| 1. |
- Meng, Qinglai, et al.
(författare)
-
Rapid personalized AMR diagnostics using two-dimensional antibiotic resistance profiling strategy employing a thermometric NDM-1 biosensor
- 2021
-
Ingår i: Biosensors and Bioelectronics. - : Elsevier BV. - 0956-5663. ; 193
-
Tidskriftsartikel (refereegranskat)abstract
- Antimicrobial resistance (AMR) threatens global public health and modern surgical medicine. Expression of β-lactamase genes is the major mechanism by which pathogens become antibiotic resistant. Pathogens expressing extended spectrum β-lactamases (ESBL) and carbapenemases (CP) are especially difficult to treat and are associated with increased hospitalization and mortality rates. Despite considerable effort, identification of ESBLs and CPs in a clinically relevant timeframe remains challenging. In this study, a two-dimensional AMR profiling assay strategy was developed employing panels of antibiotics (penicillins, cephamycins, oximino-cephalosporins and carbapenems) and β-lactamases inhibitors (avibactam and EDTA). The assay required the development of a novel biosensor that employed New Delhi metallo-β-lactamase-1 (NDM-1) as the sensing element. Functionally probing β-lactamase activity using substrates and inhibitors combinatorically increased the informational content that enabled the development of assays capable of simultaneous, differential identification of multiple β-lactamases expressed in a single bacterial isolate. More specifically, the assay enabled the simultaneous identification of ESBL and CP in mock samples, as well as in an engineered construct which co-expressed these β-lactamases. The NDM-1 biosensor assay was 16 times and 8 times more sensitive than the ESBL Nordmann/Dortet/Poirel (NDP) and Carba Nordmann/Poirel (NP) assays, respectively. In a retrospective study, NDM-1 biosensor assays were able to differentially identify ESBLs, metallo-CPs and serine-CPs β-lactamases in 23 clinical isolates with 100% accuracy. An assay algorithm was developed which accelerated data analytics reducing turnaround to <1 h. The assay strategy integrated with AI-based data analytics has the potential to provide physicians with a comprehensive readout of patient AMR status.
|
|
| 2. |
- Kabanshi, Alan, et al.
(författare)
-
Potential adaptive behaviour to counteract thermal discomfort in spaces with displacement ventilation or underfloor air distribution systems
- 2016
-
Ingår i: Proceedings of the 14th international conference of Indoor Air Quality and Climate. - Ghent.
-
Konferensbidrag (refereegranskat)abstract
- Building occupants behave in various adaptive ways to restore thermal comfort when in a state of thermal discomfort. These adaptive actions affect building energy use and indoor environmental quality. This paper reports part of a draft risk study, here we focus on potential adaptive behaviour to counteract discomfort in rooms with displacement ventilation (DV) and underfloor air distribution (UFAD) systems. The most likely adaptive behaviours to be taken are: adjust clothing, open/close windows, adjust thermostat and change workstation. No conclusive relationship was found on whether these behaviours are influenced by overall or ankle thermal sensation. Females stated more frequently than males that they would open/close windows, while more males expressed the intention to use heaters and complain to building managers.
|
|
| 3. |
- Liu, Shichao, et al.
(författare)
-
Predicted Percentage Dissatisfied with Ankle Draft
- 2017
-
Ingår i: Indoor Air. - : Hindawi Limited. - 0905-6947 .- 1600-0668. ; 27:4, s. 852-862
-
Tidskriftsartikel (refereegranskat)abstract
- Draft is unwanted local convective cooling. The draft risk model of Fanger et al. (Energy and Buildings 12, 21-39, 1988) estimates the percentage of people dissatisfied with air movement due to overcooling at the neck. There is no model for predicting draft at ankles, which is more relevant to stratified air distribution systems such as underfloor air distribution (UFAD) and displacement ventilation (DV). We developed a model for predicted percentage dissatisfied with ankle draft (PPDAD ) based on laboratory experiments with 110 college students. We assessed the effect on ankle draft of various combinations of air speed (nominal range: 0.1-0.6 m/s), temperature (nominal range: 16.5-22.5 °C), turbulence intensity (at ankles), sex, and clothing insulation (< 0.7 clo; lower legs uncovered and covered). The results show that whole body thermal sensation and air speed at ankles are the dominant parameters affecting draft. The seated subjects accepted a vertical temperature difference of up to 8 °C between ankles (0.1 m) and head (1.1 m) at neutral whole body thermal sensation, 5 °C more than the maximum difference recommended in existing standards. The developed ankle draft model can be implemented in thermal comfort and air diffuser testing standards.
|
|
| 4. |
- Liu, Shichao, et al.
(författare)
-
一种β-内酰胺类抗生素的酶热检测方法
- 2019
-
Ingår i: Journal of Shanxi University (Natural Science Edition). ; :2021-02
-
Tidskriftsartikel (refereegranskat)abstract
- The penicillinase thermistor biosensor（Penicillinase sensor） was developed for the rapid monitoring of blood penem antibiotics concentration and rapid identification of extraneous penicillinase in milk on site.However, the wide application of the penicillinase thermistor biosensor was limited due to its intrinsic poor activity to hydrolyze cephem and carbapenem antibiotics.The recently identified carbapenemase New Delhi metallo-beta-lactamase 1（NDM-1） is able to hydrolyze all commercially available β-lactam antibiotics in high efficacy.We coupled the NDM-1 and the enzymatic thermistor biosensor to develop a NDM-1 thermistor biosensor（NDM-1 sensor） by the installment of the enzymatic thermistor with an enzyme column loaded with NDM-1 conjugated CPG beads.The NDM-1 sensor shows high response activity to Piperacillin（PIP）,Ceftriaxone（CTRX）, and Meropenem（MEM）, and the response activity of the NDM-1 sensor to these three β-lactam antibiotics are all Zn2+ dependent.Moreover, the response activity of the NDM-1 sensor to Penicillin G（P）, PIP, Cefazolin（CZO）, CTRX, Cefepime（FEP） and MEM all linearly correlated with antibiotic concentration from 31.25 to 1 000 mg/L.Within pH from 6.0 to 8.0, the optimal response activity of the NDM-1 sensor to P,PIP, CZO, CTRX and FEP are found at pH 6.5, while the optimal response activity of the NDM-1 sensor to MEM is found at pH8.0.These data indicate that the featured activity of NDM-1 was well maintained after conjugation on CPG beads, and NDM-1 sensor is capable to quantitate three classes of β-lactam antibiotics including penem, cephem and carbapenem within a wide concentration range.
|
|
| 5. |
- Mueller, Sven Christian, et al.
(författare)
-
Interfacing Power System and ICT Simulators : Challenges, State-of-the-Art, and Case Studies
- 2018
-
Ingår i: IEEE Transactions on Smart Grid. - : Institute of Electrical and Electronics Engineers (IEEE). - 1949-3053 .- 1949-3061. ; 9:1, s. 14-24
-
Tidskriftsartikel (refereegranskat)abstract
- With the transition toward a smart grid, the power system has become strongly intertwined with the information and communication technology (ICT) infrastructure. The interdependency of both domains requires a combined analysis of physical and ICT processes, but simulating these together is a major challenge due to the fundamentally different modeling and simulation concepts. After outlining these challenges, such as time synchronization and event handling, this paper presents an overview of state-of-the-art solutions to interface power system and ICT simulators. Due to their prominence in recent research, a special focus is set on co-simulation approaches and their challenges and potentials. Further, two case studies analyzing the impact of ICT on applications in power system operation illustrate the necessity of a holistic approach and show the capabilities of state-of-the-art co-simulation platforms.
|
|
| 6. |
- Schunk, Stefan J., et al.
(författare)
-
Genetically determined NLRP3 inflammasome activation associates with systemic inflammation and cardiovascular mortality
- 2021
-
Ingår i: European Heart Journal. - : Oxford University Press. - 0195-668X .- 1522-9645. ; 42:18, s. 1742-1756
-
Tidskriftsartikel (refereegranskat)abstract
- AimsInflammation plays an important role in cardiovascular disease (CVD) development. The NOD-like receptor protein-3 (NLRP3) inflammasome contributes to the development of atherosclerosis in animal models. Components of the NLRP3 inflammasome pathway such as interleukin-1β can therapeutically be targeted. Associations of genetically determined inflammasome-mediated systemic inflammation with CVD and mortality in humans are unknown.Methods and resultsWe explored the association of genetic NLRP3 variants with prevalent CVD and cardiovascular mortality in 538 167 subjects on the individual participant level in an explorative gene-centric approach without performing multiple testing. Functional relevance of single-nucleotide polymorphisms on NLRP3 inflammasome activation has been evaluated in monocyte-enriched peripheral blood mononuclear cells (PBMCs). Genetic analyses identified the highly prevalent (minor allele frequency 39.9%) intronic NLRP3 variant rs10754555 to affect NLRP3 gene expression. rs10754555 carriers showed significantly higher C-reactive protein and serum amyloid A plasma levels. Carriers of the G allele showed higher NLRP3 inflammasome activation in isolated human PBMCs. In carriers of the rs10754555 variant, the prevalence of coronary artery disease was significantly higher as compared to non-carriers with a significant interaction between rs10754555 and age. Importantly, rs10754555 carriers had significantly higher risk for cardiovascular mortality during follow-up. Inflammasome inducers (e.g. urate, triglycerides, apolipoprotein C3) modulated the association between rs10754555 and mortality.ConclusionThe NLRP3 intronic variant rs10754555 is associated with increased systemic inflammation, inflammasome activation, prevalent coronary artery disease, and mortality. This study provides evidence for a substantial role of genetically driven systemic inflammation in CVD and highlights the NLRP3 inflammasome as a therapeutic target.
|
|
