| 1. |
- Chalkias, Helena, et al.
(författare)
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Barcelona 2009 – inte bara fotboll
- 2009
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Ingår i: Svensk gris med knorr. - 1650-5077. ; , s. 30, 32-
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 2. |
- Hawamdeh, Shurouq, 1982, et al.
(författare)
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Midwives’ attitude towards labour pain: Expectations and perceptions by Jordanian women
- 2016
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Ingår i: NJF congress, May 2016, Sweden..
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Background: In Jordan, as in other countries, the majority of women give birth in public hospitals where the midwives are the main birth attendants. Although most women have trust in their midwives; there are studies describing dissatisfaction of childbirth experience, fears associated with experiencing painful births and ineffective pain management during childbirth. Thus, there is a need to understand the Jordanian midwives' attitude towards labour pain from both the midwives and the women’s perspectives. Aim: To explore the Jordanian midwives' attitude towards labour pain by assessing the midwives' knowledge and attitude towards pain and the women's expectations and perceptions of their midwives' approach towards pain. Methods: The research took place in the largest public hospital in Jordan in the postnatal ward and involved midwives and their clients, including 60 midwives (out of 61 randomly sampled midwives), who responded to a study-specific validated survey for midwives (Survey Questionnaire for Midwives), and 360 women (out of 384 sampled women), who responded to a study-specific validated survey for women (Survey Questionnaire for Women). Findings: The study is ongoing. The findings are presented at the NJF conference (May 2016) and as a part of doctoral thesis that should be defended in May 2017.
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| 3. |
- Nilvér, Helena, et al.
(författare)
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Women's childbirth experiences in the Swedish Post-term Induction Study (SWEPIS) : a multicentre, randomised, controlled trial
- 2021
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Ingår i: BMJ Open. - : BMJ Publishing Group Ltd. - 2044-6055. ; 11:4
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To compare childbirth experiences in women randomly assigned to either induction of labour at 41 weeks or to expectant management until 42 weeks, in the Swedish Post-term Induction Study.DESIGN: A register-based, multicentre, randomised, controlled, superiority trial.SETTING: Women were recruited at 14 hospitals in Sweden, 2016-2018.PARTICIPANTS: Women with an uncomplicated singleton pregnancy were recruited at 41 gestational weeks.INTERVENTIONS: The women were randomly assigned to induction of labour at 41 weeks (induction group, n=1381) or expectant management until 42 weeks (expectant management group, n=1379).OUTCOME MEASURES: As main outcome, women's childbirth experiences were measured using the Childbirth Experience Questionnaire version 2 (CEQ2), in 656 women, 3 months after the birth at three hospitals. As exploratory outcome, overall childbirth experience was measured in 1457 women using a Visual Analogue Scale (VAS 1-10) within 3 days after delivery at the remaining eleven hospitals.RESULTS: The total response rate was 77% (2113/2760). There were no significant differences in childbirth experience measured with CEQ2 between the groups (induction group, n=354; expectant management group, n=302) in the subscales: own capacity (2.8 vs 2.7, p=0.09), perceived safety (3.3 vs 3.2, p=0.06) and professional support (3.6 vs 3.5, p=0.38) or in the total CEQ2 score (3.3 vs 3.2, p=0.07), respectively. Women in the induction group scored higher in the subscale participation (3.6 vs 3.4, p=0.02), although with a small effect size (0.19). No significant difference was observed in overall childbirth experience according to VAS (8.0 (n=735) vs 8.1 (n=735), p=0.22). CONCLUSIONS: There were no differences in childbirth experience, according to CEQ2 or overall childbirth experience assessed with VAS, between women randomly assigned to induction of labour at 41 weeks or expectant management until 42 weeks. Overall, women rated their childbirth experiences high.TRIAL REGISTRATION NUMBER: ISRCTN26113652.
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| 4. |
- Ahlberg, Erik, et al.
(författare)
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"Vi klimatforskare stödjer Greta och skolungdomarna"
- 2019
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Ingår i: Dagens nyheter (DN debatt). - 1101-2447.
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- DN DEBATT 15/3. Sedan industrialiseringens början har vi använt omkring fyra femtedelar av den mängd fossilt kol som får förbrännas för att vi ska klara Parisavtalet. Vi har bara en femtedel kvar och det är bråttom att kraftigt reducera utsläppen. Det har Greta Thunberg och de strejkande ungdomarna förstått. Därför stödjer vi deras krav, skriver 270 klimatforskare.
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| 5. |
- Andersson Svärd, Agnes, et al.
(författare)
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Decreased HLA-DQ expression on peripheral blood cells in children with varying number of beta cell autoantibodies
- 2020
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Ingår i: Journal of Translational Autoimmunity. - : Elsevier BV. - 2589-9090. ; 3
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Tidskriftsartikel (refereegranskat)abstract
- The risk for type 1 diabetes is strongly associated with HLA-DQ and the appearance of beta cell autoantibodies against either insulin, glutamate decarboxylase (GAD65), insulinoma-associated protein-2 (IA-2), or zinc transporter 8 (ZnT8). Prolonged exposure to autoantibodies may be related to T cell exhaustion known to occur in chronic infections or autoimmune disorders. It was hypothesized that autoantibody exposure may affect HLA-DQ expression on peripheral blood cells and thereby contribute to T cell exhaustion thought to be associated with the pathogenesis of type 1 diabetes. The aim of this study was to determine whether autoantibody exposure as an expression of autoimmunity burden was related to peripheral blood cell HLA-DQ cell surface expression in either 1) a cross-sectional analysis or 2) cumulative as area under the trajectory of autoantibodies during long term follow-up in the Diabetes Prediction in Skåne (DiPiS) study. Children (n = 67), aged 10-15 years were analyzed for complete blood count, HLA-DQ cell surface median fluorescence intensity (MFI), autoantibody frequency, and HLA genotypes by Next Generation Sequencing. Decreased HLA-DQ cell surface MFI with an increasing number of autoantibodies was observed in CD16+, CD14+CD16-, CD4+ and CD8+ cells but not in CD19+ cells and neutrophils. HLA-DQ cell surface MFI was associated with HLA-DQ2/8 in CD4+ T cells, marginally in CD14+CD16- monocytes and CD8+ T cells. These associations appeared to be related to autoimmunity burden. The results suggest that HLA-DQ cell surface expression was related to HLA and autoimmunity burden.
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| 6. |
- Andersson Svärd, Agnes, et al.
(författare)
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Possible Relationship between the HLA-DRA1 Intron Haplotype of Three Single-Nucleotide Polymorphisms in Intron 1 of the HLA-DRA1 Gene and Autoantibodies in Children at Increased Genetic Risk for Autoimmune Type 1 Diabetes
- 2022
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Ingår i: ImmunoHorizons. - : The American Association of Immunologists. - 2573-7732. ; 6:8, s. 614-629
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Tidskriftsartikel (refereegranskat)abstract
- Recently, a haplotype of three single-nucleotide polymorphisms (tri-SNP) in intron 1 of the HLA-DRA1 gene was found to be strongly associated with type 1 diabetes risk in HLA-DR3/3 individuals. The tri-SNP reportedly function as “expression quantitative trait loci,” modulating HLA-DR and -DQ expression. The aim was to investigate HLA-DRA1 tri-SNPs in relation to extended HLA class II haplotypes and human peripheral blood cell HLA-DQ cell-surface median fluorescence intensity (MFI), the first-appearing islet autoantibody, and autoimmunity burden. A total of 67 healthy subjects (10–15 y) at increased HLA risk for type 1 diabetes and with (n = 54) or without (n = 13) islet autoantibodies were followed longitudinally in the Diabetes Prediction in Skåne study. Among four tri-SNPs, AGG (n = 67), GCA (n = 47), ACG (n = 11), and ACA (n = 9), HLA-DQ cell-surface MFI on CD4+ T cells was lower in AGG than GCA (p = 0.030) subjects. Cumulative autoimmunity burden was associated with reduced HLA-DQ cell-surface MFI in AGG compared with GCA in CD16+ cells (p = 0.0013), CD4+ T cells (p = 0.0018), and CD8+ T cells (p = 0.016). The results suggest that HLA-DRA1 tri-SNPs may be related to HLA-DQ cell-surface expression and autoimmunity burden.
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| 7. |
- Blom, Hans
(författare)
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Purification Processes for Complex Biomacromolecules
- 2012
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis details various techniques and considerations for the purification of complex biomacromolecules. Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography. The enzyme was approximately 260-280 kDa in size with an apparent an unusual octagonal stoichiometry and displayed properties similar to other known plant α-mannosidases. Mucins were fractionated by ion exchange and size exclusion chromatography to assess the properties that govern the mucin surface coating interactions in biomaterial research. Commercially available mucins, of bovine and porcine origin, as wells as crude human mucin were tested. All showed to consist of a population of molecules which differ in size, charge and composition. The third part of the thesis concerns different aspects of plasmid DNA purification processes.A two-step method for analysis of plasmid DNA consisting of size exclusion followed by thiophilic adsorption chromatography was evaluated. It allowed determination of the supercoiled plasmid DNA concentration in all process steps without requirement for extensive sample preparation. This method was shown to be fully comparable in terms of accuracy to capillary gel electrophoresis, considered as the industry standard.Purification of plasmid DNA generally involves bacterial cell alkaline lysis, which creates a solution with flocculate material which needs to be removed prior to further processing. The addition of ammonium hydrogen carbonate to the suspension was evaluated to clarify the solution. The released carbon dioxide and ammonium lifts the flocculate to the surface and allows draining of a clear solution. The method is fully scalable, does not affect the plasmid DNA quality and requires no special equipment.Thiophilic adsorption chromatography was evaluated for simplification of an existing commercial large scale purification process and was shown to increase both product purity and yields of several tested plasmids. Also, implementation of this step significantly reduced overall production process time.
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| 8. |
- Brandhorst, Heide, 1962-, et al.
(författare)
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The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation
- 2009
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Ingår i: Transplantation. - 0041-1337 .- 1534-6080. ; 87:3, s. 370-5
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The isolation of islets from the human pancreas critically depends on an efficient enzyme blend. Previous studies have solely focused on the presence of collagenase and neutral protease/thermolysin. Despite improved characterization of these components, the lot-related variability in efficacy still persists suggesting that additional so far disregarded enzymes are required for efficient islet cleavage. METHODS: Varying activities of a tryptic-like enzyme were identified within collagenase NB1 lots, which were selected according to a matched ratio between tryptic-like and collagenase activity (TLA-ratio). Rat and human pancreata were processed with current standard procedures. RESULTS: Increasing the TLA-ratio from 1.3% to 10% reduced pancreas dissociation time in rats by 50% without affecting islet yield, viability, or posttransplant function in diabetic nude mice. Enhancing the TLA-ratio from 1.3% to 12.6% for human pancreas processing resulted in a significant reduction of recirculation time and increased incrementally human islet yield without affecting purity, in vitro function or recovery after culture. Optimized pancreas digestion correlated with a higher percentage of islet preparations fulfilling quality criteria for clinical transplantation. CONCLUSIONS: We conclude that TLA is an effective component that should be included in moderate amounts in enzyme blends for human islet isolation to optimize the efficiency and minimize the lot-related variability.
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| 9. |
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| 10. |
- Bäck, Helena, et al.
(författare)
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Svenska lärosätens ansvar vid mäns våld mot kvinnor och våld i nära relationer
- 2023
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Kvinnor riskerar att utsättas för våld oavsett var de lever och den farligaste platsen där de grövsta våldsbrotten sker är i kvinnors egna hem. För dem som utsätts för våld kan därför arbetsplatsen uppfattas som en trygg och stödjande plats. Dock tyder tidigare forskning på att våldet fortsätter på eller i närheten av arbetsplatsen, det är därför viktigt att det finns riktlinjer och rutiner som adresserar sådant våld. Syftet med denna rapport var att kartlägga om svenska lärosäten har riktlinjer och rutiner om mäns våld mot kvinnor och våld i nära relationer. I kartläggningen fann vi att enbart ett av 28 lärosäten har någon form av riktlinje/rutin som adresserar mäns våld mot kvinnor och våld i nära relationer. Dock saknar alla lärosäten riktlinjer och rutiner som vänder sig till studenter. När sådana saknas finns en risk att de som utsatts för våld inte uppmärksammats, vilket kan leda till individuella fysiska, psykiska och sociala konsekvenser, samt att arbetslivet påverkas negativt. I rapporten redovisar vi hur situationen ser ut vid Mittuniversitet, samt redogör för det förändringsarbete som vi initierat vid lärosätet. Vi hoppas att den här rapporten kan fungera som inspiration, inte bara gällande framtagande av riktlinjer och rutiner, utan också för framtida forskning, så att mäns våld kan upphöra och att de som utsätts för våld uppmärksammas och kan få adekvat hjälp och stöd.
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