| 1. |
- Cannadine, David, et al.
(författare)
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The country house : past, present, future
- 2018
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- From The Crown to Downton Abbey, the country house speaks to our fantasies of rustic splendor, style, and escape. Featuring three hundred photos from the National Trust, this lavish book draws back the curtain on the finest and most important historic homes in England, Wales, Scotland, and Ireland, revealing these great houses’ intriguing pasts, grand interiors, and vibrant reinventions for the enjoyment of modern-day visitors, residents, and armchair travelers. Locations include Knole, Cragside, Castle Howard, Chatsworth, Polesden Lacey, Petworth, Castle Bodiam, Blenheim, Longleat, and dozens more. Illuminating essays by country house expert Jeremy Musson, legendary British author and historian David Cannadine, and contributing writers and scholars provide unique insight into centuries of life in a historic home. This is a rich visual resource for lovers of sumptuous interiors on a human scale, as well as grand exterior architecture and gorgeous landscapes. For Anglophiles, royals watchers, and lovers of the country house lifestyle, architecture, and interior design, this is a magnificent new look at landmark British country houses, the treasures they contain, and how they speak to our fantasies of rustic splendor and escape today
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| 2. |
- Wacker, Soeren J., et al.
(författare)
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Identification of Selective Inhibitors of the Potassium Channel Kv1.1-1.2(3) by High-Throughput Virtual Screening and Automated Patch Clamp
- 2012
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Ingår i: ChemMedChem. - : Wiley. - 1860-7179 .- 1860-7187. ; 7:10, s. 1775-1783
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Tidskriftsartikel (refereegranskat)abstract
- Two voltage-dependent potassium channels, Kv1.1 (KCNA1) and Kv1.2 (KCNA2), are found to co-localize at the juxtaparanodal region of axons throughout the nervous system and are known to co-assemble in heteromultimeric channels, most likely in the form of the concatemer Kv1.11.2(3). Loss of the myelin sheath, as is observed in multiple sclerosis, uncovers the juxtaparanodal region of nodes of Ranvier in myelinated axons leading to potassium conductance, resulting in loss of nerve conduction. The selective blocking of these Kv channels is therefore a promising approach to restore nerve conduction and function. In the present study, we searched for novel inhibitors of Kv1.11.2(3) by combining a virtual screening protocol and electrophysiological measurements on a concatemer Kv1.11.2(3) stably expressed in Chinese hamster ovary K1 (CHO-K1) cells. The combined use of four popular virtual screening approaches (eHiTS, FlexX, Glide, and Autodock-Vina) led to the identification of several compounds as potential inhibitors of the Kv1.11.2(3) channel. From 89 electrophysiologically evaluated compounds, 14 novel compounds were found to inhibit the current carried by Kv1.11.2(3) channels by more than 80?% at 10 mu M. Accordingly, the IC50 values calculated from concentrationresponse curve titrations ranged from 0.6 to 6 mu M. Two of these compounds exhibited at least 30-fold higher potency in inhibition of Kv1.11.2(3) than they showed in inhibition of a set of cardiac ion channels (hERG, Nav1.5, and Cav1.2), resulting in a profile of selectivity and cardiac safety. The results presented herein provide a promising basis for the development of novel selective ion channel inhibitors, with a dramatically lower demand in terms of experimental time, effort, and cost than a sole high-throughput screening approach of large compound libraries.
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