| 1. |
- Majid, Abbasi, et al.
(författare)
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miR-124-3p Suppresses the Invasiveness and Metastasis of Hepatocarcinoma Cells via Targeting CRKL
- 2020
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Ingår i: Frontiers in Molecular Biosciences. - : Frontiers Media SA. - 2296-889X .- 2296-889X. ; 7
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Tidskriftsartikel (refereegranskat)abstract
- Abnormal expressions of microRNAs are involved in growth and progression of human cancers including hepatocellular carcinoma (HCC). An adaptor protein CRKL plays a pivotal role in HCC growth, whereas miR-124-3p downregulation is associated with clinical stage and the poor survival of patients. However, the relationship between miR-124-3p and CRKL and the molecular mechanisms through which they regulate HCC metastasis remains unclear. In the current work, we explored miR-124-3p and its correlation with CRKL expression in HCC patient tissues. We found that miR-124-3p deficiency is inversely co-related with CRKL overexpression in tumorous tissues of HCC patients, which was also consistent in HCCLM3 and Huh7 HCC cell lines. Target validation data shows that miR-124-3p directly targets CRKL. The overexpression of miR-124-3p reverses the CRKL expression at both mRNA and protein levels and inhibits the cell development, migration, and invasion. Mechanistic investigations showed that CRKL downregulation suppresses the ERK pathway and EMT process, and concomitant decrease in invasion and metastasis of HCC cells. The expressions of key molecules in the ERK pathway such as RAF, MEK, ERK1/2, and pERK1/2 and key promoters of EMT such as N-cadherin and vimentin were downregulated, whereas E-cadherin, a key suppression indicator of EMT, was upregulated. MiR-124-3p-mediated CRKL suppression led to BAX/BCL-2 increase and C-JUN downregulation, which inhibited the cell proliferation and promoted the apoptosis in HCC cells. Collectively, our data illustrates that miR-124-3p acts as an important tumor-suppressive miRNA to suppress HCC carcinogenesis through targeting CRKL. The miR-124-3p-CRKL axial regulated pathway may offer valuable indications for cancer research, diagnosis, and treatment.
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| 2. |
- Xu, Chi, et al.
(författare)
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Macroecological factors explain large-scale spatial population patterns of ancient agriculturalists
- 2015
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Ingår i: Global Ecology and Biogeography. - : Wiley. - 0960-7447 .- 1466-822X. ; 24:9, s. 1030-1039
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Tidskriftsartikel (refereegranskat)abstract
- Aim: It has been well demonstrated that the large-scale distribution patterns of numerous species are driven by similar macroecological factors. However, understanding of this topic remains limited when applied to our own species. Here we take a large-scale look at ancient agriculturalist populations over the past two millennia. The main aim of this study was to test the hypothesis that the patterns of agriculturalist populations were shaped by relevant macroecological factors.Location: China.Methods: Using detailed historical census data, we reconstructed spatial patterns of human population density over 13 imperial dynasties in ancient China, which was dominated by agrarian societies. We used simultaneous autoregressive models to examine the population densities of agriculturalists in relation to climatic, topographic, edaphic and hydrological variables, together with the spatial structure of a concentration of population toward national capitals. The pure and shared effects of these variables and the population-concentration structure were decomposed using a variation partitioning procedure.Results: Spatial population patterns of ancient agriculturalists can be well modelled by climate, topography, soil properties and local hydrological systems. A plausible explanation is that by influencing crop yield these environmental factors essentially drive the distribution of agriculturalists. The population-concentration structure can also explain agriculturalist patterns to a considerable extent. This structure and those environmental factors have largely shared effects in simultaneously shaping these agriculturalist patterns.Main conclusions: While humans can effectively temper environmental constraints at small spatial scales, our results demonstrate that macroecological factors underpin the spatial patterns of humans at large scales. Macroecological constraints and their relative importance are found to be similar for humans and other species, suggesting that similar mechanisms are likely to underlie these macroecological patterns. Our findings have potential implications for the assessment of future responses of humans to global environmental changes.
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| 3. |
- Xu, Tianhua, et al.
(författare)
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Phase Noise Cancellation in Coherent Communication Systems Using a Radio Frequency Pilot Tone
- 2019
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Ingår i: Applied Sciences. - : MDPI. - 2076-3417. ; 9:21
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Tidskriftsartikel (refereegranskat)abstract
- Long-haul optical fiber communication employing digital signal processing (DSP)-based dispersion compensation can be distorted by the phenomenon of equalization-enhanced phase noise (EEPN), due to the reciprocities between the dispersion compensation unit and the local oscillator (LO) laser phase noise (LPN). The impact of EEPN scales increases with the increase of the fiber dispersion, laser linewidths, symbol rates, signal bandwidths, and the order of modulation formats. In this work, the phase noise cancellation (PNC) employing a radio frequency (RF) pilot tone in coherent optical transmission systems has been investigated. A 28-Gsym/s QPSK optical transmission system with a significant EEPN has been implemented, where the carrier phase recovery (CPR) was realized using the one-tap normalized least-mean-square (NLMS) estimation and the differential phase detection (DPD), respectively. It is shown that the RF pilot tone can entirely eliminate the LPN and efficiently suppress the EEPN when it is applied prior to the CPR.
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| 4. |
- Yuan, Wenping, et al.
(författare)
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Differentiating moss from higher plants is critical in studying the carbon cycle of the boreal biome.
- 2014
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Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 5
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Tidskriftsartikel (refereegranskat)abstract
- The satellite-derived normalized difference vegetation index (NDVI), which is used for estimating gross primary production (GPP), often includes contributions from both mosses and vascular plants in boreal ecosystems. For the same NDVI, moss can generate only about one-third of the GPP that vascular plants can because of its much lower photosynthetic capacity. Here, based on eddy covariance measurements, we show that the difference in photosynthetic capacity between these two plant functional types has never been explicitly included when estimating regional GPP in the boreal region, resulting in a substantial overestimation. The magnitude of this overestimation could have important implications regarding a change from a current carbon sink to a carbon source in the boreal region. Moss abundance, associated with ecosystem disturbances, needs to be mapped and incorporated into GPP estimates in order to adequately assess the role of the boreal region in the global carbon cycle.
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