| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Liu, Shanqiu, et al.
(författare)
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Versatile Stretchable Conductor with Exceptional Resilience and Rapid Rebound Capabilities : Toward Sustainable and Damage-Resistant Soft Electronics
- 2024
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Ingår i: Advanced Functional Materials. - : Wiley-VCH Verlagsgesellschaft. - 1616-301X .- 1616-3028. ; 34:16
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Tidskriftsartikel (refereegranskat)abstract
- Stretchable conductors (SCs) have garnered significant attention in both academia and industry, owing to their promising applications in flexible electronics. However, achieving a highly resilient and rapidly rebounding SC that enables prompt electrical response to applied strain, while also being fully recyclable and self-healable, remains a challenge. Here, the design and preparation of a high-performance SC based on a small bio-molecule (α-Lipoic acid) are reported, using a straightforward, cost-effective, and scalable method. The engineered SC exhibits a ≈100% rebound ratio and fast rebound speed (≈0.4 s) under repeated high strains, along with outstanding electrical sensitivity to both deformation and temperature variation. This ensures that the SC possesses high precision and real-time sensing attributes. Moreover, the SC features high optical transparency (>97%), robust reversible adhesion to diverse surfaces (up to 5.0 MPa), efficient room-temperature self-healing properties, and full recyclability. The superior properties of the SC are fundamentally attributed to the installed hierarchical dynamic bonding at the molecular level, which enables continuous energy dissipation and is analyzed through DFT calculations. The developed SC holds great potential for applications in soft electronics and human–machine interfaces.
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| 3. |
- Liu, Jintao, et al.
(författare)
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Grid parameterization of a conceptual distributed hydrological model through integration of a sub-grid topographic index : necessity and practicability
- 2012
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Ingår i: Hydrological Sciences Journal. - : Informa UK Limited. - 0262-6667 .- 2150-3435. ; 57:2, s. 282-297
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Tidskriftsartikel (refereegranskat)abstract
- Grid-based distributed models have become popular for describing spatial hydrological processes. However, the influence of non-homogeneity within a grid on streamflow simulation was not adequately addressed in the literature. In this study, we investigated how the statistical characteristics of soil moisture storage within a grid impacts on streamflow simulations. The spatial variation of the topographic index, TI, within a grid was used to determine parameter B of the statistical curve of soil moisture storage in the Xinanjiang model. For comparison of influences of the non-homogeneity within a grid on streamflow simulation, two parameterization schemes of soil moisture storage capacity were developed: a grid-parameterization scheme for a distributed model and a catchment-averaged scheme for a semi-distributed model. The practicability and usefulness of the grid-parameterization method were evaluated through model comparisons. The two models were applied in Jiangwan experimental catchment Zhejiang Province, China. Streamflow discharge data at the catchment outlet from 1971 to 1986 at different temporal resolutions, e. g. 15 min and daily time step, were used for model calibration and validation. Statistical results for different grid scales demonstrated that the mean and variation of TI and B decline significantly as the grid scale increases. The simulated streamflow discharges of the two models were similar and the semi-distributed model outperformed the distributed model slightly when the streamflow at the outlet of the catchment was used as the only basis for comparison. In addition, a relatively larger bias in the predicted discharges between these two models was observed along with an abrupt increase of soil moisture saturation ratio. A further analysis of the simulated soil moisture content distribution revealed that the distributed model can provide a reasonable representation of the variable source area concept, which was justified to some extent by the field experiment data.
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| 4. |
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| 5. |
- Nie, Jintao, et al.
(författare)
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SignFind: A Synchronized Sign Language and Chinese Character Teaching Game for Chinese Deaf Children Using Gesture Recognition
- 2022
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Ingår i: CHI EA '22: Extended Abstracts of the 2022 CHI Conference on Human Factors in Computing Systems. - New York, NY, USA : Association for Computing Machinery (ACM). - 9781450391566 ; , s. 1-7
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Konferensbidrag (refereegranskat)abstract
- This paper presents a targeted system to help Chinese deaf children learn both sign language and Chinese characters in early language learning. The system combines sign recognition and in-air writing techniques with games so that children can practice sign language and Chinese character writing at the same time. Sign recognition is used to assess the accuracy of sign language and in-air writing records the process of writing Chinese characters. In addition, the game adds to the learning fun and makes children more willing to complete learning tasks. We have developed a prototype to evaluate the effectiveness of a simultaneous Chinese sign language and Chinese character teaching system based on gesture recognition and in-air writing. We expect that this system will increase children's willingness and efficiency to learn sign language and Chinese characters, and eventually be used to assist in early language education for deaf children in the Chinese region.
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