| 1. |
- Melle, Giovanni, et al.
(författare)
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Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms
- 2020
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Ingår i: Frontiers in Bioengineering and Biotechnology. - : Frontiers Media S.A.. - 2296-4185. ; 8
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Tidskriftsartikel (refereegranskat)abstract
- High quality attenuated intracellular action potentials from large cell networks can be recorded on multi-electrode arrays by means of 3D vertical nanopillars using electrical pulses. However, most of the techniques require complex 3D nanostructures that prevent the straightforward translation into marketable products and the wide adoption in the scientific community. Moreover, 3D nanostructures are often delicate objects that cannot sustain several harsh use/cleaning cycles. On the contrary, laser optoacoustic poration allows the recording of action potentials on planar nanoporous electrodes made of noble metals. However, these constraints of the electrode material and morphology may also hinder the full exploitation of this methodology. Here, we show that optoacoustic poration is also very effective for porating cells on a large family of MEA electrode configurations, including robust electrodes made of nanoporous titanium nitride or disordered fractal-like gold nanostructures. This enables the recording of high quality cardiac action potentials in combination with optoacoustic poration, providing thus attenuated intracellular recordings on various already commercial devices used by a significant part of the research and industrial communities.
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| 2. |
- Verre, Ruggero, 1985, et al.
(författare)
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Polarization conversion-based molecular sensing using anisotropic plasmonic metasurfaces
- 2016
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Ingår i: Nanoscale. - : Royal Society of Chemistry. - 2040-3364 .- 2040-3372. ; 8:20, s. 10576-10581
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Tidskriftsartikel (refereegranskat)abstract
- Anisotropic media induce changes in the polarization state of transmitted and reflected light. Here we combine this effect with the refractive index sensitivity typical of plasmonic nanoparticles to experimentally demonstrate self-referenced single wavelength refractometric sensing based on polarization conversion. We fabricated anisotropic plasmonic metasurfaces composed of gold dimers and, as a proof of principle, measured the changes in the rotation of light polarization induced by biomolecular adsorption with a surface sensitivity of 0.2 ng cm(-2). We demonstrate the possibility of miniaturized sensing and we show that experimental results can be reproduced by analytical theory. Various ways to increase the sensitivity and applicability of the sensing scheme are discussed.
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| 3. |
- Ardini, Matteo, et al.
(författare)
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Live Intracellular Biorthogonal Imaging by Surface Enhanced Raman Spectroscopy using Alkyne-Silver Nanoparticles Clusters
- 2018
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Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 8:1
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Tidskriftsartikel (refereegranskat)abstract
- Live intracellular imaging is a valuable tool in modern diagnostics and pharmacology. Surface Enhanced Raman Spectroscopy (SERS) stands out as a non-destructive and multiplexed technique, but intracellular SERS imaging still suffers from interfering background from endogenous components. Here we show the assembly of small colloidal SERS probes with Raman signal in the cell-silent window of 1800–2900 cm−1 for biorthogonal intracellular SERS imaging of dopamine that was undistinguishable from the endogenous cell background. By linking colloidal silver nanoparticles with alkyne-dopamine adducts, clusters are formed by 2–6 nanoparticles spaced by tight interparticle gaps that exhibited high electric field enhancement and strong SERS signals of alkyne and dopamines. Due to the cell-silent signals of the alkyne, intracellular in-vitro Raman imaging shows that the dopamines on the internalized clusters remain distinguishable across the cytoplasm with good spatial resolution. Our method can be a general-purpose method for real-time imaging of biomolecules, such as proteins, peptides, DNA and drugs.
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| 4. |
- Banerjee, Shrobona, et al.
(författare)
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Porous aluminum decorated with rhodium nanoparticles : preparation and use as a platform for UV SERS
- 2024
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Ingår i: Materials Advances. - : Royal Society of Chemistry. - 2633-5409.
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Tidskriftsartikel (refereegranskat)abstract
- Currently, there is a high interest in novel plasmonic platforms and materials able to extend their applicability into the ultraviolet (UV) region of the electromagnetic spectrum. In the UV it is possible to explore the spectral properties of biomolecules that have only a small cross-section in the visible spectral range. However, most plasmonic metals have their resonances at wavelengths >350 nm. Aluminum and rhodium are two exceptions and therefore interesting candidate materials for UV plasmonics. In this work we developed a simple and low-cost preparation of functional substrates based on nanoporous aluminum decorated with rhodium nanoparticles. We demonstrate that these functionalized nanoporous metal films can be exploited as plasmonic materials for enhanced UV Raman spectroscopy.
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| 5. |
- Belotelov, V. I., et al.
(författare)
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Modern Magnetophotonic Materials and their Applications : introduction to special issue
- 2022
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Ingår i: Optical Materials Express. - : Optica Publishing Group. - 2159-3930 .- 2159-3930. ; 12:5, s. 2087-2089
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Magneto-optics was established almost two centuries ago by M. Faraday, who discovered the influence of magnetic field on light polarization [Diary, 1845]. We would like to present this special issue dedicated to the state-of-art in magnetophotonic materials and their applications.
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| 6. |
- Brown, Keith A., et al.
(författare)
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Machine Learning in Nanoscience : Big Data at Small Scales
- 2019
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Ingår i: Nano Letters. - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 20:1, s. 2-10
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Forskningsöversikt (refereegranskat)abstract
- Recent advances in machine learning (ML) offer new tools to extract new insights from large data sets and to acquire small data sets more effectively. Researchers in nanoscience are experimenting with these tools to tackle challenges in many fields. In addition to ML's advancement of nanoscience, nanoscience provides the foundation for neuromorphic computing hardware to expand the implementation of ML algorithms. In this Mini Review, we highlight some recent efforts to connect the ML and nanoscience communities by focusing on three types of interaction: (1) using ML to analyze and extract new insights from large nanoscience data sets, (2) applying ML to accelerate material discovery, including the use of active learning to guide experimental design, and (3) the nanoscience of memristive devices to realize hardware tailored for ML. We conclude with a discussion of challenges and opportunities for future interactions between nanoscience and ML researchers.
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| 7. |
- Caligiuri, Vincenzo, et al.
(författare)
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Dry synthesis of bi-layer nanoporous metal films as plasmonic metamaterial
- 2024
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Ingår i: Nanophotonics. - : Walter de Gruyter. - 2192-8606 .- 2192-8614. ; 13:7, s. 1159-1167
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Tidskriftsartikel (refereegranskat)abstract
- Nanoporous metals are a class of nanostructured materials finding extensive applications in multiple fields thanks to their unique properties attributed to their high surface area and interconnected nanoscale ligaments. They can be prepared following different strategies, but the deposition of an arbitrary pure porous metal is still challenging. Recently, a dry synthesis of nanoporous films based on the plasma treatment of metal thin layers deposited by physical vapour deposition has been demonstrated, as a general route to form pure nanoporous films from a large set of metals. An interesting aspect related to this approach is the possibility to apply the same methodology to deposit the porous films as a multilayer. In this way, it is possible to explore the properties of different porous metals in close contact. As demonstrated in this paper, interesting plasmonic properties emerge in a nanoporous Au–Ag bi-layer. The versatility of the method coupled with the possibility to include many different metals, provides an opportunity to tailor their optical resonances and to exploit the chemical and mechanical properties of components, which is of great interest to applications ranging from sensing, to photochemistry and photocatalysis.
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| 8. |
- Caligiuri, Vincenzo, et al.
(författare)
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Near- and Mid-Infrared Graphene-Based Photonic Architectures for Ultrafast and Low-Power Electro-Optical Switching and Ultra-High Resolution Imaging
- 2020
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Ingår i: ACS Applied Nano Materials. - : American Chemical Society (ACS). - 2574-0970. ; 3:12, s. 12218-12230
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Tidskriftsartikel (refereegranskat)abstract
- Confining near-infrared (NIR) and mid-infrared (MIR) radiation (1-10 mu m) at the nanoscale is one of the main challenges in photonics. Thanks to the transparency of silicon in the NIR-MIR range, optoelectronic systems like electro-optical modulators have been broadly designed in this range. However, the trade-off between energy-per-bit consumption and speed still constitutes a significant bottleneck, preventing such a technology to express its full potentialities. Moreover, the harmless nature of NIR radiation makes it ideal for bio-photonic applications. In this work, we theoretically showcase a new kind of electro-optical modulators in the NIR-MIR range that optimize the trade-off between power consumption, switching speed, and light confinement, leveraging on the interplay between graphene and metamaterials. We investigate several configurations among which the one consisting in a SiO2/graphene hyperbolic metamaterial (HMM) outstands. The peculiar multilayered configuration of the HMM allowed one also to minimize the equivalent electrical capacitance to achieve attoJoule electro/optical modulation at about 500 MHz switching speed. This system manifests the so-called dielectric singularity, in correspondence to which an HMM lens with resolving power of lambda/1660 has been designed, allowing to resolve 3 nm-wide objects placed at an interdistance of 3 nm and to overcome the diffraction limit by 3 orders of magnitude. The imaging possibilities opened by such technologies are evident especially in biophotonic applications, where the investigation of biological entities with tailored/broadband-wavelength radiation and nanometer precision is necessary. Moreover, the modulation performances demonstrated by the graphene-based HMM configure it as a promise for ultrafast and low-power opto-electronics applications.
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| 9. |
- Caprettini, Valeria, et al.
(författare)
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Enhanced Raman Investigation of Cell Membrane and Intracellular Compounds by 3D Plasmonic Nanoelectrode Arrays
- 2018
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Ingår i: Advanced Science. - : Wiley-VCH Verlagsgesellschaft. - 2198-3844. ; 5:12
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Tidskriftsartikel (refereegranskat)abstract
- 3D nanostructures are widely exploited in cell cultures for many purposes such as controlled drug delivery, transfection, intracellular sampling, and electrical recording. However, little is known about the interaction of the cells with these substrates, and even less about the effects of electroporation on the cellular membrane and the nuclear envelope. This work exploits 3D plasmonic nanoelectrodes to study, by surface-enhanced Raman scattering (SERS), the cell membrane dynamics on the nanostructured substrate before, during, and after electroporation. In vitro cultured cells tightly adhere on 3D plasmonic nanoelectrodes precisely in the plasmonic hot spots, making this kind of investigation possible. After electroporation, the cell membrane dynamics are studied by recording the Raman time traces of biomolecules in contact or next to the 3D plasmonic nanoelectrode. During this process, the 3D plasmonic nanoelectrodes are intracellularly coupled, thus enabling the monitoring of different molecular species, including lipids, proteins, and nucleic acids. Scanning electron microscopy cross-section analysis evidences the possibility of nuclear membrane poration compatible with the reported Raman spectra. These findings may open a new route toward controlled intracellular sampling and intranuclear delivery of genic materials. They also show the possibility of nuclear envelope disruption which may lead to negative side effects.
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| 10. |
- Carrara, Angelica, et al.
(författare)
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Plasmon Hybridization in Compressible Metal–Insulator–Metal Nanocavities : An Optical Approach for Sensing Deep Sub‐Wavelength Deformation
- 2020
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Ingår i: Advanced Optical Materials. - : Wiley-VCH Verlagsgesellschaft. - 2162-7568 .- 2195-1071. ; 8:18
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Tidskriftsartikel (refereegranskat)abstract
- A pressure-induced deformation-sensitive device (DSD) is presented based on 2D matrices of plasmonic gold nanodisks coupled to a metal thin layer through a compressible dielectric spacer, namely a deformable metal–insulator–metal (MIM) nanocavity, to report deep sub-wavelength size variations (<λ/200). The system is characterized by two hybrid branches, which are resonant in the visible/near infrared spectral region. The fundamental mode, owing to the near-field interaction between the plasmonic nanostructures and the metal film, exhibits a remarkable sensitivity to the gap size, exceeding that of a planar “macroscopic” optical cavity and extending its operational domain to the sub-wavelength range, where excellent opportunities toward truly multiscale MIMs-based pressure sensors can be envisioned. Concurrently, its intrinsic plasmonic nature synergistically combines into a single platform multi-purpose functionalities, such as ultrasensitive detection and remote temperature readout, with practical perspectives in ultra-compact inspection tools for structural and functional information at the nanoscale.
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