| 1. |
- Gui, Xinru, et al.
(författare)
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Identification and validation of volatile organic compounds in bile for differential diagnosis of perihilar cholangiocarcinoma
- 2023
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Ingår i: Clinica Chimica Acta. - : Elsevier. - 0009-8981 .- 1873-3492. ; 541
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Tidskriftsartikel (refereegranskat)abstract
- Early and differential diagnosis of perihilar cholangiocarcinoma (PHCCA) is highly challenging. This study aimed to evaluate whether volatile organic compounds (VOCs) in bile samples could be emerging diagnostic biomarkers for PHCCA. We collected 200 bile samples from patients with PHCCA and benign biliary diseases (BBD), including a 140-patient training cohort and an 60-patient test cohort. Gas chromatography-ion mobility spec-trometry (GC-IMS) was used for VOCs detection. The predictive models were constructed using machine learning algorithms. Our analysis detected 19 VOC substances using GC-IMS in the bile samples and resulted in the identification of three new VOCs, 2-methoxyfuran, propyl isovalerate, and diethyl malonate that were found in bile. Unsupervised hierarchical clustering analysis supported that VOCs detected in the bile could distinguish PHCCA from BBD. Twelve VOCs defined according to 32 signal peaks had significant statistical significance between BBD and PHCCA, including four up-regulated VOCs in PHCCA, such as 2-ethyl-1-hexanol, propyl iso-valerate, cyclohexanone, and acetophenone, while the rest eight VOCs were down-regulated. ROC curve analysis revealed that machine learning models based on VOCs could help diagnosing PHCCA. Among them, SVM pro-vided the highest AUC of 0.966, with a sensitivity and specificity of 93.1% and 100%, respectively. The diag-nostic model based on different VOC spectra could be a feasible method for the differential diagnosis of PHCCA.
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| 2. |
- Chen, Shan, et al.
(författare)
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An efficient enzymatic aminolysis for kinetic resolution of aromatic alpha-hydroxyl acid in non-aqueous media
- 2016
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Ingår i: Tetrahedron Letters. - : Elsevier. - 0040-4039 .- 1359-8562. ; 57:48, s. 5312-5314
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Tidskriftsartikel (refereegranskat)abstract
- A new and highly efficient enzymatic aminolysis approach for kinetic resolution of aromatic a-hydroxy acid in non-aqueous media has been developed. The corresponding alpha-hydroxyl acid ester was employed as the substrate, and commercially available Candida antarctica lipase B is used as the biocatalyst, anhydrous ammonia is the resolving agent. Reactions can be proceeded smoothly in organic solvent at ambient temperatures. High concentration of substrate is allowed due to the application of organic media and the products are obtained in yields of up to 49% with ee values of up to 99%, and with E value of >300, representing an appealing and promising protocol for large-scale preparations.
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| 3. |
- Liu, Yiwei, et al.
(författare)
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Effect of the burner arrangement on Slab reheating characteristics in a reheating furnace
- 2017
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Ingår i: Chemical Engineering Transactions. - 1974-9791. ; 61, s. 97-102
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Tidskriftsartikel (refereegranskat)abstract
- Slab reheating characteristics in the walking beam type reheating furnace were studied by using the finite volume method (FVM). A geometry model for simulating the gas circulation, heat transfer and slabs reheating process in a reheating furnace was developed by using ANSYS FLUENT software. Usually, slabs are heated in the furnace, and temperature values of the slabs reach above 1,500 K. After that, they are transported to the rolling mill. The slab reheating in a reheating furnace is influenced by many factors, such as gas velocity, moving speed of the slabs, and burner arrangement. Especially, the arrangement of burners shows a great effect on reheating the slabs. In the paper, the main object of study is to investigate the temperature distribution in the reheating furnace by considering the effect of the burner position. In addition, it is found that the temperature fields of the slabs are significantly affected by changing the positions of the burners.
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| 4. |
- Liu, Yiwei, et al.
(författare)
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Performance of fuel-air combustion in a reheating furnace at different flowrate and inlet conditions
- 2020
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Ingår i: Energy. - : Elsevier BV. - 0360-5442. ; 206
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Tidskriftsartikel (refereegranskat)abstract
- A geometry model for simulating the gas circulation, heat transfer and slab heating process in a reheating furnace was developed with numerical simulation. The slabs are heated in the furnace and the temperature of the slabs become above more than 1500 K, and then they are transported to the rolling mill. The slab heating in a reheating furnace is influenced by many factors, such as gas velocity, the movement speed of the slab, the height and spacing of the burners and others. The slab heating characteristics in the walking beam type reheating furnace were studied by considering the slab movement. The movement of the slabs was processed by transferring temperature data from the previous location to the next location. It was assumed that no time is spent for moving the slabs. Especially, the effects of different inlet boundaries of the burners were investigated. Results show that the entrainment effect from the inner air flow results in a larger coverage area of the flames, and there is an increase of more than 28.5% in the slab temperature over the whole furnace.
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| 5. |
- Rath, Emma M, et al.
(författare)
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BAMLET kills chemotherapy-resistant mesothelioma cells, holding oleic acid in an activated cytotoxic state
- 2018
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 13:8
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Tidskriftsartikel (refereegranskat)abstract
- Malignant pleural mesothelioma is an aggressive cancer with poor prognosis. Here we have investigated in vitro efficacy of BAMLET and BLAGLET complexes (anti-cancer complexes consisting of oleic acid and bovine α-lactalbumin or β-lactoglobulin respectively) in killing mesothelioma cells, determined BAMLET and BLAGLET structures, and investigated possible biological mechanisms. We performed cell viability assays on 16 mesothelioma cell lines. BAMLET and BLAGLET having increasing oleic acid content inhibited human and rat mesothelioma cell line proliferation at decreasing doses. Most of the non-cancer primary human fibroblasts were more resistant to BAMLET than were human mesothelioma cells. BAMLET showed similar cytotoxicity to cisplatin-resistant, pemetrexed-resistant, vinorelbine-resistant, and parental rat mesothelioma cells, indicating the BAMLET anti-cancer mechanism may be different to drugs currently used to treat mesothelioma. Cisplatin, pemetrexed, gemcitabine, vinorelbine, and BAMLET, did not demonstrate a therapeutic window for mesothelioma compared with immortalised non-cancer mesothelial cells. We demonstrated by quantitative PCR that ATP synthase is downregulated in mesothelioma cells in response to regular dosing with BAMLET. We sought structural insight for BAMLET and BLAGLET activity by performing small angle X-ray scattering, circular dichroism, and scanning electron microscopy. Our results indicate the structural mechanism by which BAMLET and BLAGLET achieve increased cytotoxicity by holding increasing amounts of oleic acid in an active cytotoxic state encapsulated in increasingly unfolded protein. Our structural studies revealed similarity in the molecular structure of the protein components of these two complexes and in their encapsulation of the fatty acid, and differences in the microscopic structure and structural stability. BAMLET forms rounded aggregates and BLAGLET forms long fibre-like aggregates whose aggregation is more stable than that of BAMLET due to intermolecular disulphide bonds. The results reported here indicate that BAMLET and BLAGLET may be effective second-line treatment options for mesothelioma.
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| 6. |
- Wang, Jin, et al.
(författare)
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Analysis of slab heating characteristics in a reheating furnace
- 2017
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Ingår i: Energy Conversion and Management. - : Elsevier BV. - 0196-8904. ; 149, s. 928-936
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Tidskriftsartikel (refereegranskat)abstract
- For a reheating furnace, an analysis of thermal efficiency depends on precise investigations of the combustion and flow characteristics inside a furnace. Especially, the flow field of the hot gas has significant influence on heating slabs. The slabs are heated up to the temperature over 1500. K, and then they are transported to the rolling mill. The heating efficiency is affected by many factors, such as fuel type, locations of both slabs and burners, thermal properties of slabs, geometry of slab supporting systems, and so on. In the paper, some efforts were made to simulate the thermo-fluid mechanical phenomenon inside the furnace. The slab heating characteristics in the reheating furnace were investigated by using the finite-volume method (FVM). The unsteady calculation was performed to obtain the temperature distribution by considering the movement of the slabs in the reheating furnace. To treat radiation emitted by the walls and the gas, numerical simulations were completed by employing ANSYS FLUENT. The configurations of skid posts and beams were also considered to evaluate the effect of the burner position. Results indicate that the case with 6 side burners has a higher heating efficiency both in the heating and soaking sections.
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| 7. |
- Xin, Yiwei, et al.
(författare)
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Fusobacterium nucleatum- induced exosomal HOTTIP promotes gastric cancer progression through the microRNA-885-3p/EphB2 axis
- 2023
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Ingår i: Cancer Science. - : John Wiley & Sons. - 1347-9032 .- 1349-7006. ; 114:6, s. 2360-2374
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Tidskriftsartikel (refereegranskat)abstract
- Recent studies have reported that Fusobacterium nucleatum (Fn) is associated with gastric cancer (GC). Cancer-derived exosomes contain key regulatory noncoding RNAs and are a crucial medium of intercellular communication. However, the function and regulatory mechanism of exosomes (Fn-GCEx) secreted from Fn-infected GC cells remains unclear. In this study, Fn-GCEx enhanced the proliferation, migration, and invasion capacity of GC cells in vitro, as well as tumor growth and metastasis in vivo. HOTTIP was also upregulated in GC cells treated with Fn-GCEx. Moreover, knockdown of HOTTIP weakened the effects of Fn-GCEx in recipient GC cells. Mechanistically, HOTTIP promoted EphB2 expression by sponging microRNA (miR)-885-3p, thus activating the PI3K/AKT pathway in Fn-GCEx treated GC cells. Overall, Fn infection induced the upregulation of exosomal HOTTIP from GC cells that subsequently promoted GC progression through the miR-885-3p/EphB2/PI3K/AKT axis. Herein, we identify a potential molecular pathway and therapeutic target for GC.
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