| 1. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 2. |
- Cheng, Yu Chen, et al.
(författare)
-
Supercontinuum generation in a multi-plate medium
- 2016
-
Ingår i: Optics Express. - 1094-4087. ; 24:7, s. 7224-7231
-
Tidskriftsartikel (refereegranskat)abstract
- We analyze femtosecond supercontinuum generation in a distribution of thin solid plates to show that the distributed scheme inhibits processes leading to pulse breakup while allowing spectral expansion to proceed as desired. We introduce basic criteria for setting the plate thickness or initial laser intensity and the location of each plate in the laser beam path and confirm that under these conditions a fully-coherent and intense supercontinuum can be generated for input peak power of as much as two thousand times the critical power for self-focusing of the solid medium.
|
|
| 3. |
- Elsayed, Mohamed Hammad, et al.
(författare)
-
Overcoming small-bandgap charge recombination in visible and NIR-light-driven hydrogen evolution by engineering the polymer photocatalyst structure
- 2024
-
Ingår i: Nature Communications. - 2041-1723. ; 15:1
-
Tidskriftsartikel (refereegranskat)abstract
- Designing an organic polymer photocatalyst for efficient hydrogen evolution with visible and near-infrared (NIR) light activity is still a major challenge. Unlike the common behavior of gradually increasing the charge recombination while shrinking the bandgap, we present here a series of polymer nanoparticles (Pdots) based on ITIC and BTIC units with different π-linkers between the acceptor-donor-acceptor (A-D-A) repeated moieties of the polymer. These polymers act as an efficient single polymer photocatalyst for H2 evolution under both visible and NIR light, without combining or hybridizing with other materials. Importantly, the difluorothiophene (ThF) π-linker facilitates the charge transfer between acceptors of different repeated moieties (A-D-A-(π-Linker)-A-D-A), leading to the enhancement of charge separation between D and A. As a result, the PITIC-ThF Pdots exhibit superior hydrogen evolution rates of 279 µmol/h and 20.5 µmol/h with visible (>420 nm) and NIR (>780 nm) light irradiation, respectively. Furthermore, PITIC-ThF Pdots exhibit a promising apparent quantum yield (AQY) at 700 nm (4.76%).
|
|
| 4. |
- Lu, Chih Hsuan, et al.
(författare)
-
Multi-plate generation and compression of an intense supercontinuum pulse
- 2016
-
Ingår i: High Intensity Lasers and High Field Phenomena, HILAS 2016. - 9781943580095
-
Konferensbidrag (refereegranskat)abstract
- An intense octave-spanning supercontinuum with pulse energy of 160 μJ has been generated in an all-solid-state multiple-plate arrangement and successfully compressed to near-transform limited single-cycle duration of 2.8 fs.
|
|
