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| 2. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
- Gao, Yan, et al.
(författare)
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Perovskite Hydroxide CoSn(OH)(6) Nanocubes for Efficient Photoreduction of CO2 to CO
- 2018
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Ingår i: ACS Sustainable Chemistry and Engineering. - : AMER CHEMICAL SOC. - 2168-0485. ; 6:1, s. 781-786
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Tidskriftsartikel (refereegranskat)abstract
- Perovskite hydroxide CoSn(OH)(6) nanoparticles were synthesized and used for the first time in the photocatalytic reduction of CO2 to CO. Under mild reaction conditions and using [Ru(bpy)(3)](PF6)(2) as the photosensitizer, a high photocatalytic efficiency of 19.3 mu mol for CO evolution with a high selectivity of 86.46% was obtained. The photocatalytic TEOA activity and CO selectivity were further improved by adding weak Bronsted acids, as proton sources, to the system.
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| 4. |
- Wang, Shu Min, 1963, et al.
(författare)
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Phosphorus and Nitrogen Containing Dilute Bismides
- 2019
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Ingår i: Springer Series in Materials Science. - Singapore : Springer Singapore. - 2196-2812 .- 0933-033X. ; , s. 97-123
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Phosphorus and nitrogen containing dilute bismides differ from arsenic and antimony containing bismides in that the anions have large differences in atomic size and electronegativity, offering rich potentials for strain as well as bandgap engineering. In this chapter, we show theoretical modeling, epitaxy and characterizations of III-PBi and III-NBi and their quaternary alloys.
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| 5. |
- Ye, Lu, et al.
(författare)
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Assembly of highly efficient photocatalytic CO2 conversion systems with ultrathin two-dimensional metal-organic framework nanosheets
- 2018
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Ingår i: Applied Catalysis B. - : ELSEVIER SCIENCE BV. - 0926-3373 .- 1873-3883. ; 227, s. 54-60
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Tidskriftsartikel (refereegranskat)abstract
- An ultrathin two-dimensional Zn porphyrin-based metal-organic framework (Zn-MOF nanosheets) is developed and used for the first time in photoreduction of CO2 to CO. Consequently, two novelty noble-metal-free hybrid photocatalytic systems are established and displayed outstanding photocatalytic activity and selectivity for CO evolution under mild photocatalytic reaction conditions. The insight revealed Zn-MOF nanosheets as photo sensitizer displays a better charge transport ability and longer lifetime of the photogenerated electron-hole pairs than the Zn-MOF bulk, which are confirmed by photoelectrochemical impedance and photoluminescence (PL) measurements. These studies show that the development of noble-metal-free photocatalytic systems and various MOF-based materials for photocatalytic applications are promising.
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