| 1. |
- Ståhl, Patrik, Dr., et al.
(författare)
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Visualization and analysis of gene expression in tissue sections by spatial transcriptomics
- 2016
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Ingår i: Science. - : AMER ASSOC ADVANCEMENT SCIENCE. - 0036-8075 .- 1095-9203. ; 353:6294, s. 78-82
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Tidskriftsartikel (refereegranskat)abstract
- Analysis of the pattern of proteins or messenger RNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics. This typically involves the visualization of a few proteins or expressed genes at a time. We have devised a strategy, which we call "spatial transcriptomics," that allows visualization and quantitative analysis of the transcriptome with spatial resolution in individual tissue sections. By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, we demonstrate high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer. Spatial transcriptomics provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and enables novel types of bioinformatics analyses, valuable in research and diagnostics.
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| 2. |
- Cheng, Peng, et al.
(författare)
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A Local Positioning System for Loader Cranes Based on Wireless Sensors-A Feasibility Study
- 2011
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Ingår i: IEEE Transactions on Instrumentation and Measurement. - 0018-9456 .- 1557-9662. ; 60:8, s. 2881-2893
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents a feasibility study that deals with a local positioning system for a loader crane based on battery-powered wireless sensors and consists of two joint angular sensors and one telescopic boom length ranger. The practical challenges associated with using conventional sensors are described in order to provide the motivation behind the choice to use the sensing methods proposed in this paper. A novel method is tested using microelectromechanical system inertial sensors mounted around the crane joints to indirectly measure the joint angles, as well as an ultrasound time-of-flight ranging method to measure the telescopic boom length. The local positioning system's wireless sensor prototype designs are described in detail. Data from the angular sensor experiments conducted on a loader crane and the ultrasound ranging experiments conducted both in the laboratory and on the loader crane are presented and analyzed. The preliminary results from these experiments show that the performance of the new sensors is promising. The conclusion is drawn from the experimental results, and future work for this local positioning system is also described.
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| 3. |
- Cheng, Peng, et al.
(författare)
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Joint Angular Sensor Based on Distributed Biaxial MEMS Accelerometers
- 2007
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Ingår i: IECON 2007: 33RD ANNUAL CONFERENCE OF THE IEEE INDUSTRIAL ELECTRONICS SOCIETY, VOLS 1-3, CONFERENCE PROCEEDINGS. - New York : IEEE conference proceedings. - 1424407834 ; , s. 2242-2247
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Konferensbidrag (refereegranskat)abstract
- This paper presents the analysis for joint angle measurement on rigid body that is based on distributed biaxial MEMS accelerometers. It focuses on two methods, one called CMR and another DCMR, and utilizes the property of rigid body kinematics to explain their advantages and weaknesses. Unlike CMR method, DCMR method has no requirement on placing the sensors close to the joint center. This provides greater flexibility for the sensor installation. On the basis of the error model of CMR method, we give an analysis outlining the advantage of theoretically error-free DCMR method. The sensor calibration and alignment is described and both methods are characterized on a rigid body robot arm model. The experiment shows the angular error up to 0.4rad from CMR method whereas just 0.03rad from DCMR method. The noise level from both methods is also compared and analyzed. The result reveals a larger but tunable noise for DCMR method.
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| 4. |
- Hallén, Anders., et al.
(författare)
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Ion implantation of silicon carbide
- 2002
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Ingår i: Nuclear Instruments and Methods in Physics Research Section B. - 0168-583X .- 1872-9584. ; 186, s. 186-194
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Tidskriftsartikel (refereegranskat)abstract
- Ion implantation is an important technique for a successful implementation of commercial SiC devices. Much effort has also been devoted to optimising implantation and annealing parameters to improve the electrical device characteristics. However, there is a severe lack of understanding of the fundamental implantation process and the generation and annealing kinetics of point defects and defect complexes. Only very few of the most elementary intrinsic point defects have been unambiguously identified so far. To reach a deeper understanding of the basic mechanisms SiC samples have been implanted with a broad range of ions, energies, doses, etc., and the resulting defects and damage produced in the lattice have been studied with a multitude of characterisation techniques. In this contribution we will review some of the results generated recently and also try to indicate where more research is needed. In particular, deep level transient spectroscopy (DLTS) has been used to investigate point defects at very low doses and transmission electron microscopy (TEM) and Rutherford backscattering spectrometry (RBS) are used for studying the damage build-up at high doses.
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| 5. |
- Linnarsson, Fredrik, et al.
(författare)
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A Power Aware MAC protocol for Wireless Closed Loop Control System in Loader Cranes
- 2008
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Ingår i: 2008 IEEE INTERNATIONAL SYMPOSIUM ON INDUSTRIAL ELECTRONICS, VOLS 1-5. - New York : IEEE conference proceedings. - 9781424416653 ; , s. 1806-1811
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Konferensbidrag (refereegranskat)abstract
- This paper will discuss a wireless control system for a loader crane, where the focus lies on the wireless communication protocol supporting the implementation of the real-time system. To aid the discussion, a system model is included, which together with the control strategy will allow low power consumption for the energy constraint sensor devices. A simple and power aware medium access control protocol is proposed. The protocol together with the specific loader crane requirements make it possible to save valuable sensor resources. Simulations on real world loader crane data show a simplified view of the power requirements for the control model, and the importance of utilizing correct system parameters to achieve low power consumption.
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| 6. |
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| 7. |
- Linnarsson, Fredrik, et al.
(författare)
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Analysis of the IEEE 802.15.4 Standard for a Wireless Closed Loop Control System for Heavy Duty Cranes
- 2007
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Ingår i: 2007 INTERNATIONAL SYMPOSIUM ON INDUSTRIAL EMBEDDED SYSTEMS - SIES'2007. - : IEEE conference proceedings. - 9781424408399 ; , s. 164-169
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Konferensbidrag (refereegranskat)abstract
- In this paper the use of the IIEEE 802.15.4 standard in a wireless control system is analyzed and discussed. The standard is described and applied on a heavy duty crane application and the discussion focuses on the possibilities and limitations of the standard in the application. A formula is presented which can be used to calculate maximum sample rate according to the number of sensor nodes and the payload the nodes may carry. The discussion reveals that the standard may be utilized in the crane application but that it is not a good solution. Several limitations within the standard, such as network size and system sample rate, will put a stop to future extensions of the wireless control system. As a result, the 802.15.4 standard protocol shall not be employed in the heavy duty crane application.
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| 8. |
- Linnarsson, Fredrik, et al.
(författare)
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SENTIO: A. Hardware Platform for Rapid Prototyping of Wireless Sensor Networks
- 2006
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Ingår i: IECON 2006 - 32ND ANNUAL CONFERENCE ON IEEE INDUSTRIAL ELECTRONICS, VOLS 1-11. - 9781424401352 ; , s. 2243-2247
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Konferensbidrag (refereegranskat)abstract
- A wireless sensor network is always an integrated part of a system. The physical and logical structure of the system, where the sensor network is integrated, are together with other environmental requirements the constraints for the design of the network and the wireless sensor devices. These requirements are very different for different applications and even for one specific system the requirements may be very different on the sensor devices. When developing efficient wireless sensor devices these constraints must be taken into consideration. The diverse constraints we are facing when developing wireless sensor networks and devices for various applications makes it necessary to have a platform-based development approach where as much of the components can be re-used. For the communication, the standard IEEE 802.15.4 provides an excellent platform for developing short range power aware sensor networks. In this paper a hardware platform is presented with its modular structure it provides a highly flexible solution suitable for rapid prototyping of wireless sensors. This platform is a tool for developers of wireless sensor devices as well as system integrators that need to explore different system solutions experimentally
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| 9. |
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| 10. |
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