecture of BP.
|
|
| 4. |
- Kanoni, Stavroula, et al.
(författare)
-
Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis.
- 2022
-
Ingår i: Genome biology. - : Springer Science and Business Media LLC. - 1474-760X .- 1465-6906 .- 1474-7596. ; 23:1
-
Tidskriftsartikel (refereegranskat)abstract
- Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery.To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N=1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3-5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism.Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk.
|
|
| 5. |
- Huang, Hongyun, et al.
(författare)
-
Clinical Cell Therapy Guidelines for Neurorestoration (IANR/CANR 2017)
- 2018
-
Ingår i: Cell Transplantation. - : SAGE Publications. - 0963-6897 .- 1555-3892. ; 27:2, s. 310-324
-
Forskningsöversikt (refereegranskat)abstract
- Cell therapy has been shown to be a key clinical therapeutic option for central nervous system diseases or damage. Standardization of clinical cell therapy procedures is an important task for professional associations devoted to cell therapy. The Chinese Branch of the International Association of Neurorestoratology (IANR) completed the first set of guidelines governing the clinical application of neurorestoration in 2011. The IANR and the Chinese Association of Neurorestoratology (CANR) collaborated to propose the current version "Clinical Cell Therapy Guidelines for Neurorestoration (IANR/CANR 2017)". The IANR council board members and CANR committee members approved this proposal on September 1, 2016, and recommend it to clinical practitioners of cellular therapy. These guidelines include items of cell type nomenclature, cell quality control, minimal suggested cell doses, patient-informed consent, indications for undergoing cell therapy, contraindications for undergoing cell therapy, documentation of procedure and therapy, safety evaluation, efficacy evaluation, policy of repeated treatments, do not charge patients for unproven therapies, basic principles of cell therapy, and publishing responsibility.
|
|
| 6. |
- Li, Peng, et al.
(författare)
-
Transcriptional reactivation of OTX2, RX1 and SIX3 during reprogramming contributes to the generation of RPE cells from human iPSCs
- 2016
-
Ingår i: International Journal of Biological Sciences. - : Ivyspring International Publisher. - 1449-2288. ; 12:5, s. 505-517
-
Tidskriftsartikel (refereegranskat)abstract
- Directed differentiation of human induced pluripotent stem cells (iPSCs) into retinal pigmented epithelium (RPE) holds great promise in cell replacement therapy for patients suffering from degenerative eye diseases, including age-related macular degeneration (AMD). In this study, we generated iPSCs from human dermal fibroblasts (HDFs) by electroporation with episomal plasmid vectors encoding OCT4, SOX2, KLF4, L-MYC together with p53 suppression. Intriguingly, cell reprogramming resulted in a metastable transcriptional activation and selective demethylation of neural and retinal specification-associated genes, such as OTX2, RX1 and SIX3. In contrast, RPE progenitor genes were transcriptionally silent in HDFs and descendant iPSCs. Overexpression of OCT4 and SOX2 directly stimulated the expression of OTX2, RX1 and SIX3 in HDFs and iPSCs. Luciferase and chromatin immunoprecipitation (ChIP) assays further identified an OCT4- and two SOX2-binding sites located in the proximal promoter of OTX2. Histone acetylation and methylation on the local promoter also participated in the reactivation of OTX2. The transcriptional conversion of RX1 and SIX3 genes partially attributed to DNA demethylation. Subsequently, iPSCs were induced into the RPE cells displaying the characteristics of polygonal shapes and pigments, and expressing typical RPE cell markers. Taken together, our results establish readily efficient and safe protocols to produce iPSCs and iPSC-derived RPE cells, and underline that the reactivation of anterior neural transcription factor OTX2, eye field transcription factor RX1 and SIX3 in iPSCs is a feature of pluripotency acquisition and predetermines the potential of RPE differentiation.
|
|
| 7. |
- Li, Xiaofeng, et al.
(författare)
-
双色光束增强飞秒激光氮气标记示踪测速技术的研究
- 2022
-
Ingår i: Kung Cheng Je Wu Li Hsueh Pao/Journal of Engineering Thermophysics. - 0253-231X. ; 43:10, s. 2826-2830
-
Tidskriftsartikel (refereegranskat)abstract
- In this work, the fluorescence of FLEET (femtosecond laser electron excited tagging) was enhanced by two color femtosecond laser. the intensity of FLEET was increased about 20% with two laser pulses that were 400 μJ/pulse at 266 nm and 3 mJ/pulse at 800 nm, and the results indicated that there was nearly no influence on lifetime and wavelength of fluorescence from FLEET. This result provides many information for the following studies.
|
|
| 8. |
- Gao, Qiang, et al.
(författare)
-
Femtosecond-laser electronic-excitation tagging velocimetry using a 267 nm laser
- 2019
-
Ingår i: Sensors and Actuators, A: Physical. - : Elsevier BV. - 0924-4247. ; 287, s. 138-142
-
Tidskriftsartikel (refereegranskat)abstract
- Femtosecond (fs)-laser electronic-excitation tagging velocimetry (FLEET) in a nitrogen flow field using a 267 nm laser was performed under the condition of fs-laser filamentation. The filamentous properties and their effects on velocity measurements were investigated and were compared with those of an 800 nm fs-laser. The results show that the required energy of the 267 nm laser pulse is as low as hundreds of μJ, and this is beneficial for reducing the potential perturbations to the flow flied. The filaments induced by the 267 nm laser are longer and thinner than are those induced by the 800 nm laser, which enlarges the velocity measurements region, and a precision of 1.3% was achieved.
|
|
| 9. |
- Li, Bo, et al.
(författare)
-
Comprehensive CO detection in flames using femtosecond two-photon laser-induced fluorescence
- 2017
-
Ingår i: Optics Express. - 1094-4087. ; 25:21, s. 25809-25818
-
Tidskriftsartikel (refereegranskat)abstract
- We demonstrate a femtosecond two-photon laser-induced fluorescence (fs-TPLIF) technique for sensitive CO detection, using a 230 nm pulse of 9 µJ and 45 fs. The advantages of fs-TPLIF in excitation of molecular species were analyzed. Spectra of CO fs-TPLIF were recorded in stable laminar flames spatially resolved across the flame front. A hot band (1, n) together with the conventional band (0, n) of the B→A transitions were observed in the burned zone and attributed to the broadband nature of the fs excitation. The CO fs-TPLIF signal recorded across the focal point of the excitation beam shows a relatively flat intensity distribution despite of the steep laser intensity variation, which is beneficial for CO imaging in contrast to nanosecond and picosecond TPLIF. This phenomenon can be explained by photoionization, which over the short pulse duration dominates the population depletion of the excited B state due to the high peak power, but only contributes in total a negligible X state depletion due to the low pulse energy. Single-shot CO fs-TPLIF images in methane/air flames were recorded by imaging the broadband fluorescence. The results indicate that fs-TPLIF is a promising tool for CO imaging in flames.
|
|
| 10. |
- Li, Bo, et al.
(författare)
-
Femtosecond laser-induced cyano chemiluminescence in methane-seeded nitrogen gas flows for near-wall velocimetry
- 2018
-
Ingår i: Journal of Physics D: Applied Physics. - : IOP Publishing. - 0022-3727 .- 1361-6463. ; 51:29
-
Tidskriftsartikel (refereegranskat)abstract
- We demonstrate a velocimetric technique based on femtosecond laser-induced cyano (CN) chemiluminescence (FLICC). High intensity emission originated from CN(B-X) fluorescence was observed in filaments generated by focusing a femtosecond laser in methane-seeded nitrogen gas flows. The emission is strong and can last for hundreds of microseconds with a proper methane concentration. FLICC was adopted for velocity measurements, and promising results were obtained for near-wall measurements.
|
|
