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- Danielsson, Frida, 1984-
(författare)
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Integration of RNA and protein expression profiles to study human cells
- 2016
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Cellular life is highly complex. In order to expand our understanding of the workings of human cells, in particular in the context of health and disease, detailed knowledge about the underlying molecular systems is needed. The unifying theme of this thesis concerns the use of data derived from sequencing of RNA, both within the field of transcriptomics itself and as a guide for further studies at the level of protein expression. In paper I, we showed that publicly available RNA-seq datasets are consistent across different studies, requiring only light processing for the data to cluster according to biological, rather than technical characteristics. This suggests that RNA-seq has developed into a reliable and highly reproducible technology, and that the increasing amount of publicly available RNA-seq data constitutes a valuable resource for meta-analyses. In paper II, we explored the ability to extrapolate protein concentrations by the use of RNA expression levels. We showed that mRNA and corresponding steady-state protein concentrations correlate well by introducing a gene-specific RNA-to-protein conversion factor that is stable across various cell types and tissues. The results from this study indicate the utility of RNA-seq also within the field of proteomics.The second part of the thesis starts with a paper in which we used transcriptomics to guide subsequent protein studies of the molecular mechanisms underlying malignant transformation. In paper III, we applied a transcriptomics approach to a cell model for defined steps of malignant transformation, and identified several genes with interesting expression patterns whose corresponding proteins were further analyzed with subcellular spatial resolution. Several of these proteins were further studied in clinical tumor samples, confirming that this cell model provides a relevant system for studying cancer mechanisms. In paper IV, we continued to explore the transcriptional landscape in the same cell model under moderate hypoxic conditions.To conclude, this thesis demonstrates the usefulness of RNA-seq data, from a transcriptomics perspective and beyond; to guide in analyses of protein expression, with the ultimate goal to unravel the complexity of the human cell, from a holistic point of view.
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| 2. |
- Lundberg, Emma, 1984, et al.
(författare)
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Deconstructing spiritual care: Discursive underpinnings within palliative care research
- 2024
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Ingår i: Nursing Inquiry. - 1320-7881. ; 31:3
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Tidskriftsartikel (refereegranskat)abstract
- Religion and spirituality are integral to the philosophy of palliative care, shaping its approach to spiritual care. This article aims to examine the discourses within palliative care research to illuminate prevailing assumptions regarding spiritual care. Eighteen original articles were analyzed to examine how spiritual care is understood within palliative care. The analysis, informed by Foucault, aimed to identify recurring discourses. The finding reveals that, in palliative care research, spirituality is viewed as enigmatic yet inherently human and natural, assuming that every individual has a spiritual dimension. The analysis points to healthcare professionals being expected to hold certain qualities to put spiritual care into practice. The analysis also reveals that in the analyzed articles, the concept of spiritual care is rooted in a Christian context, with the belief that all individuals possess inherent spirituality or religiosity, a concept often associated with Christian theology. The included articles often utilize theological terms and emphasize a monotheistic viewpoint. Spirituality is articulated as a complex, distinct concept, challenging clear definitions and professional responsibilities. Further, a moral formation of healthcare professionals is described, interpelling and ascribing qualities that healthcare professionals need to provide spiritual care.
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| 5. |
- Lundberg, Emma, 1984, et al.
(författare)
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Place of death among foreign-born individuals: a national population-based register study.
- 2023
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Ingår i: Palliative care and social practice. - 2632-3524. ; 17
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Tidskriftsartikel (refereegranskat)abstract
- Relatively little is known about where foreign-born individuals die in Sweden and how birth region might influence place of death. Thus, there is a need for population-based studies investigating place of death and associated factors among foreign-born individuals.The aim of this study was to identify variations in place of death among foreign-born individuals residing in Sweden and to compare place of death between the foreign- and domestic-born population. We also examine the association between place of death, underlying cause of death and sociodemographic characteristics among the foreign-born population.A population-based register study.All deceased individuals ⩾18years of age in Sweden with a registered place of death between 2012 and 2019 (n=682,697). Among these, 78,466 individuals were foreign-born. Univariable multinomial logistic regression modelling and multivariable multinomial logistic regression analyses were performed.Overall, hospital was the most common place of death among the foreign-born population. However, there were variations in place of death related to region of birth. Compared to domestic-born, a higher proportion of foreign-born individuals dies at home, the majority of whom were born on the African continent.Region of birth is one of the several factors associated with place of death among foreign-born individuals. Further research is needed to explore both preferences and barriers to place of death among foreign-born individuals.
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| 10. |
- Lundmark, Sonja, 1966, et al.
(författare)
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Experiences from a distance course in electric drives including on-line labs and tutorials
- 2012
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Ingår i: 2012 20th International Conference on Electrical Machines, ICEM 2012, Marseille, 2 September - 5 September 2012. - 9781467301428 ; , s. 3050-3055
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Konferensbidrag (refereegranskat)abstract
- This paper explains the steps taken to convert the electric drives course activities at Chalmers University of Technology to a distance course directed towards the automotive industry, including interactive remote web-labs, and on-line tutorials and computer lab sessions. A first pilot course is conducted during autumn 2011. From the course evaluation, from student results and from experience of the involved teachers during the work, it could be concluded that the first pilot course worked as planned, yielding good results in students' performance compared to the on-campus course, except for the practical lab part where students from both courses performed equally well. The web course got an average of 4.2 (out of 5.0) from the course evaluations. In order to improve the web-course, the interactivity during the practical labs should be improved.
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