1. |
- Ekstrom, A., et al.
(författare)
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Optimized Chiral Nucleon-Nucleon Interaction at Next-to-Next-to-Leading Order
- 2013
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Ingår i: Physical Review Letters. - 1079-7114 .- 0031-9007. ; 110:19, s. Art. no. 192502-
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Tidskriftsartikel (refereegranskat)abstract
- We optimize the nucleon-nucleon interaction from chiral effective field theory at next-to-next-to-leading order (NNLO). The resulting new chiral force NNLOopt yields chi(2) approximate to 1 per degree of freedom for laboratory energies below approximately 125 MeV. In the A = 3, 4 nucleon systems, the contributions of three-nucleon forces are smaller than for previous parametrizations of chiral interactions. We use NNLOopt to study properties of key nuclei and neutron matter, and we demonstrate that many aspects of nuclear structure can be understood in terms of this nucleon-nucleon interaction, without explicitly invoking three-nucleon forces.
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2. |
- Ekstrom, A., et al.
(författare)
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Statistical uncertainties of a chiral interaction at next-to-next-to leading order
- 2015
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Ingår i: Journal of Physics G: Nuclear and Particle Physics. - : IOP Publishing. - 0954-3899 .- 1361-6471. ; 42:3
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Tidskriftsartikel (refereegranskat)abstract
- We have quantified the statistical uncertainties of the low-energy coupling-constants (LECs) of an optimized nucleon-nucleon interaction from chiral effective field theory at next-to-next-to-leading order. In addition, we have propagated the impact of the uncertainties of the LECs to two-nucleon scattering phase shifts, effective range parameters, and deuteron observables.
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3. |
- Wimberger, Sandra, 1987, et al.
(författare)
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Simultaneous inhibition of DNA-PK and Pol ϴ improves integration efficiency and precision of genome editing
- 2023
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Ingår i: Nature Communications. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical research and offers potential cures for genetic diseases. Despite rapid progress, low efficiency of targeted DNA integration and generation of unintended mutations represent major limitations for genome editing applications caused by the interplay with DNA double-strand break repair pathways. To address this, we conduct a large-scale compound library screen to identify targets for enhancing targeted genome insertions. Our study reveals DNA-dependent protein kinase (DNA-PK) as the most effective target to improve CRISPR/Cas9-mediated insertions, confirming previous findings. We extensively characterize AZD7648, a selective DNA-PK inhibitor, and find it to significantly enhance precise gene editing. We further improve integration efficiency and precision by inhibiting DNA polymerase theta (Pol ϴ). The combined treatment, named 2iHDR, boosts templated insertions to 80% efficiency with minimal unintended insertions and deletions. Notably, 2iHDR also reduces off-target effects of Cas9, greatly enhancing the fidelity and performance of CRISPR/Cas9 gene editing. Low efficiency of target DNA integration remains a challenge in genome engineering. Here the authors perform large-scale compound library and genetic screens to identify targets that enhance gene editing: they see that combined DNA-PK and Pol ϴ inhibition with potent compounds increases editing efficiency and precision.
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