| 1. |
|
|
| 2. |
- Eklund, D. Magnus, et al.
(författare)
-
Homologues of the Arabidopsis thaliana SHI/STY/LRP1 genes control auxin biosynthesis and affect growth and development in the moss Physcomitrella patens
- 2010
-
Ingår i: Development. - : The Company of Biologists. - 0950-1991 .- 1477-9129. ; 137:8, s. 1275-1284
-
Tidskriftsartikel (refereegranskat)abstract
- The plant hormone auxin plays fundamental roles in vascular plants. Although exogenous auxin also stimulates developmental transitions and growth in non-vascular plants, the effects of manipulating endogenous auxin levels have thus far not been reported. Here, we have altered the levels and sites of auxin production and accumulation in the moss Physcomitrella patens by changing the expression level of homologues of the Arabidopsis SHI/STY family proteins, which are positive regulators of auxin biosynthesis genes. Constitutive expression of PpSHI1 resulted in elevated auxin levels, increased and ectopic expression of the auxin response reporter GmGH3pro:GUS, and in an increased caulonema/chloronema ratio, an effect also induced by exogenous auxin application. In addition, we observed premature ageing and necrosis in cells ectopically expressing PpSHI1. Knockout of either of the two PpSHI genes resulted in reduced auxin levels and auxin biosynthesis rates in leafy shoots, reduced internode elongation, delayed ageing, a decreased caulonema/chloronema ratio and an increased number of axillary hairs, which constitute potential auxin biosynthesis sites. Some of the identified auxin functions appear to be analogous in vascular and non-vascular plants. Furthermore, the spatiotemporal expression of the PpSHI genes and GmGH3pro:GUS strongly overlap, suggesting that local auxin biosynthesis is important for the regulation of auxin peak formation in non-vascular plants.
|
|
| 3. |
- Izadi, Zara, et al.
(författare)
-
Environmental and societal factors associated with COVID-19-related death in people with rheumatic disease : an observational study
- 2022
-
Ingår i: The Lancet Rheumatology. - : Elsevier. - 2665-9913. ; 4:9, s. e603-e613
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Differences in the distribution of individual-level clinical risk factors across regions do not fully explain the observed global disparities in COVID-19 outcomes. We aimed to investigate the associations between environmental and societal factors and country-level variations in mortality attributed to COVID-19 among people with rheumatic disease globally.Methods: In this observational study, we derived individual-level data on adults (aged 18–99 years) with rheumatic disease and a confirmed status of their highest COVID-19 severity level from the COVID-19 Global Rheumatology Alliance (GRA) registry, collected between March 12, 2020, and Aug 27, 2021. Environmental and societal factors were obtained from publicly available sources. The primary endpoint was mortality attributed to COVID-19. We used a multivariable logistic regression to evaluate independent associations between environmental and societal factors and death, after controlling for individual-level risk factors. We used a series of nested mixed-effects models to establish whether environmental and societal factors sufficiently explained country-level variations in death.Findings: 14 044 patients from 23 countries were included in the analyses. 10 178 (72·5%) individuals were female and 3866 (27·5%) were male, with a mean age of 54·4 years (SD 15·6). Air pollution (odds ratio 1·10 per 10 μg/m3 [95% CI 1·01–1·17]; p=0·0105), proportion of the population aged 65 years or older (1·19 per 1% increase [1·10–1·30]; p<0·0001), and population mobility (1·03 per 1% increase in number of visits to grocery and pharmacy stores [1·02–1·05]; p<0·0001 and 1·02 per 1% increase in number of visits to workplaces [1·00–1·03]; p=0·032) were independently associated with higher odds of mortality. Number of hospital beds (0·94 per 1-unit increase per 1000 people [0·88–1·00]; p=0·046), human development index (0·65 per 0·1-unit increase [0·44–0·96]; p=0·032), government response stringency (0·83 per 10-unit increase in containment index [0·74–0·93]; p=0·0018), as well as follow-up time (0·78 per month [0·69–0·88]; p<0·0001) were independently associated with lower odds of mortality. These factors sufficiently explained country-level variations in death attributable to COVID-19 (intraclass correlation coefficient 1·2% [0·1–9·5]; p=0·14).Interpretation: Our findings highlight the importance of environmental and societal factors as potential explanations of the observed regional disparities in COVID-19 outcomes among people with rheumatic disease and lay foundation for a new research agenda to address these disparities.
|
|
| 4. |
- Khorram-Manesh, Amir, 1958, et al.
(författare)
-
Emergency Management and Preparedness Training for Youth (EMPTY) : The Results of the First Swedish Pilot Study
- 2018
-
Ingår i: Disaster Medicine and Public Health Preparedness. - : Cambridge University Press (CUP). - 1935-7893 .- 1938-744X. ; 12:6, s. 685-688
-
Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To examine the impact of a simulation training in raising a group of young students' personal and situational awareness in disasters and emergencies.METHODS: In total, 25 young students participated in two simulation scenarios representing two actual events, fire, and shooting, using a combination of two validated simulation training (Emergency Management and Preparedness Training for Youth [EMPTY]). The changes in their knowledge and awareness were evaluated by using questionnaires and the whole simulation was evaluated by three independent observers and a reference group.RESULTS: New concepts of emergency management, for example, evacuation, and barricading, could be trained in a safe environment. There was a significant increase in students' personal and situational awareness and their active engagement in the management of emergencies.CONCLUSION: EMPTY could raise the youth basic knowledge and ability to understand the concept of preparedness by being mentally prepared, available for collaboration, gaining a higher confidence, understanding the physical and psychological consequences of a major incident and the importance of their own safety. (Disaster Med Public Health Preparedness. 2018; page 1 of 4).
|
|
| 5. |
|
|
| 6. |
- Ljung, Per, et al.
(författare)
-
Detection of titanium in electrothermal atomizers by laser-induced fluorescence : Part 2. Investigation of various types of atomizers
- 1997
-
Ingår i: Spectrochimica Acta Part B - Atomic Spectroscopy. - : Elsevier. - 0584-8547 .- 1873-3565. ; 52:6, s. 703-716
-
Tidskriftsartikel (refereegranskat)abstract
- Different types of electrothermal atomizers (graphite and tungsten furnaces) have been studied for the determination of Ti by laser-induced fluorescence (LIF). It was found that all graphite furnaces suffer from both bulk contamination by Ti, corresponding to tens of pg per firing, and significant memory effects in such a way that the high sensitivity of the LIF technique could not be fully utilized. The detectability of Ti by the LIF technique was limited by fluctuations of the atomizer blank signals and therefore depended mainly on the Ti history of the furnaces. The detection limit of Ti was found to be similar for all graphite furnaces: around 1 pg for furnaces not exposed to any substantial amounts of Ti, and a few pg for those which had been exposed to a few hundreds of pg of Ti (or more). It was found that the transversely heated graphite atomizer (THGA) from Perkin-Elmer gives the most reproducible atomization of Ti.The tungsten furnace showed no memory effects and therefore no empty firings (burn-outs) between determinations are needed. The tungsten furnace, however, afforded significantly weaker signals (approximately 250-fold) than the graphite furnaces; this was attributed to incomplete atomization processes. The Ti signal from the tungsten furnace could be increased by the addition of H2 in the buffer gas. For 20% of H2 added, however, the analyte signal was still weaker by a factor of 8 than that seen in a graphite furnace shielded with Ar (the remaining difference is primarily attributed to fluorescence quenching from H2) In addition, the precision of the measurements was poorer than that obtained from graphite furnaces.
|
|
| 7. |
- Ljung, Per, et al.
(författare)
-
Detection of titanium in electrothermal atomizers by laser-induced fluorescence : Part 1. Determination of optimum excitation and detection wavelengths
- 1997
-
Ingår i: Spectrochimica Acta Part B - Atomic Spectroscopy. - : Elsevier. - 0584-8547 .- 1873-3565. ; 52:6, s. 675-701
-
Tidskriftsartikel (refereegranskat)abstract
- A detailed investigation has been performed of suitable excitation and detection wavelengths of Ti for the technique of laser-induced fluorescence in electrothermal atomizers. Fluorescence spectra (most often in the 250-350 nm region) from the 39 excitation wavelengths conjectured to be the most important (in the 220-270 nm region) have been investigated in more detail by the use of an intensified CCD detector. The fluorescence spectra were found to have a rich occurrence of peaks (about 50 each), of which many cannot be found in existing atomic wavelength data in the literature. All of the peaks found (with one exception) could be identified thanks to a home-made program that calculates atomic wavelengths from existing energy level data. Most of the spectra are dominated by ''indirect'' transitions (i.e. fluorescence from an upper level that is different from the one accessed by the laser) despite the prediction of existing ''direct'' transitions. This indicates that the collisional redistribution processes amongst the excited levels, in general, are faster than typical fluorescence rates for Ti in graphite furnaces. Suitable excitation and detection wavelength combinations are given. One such suitable choice is excitation at 264.662 nm since the fluorescence following this excitation consisted of peaks of almost equal magnitude in three different wavelength regions (around 295, 319 and 338 nm).
|
|
| 8. |
- Manderstedt, Eric, et al.
(författare)
-
Detection of F8 int22h inversions using digital droplet PCR and mile-post assays
- 2020
-
Ingår i: Journal of Thrombosis and Haemostasis. - : Wiley-Blackwell. - 1538-7933 .- 1538-7836. ; 8:5, s. 1039-1049
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Inversions involving intron 22 (Inv22) of F8 are detected in approximately 45% of all severe hemophilia A patients. Diagnosis is complicated by the large size of the ~9.5 kb int22h repeated sequence which generates the inversions. Methods such as long-range PCR and inverse-shifting PCR are currently used diagnostically, but suffer from low PCR efficiencies and are difficult to standardize.OBJECTIVES: To design and validate a sensitive and robust assay for the detection of F8 int22h inversions.METHODS: Digital droplet PCR using mile-post assays was used to investigate archival DNA samples.RESULTS: The detection of linkage as a function of physical distance between loci was investigated using an anchor locus and mile-post loci located at 1, 6, 12 and 15 kb distances from the anchor locus. The proportion of linked molecules decreased with increasing distance between loci and showed 30-40% linked molecules for loci 12-15 kb apart. Mile-post assays specific for wild type and Inv22 type 1 and 2 chromosomes were then designed and optimized. All three assays showed high specificities and sensitivities, with coefficients of variation < 5% for all assays. Analysis of 106 patients and 20 carrier mothers showed complete concordance with previously known mutation status. The analysis demonstrated the robustness of the assays versus input DNA concentration (6 ng and higher) and level of fragmentation.CONCLUSIONS: Digital droplet PCR and mile-post assays can be used to detect F8 int22h inversions. The assay systems are technically simple to perform, highly efficient and robust.
|
|
| 9. |
- Manderstedt, Eric, et al.
(författare)
-
Detection of F8 int22h inversions using digital droplet PCR and mile-post assays
- 2020
-
Ingår i: Journal of Thrombosis and Haemostasis. - : Wiley-Blackwell Publishing Ltd. - 1538-7933 .- 1538-7836. ; 8:5, s. 1039-1049
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Inversions involving intron 22 (Inv22) of F8 are detected in approximately 45% of all severe hemophilia A patients. Diagnosis is complicated by the large size of the ~9.5 kb int22h repeated sequence which generates the inversions. Methods such as long-range PCR and inverse-shifting PCR are currently used diagnostically, but suffer from low PCR efficiencies and are difficult to standardize. OBJECTIVES: To design and validate a sensitive and robust assay for the detection of F8 int22h inversions. METHODS: Digital droplet PCR using mile-post assays was used to investigate archival DNA samples. RESULTS: The detection of linkage as a function of physical distance between loci was investigated using an anchor locus and mile-post loci located at 1, 6, 12 and 15 kb distances from the anchor locus. The proportion of linked molecules decreased with increasing distance between loci and showed 30-40% linked molecules for loci 12-15 kb apart. Mile-post assays specific for wild type and Inv22 type 1 and 2 chromosomes were then designed and optimized. All three assays showed high specificities and sensitivities, with coefficients of variation < 5% for all assays. Analysis of 106 patients and 20 carrier mothers showed complete concordance with previously known mutation status. The analysis demonstrated the robustness of the assays versus input DNA concentration (6 ng and higher) and level of fragmentation. CONCLUSIONS: Digital droplet PCR and mile-post assays can be used to detect F8 int22h inversions. The assay systems are technically simple to perform, highly efficient and robust.
|
|
| 10. |
- Manderstedt, Eric, et al.
(författare)
-
Detection of mosaics in hemophilia A by deep Ion Torrent sequencing and droplet digital PCR
- 2020
-
Ingår i: Research and practice in thrombosis and haemostasis. - : Wiley. - 2475-0379. ; 4:7, s. 1121-1130
-
Tidskriftsartikel (refereegranskat)abstract
- Background: The occurrence of mosaicism in hemophilia A (HA) has been investigated in several studies using different detection methods. Objectives: To characterize and compare the ability of AmpliSeq/Ion Torrent sequencing and droplet digital polymerase chain reaction (ddPCR) for mosaic detection in HA. Methods: Ion Torrent sequencing and ddPCR were used to analyze 20 healthy males and 16 mothers of sporadic HA patients. Results: An error-rate map over all coding positions and all positions reported as mutated in the F8-specific mutation database was produced. The sequencing produced a mean read depth of >1500X where >97% of positions were covered by >100 reads. Higher error frequencies were observed in positions with A or T as reference allele and in positions surrounded on both sides with C or G. Seventeen of 9319 positions had a mean substitution error frequency >1%. The ability to identify low-level mosaicism was determined primarily by read depth and error rate of each specific position. Limit of detection (LOD) was <1% for 97% of positions with substitutions and 90% of indel positions. The positions with LOD >1% require repeated testing and mononucleotide repeats with more than four repeat units need an alternative analysis strategy. Mosaicism was detected in 1 of 16 mothers and confirmed using ddPCR. Conclusions: Deep sequencing using an AmpliSeq/Ion Torrent strategy allows for simultaneous identification of disease-causing mutations in patients and mosaicism in mothers. ddPCR has high sensitivity but is hampered by the need for mutationspecific design.
|
|
