| 1. |
- Manneberg, Otto, 1981-, et al.
(author)
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En struktur för ökad funktionell kunskap hos studenten från räkneövningar
- 2008
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Conference paper (peer-reviewed)abstract
- En struktur för räkneövningar med syftet att ge studenten ökade funktionella kunskaper och färdigheter i ämnet presenteras. Strukturen är avsedd att aktivera studenten både innan och under en räkneövning. Genom kamraträttning av frivilliga hemuppgifter frikopplade från bonussystem, student-student-diskussioner samt diskussion i helklass antas studenten uppmuntras till djupare lärstrategier och nå en högre taxonominivå inom ämnet.Strukturen utprovas på två studentgrupper; andraårsstudenter på ett civilingenjörsprogram på KTH, samt förstårsstudenter på optikerutbildningen på Karolinska Institutet som läser optikkurser vid KTH. Utvärdering sker under pågående kurs genom enkäter till alla studenterna samt intervjuer av optikerstudenter, samt efter fullgången kurs genom intervjuer av assistenter och ingenjörsstudenter. Resultaten visar att studenterna ställer sig positiva till det nya systemet och anser att det ger dem en djupare nivå av förståelse, att det skapar en mer transparent bild av rättningsprocessen, samt att det ökar interaktionen mellan studenter och skapar en bättre klassrumsmiljö. Deltagandet är stort bland optikerstudenterna, medan ingenjörsstudenterna anser att de har betydligt svårare att hitta tid till hemuppgifterna.
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| 3. |
- Grondin, Yohann, et al.
(author)
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Pulmonary delivery of d-methionine is associated with an increase in ALCAR and glutathione in cochlear fluids.
- 2013
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In: Hearing Research. - : Elsevier BV. - 0378-5955 .- 1878-5891. ; 298, s. 93-103
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Journal article (peer-reviewed)abstract
- In animals, hearing loss resulting from cochlear mechanosensory cell damage can be mitigated by antioxidants such as d-methionine (d-met) and acetyl-l-carnitine (ALCAR). The systemic routes of administration of these compounds, that must of necessity transit trough the cochlear fluids, may affect the antioxidant levels in the cochlea and the resulting oto-protective effect. In this study, we analyzed the pharmacokinetics of [C]d-met in the cochlea and four other tissues after intratracheal (IT), intranasal (IN), and oral by gavage (OG) administration and compared it to intravenous administration (IV). We then analyzed the effect of these four routes on the antioxidant content of the cochlear fluids after d-met or ALCAR administration, by liquid chromatography/mass spectrometry. Our results showed that the concentration of methionine and ALCAR in cochlear fluids significantly increased after their respective systemic administration. Interestingly, d-met administration also contributed to an increase of ALCAR. Our results also showed that the delivery routes differently affected the bioavailability of administered [C]d-met as well as the concentrations of methionine, ALCAR and the ratio of oxidized to reduced glutathione. Overall, pulmonary delivery via IT administration achieved high concentrations of methionine, ALCAR, and oxidative-related metabolites in cochlear fluids, in some cases surpassing IV administration, while IN route appeared to be the least efficacious. To our knowledge, this is the first report of the direct measurements of antioxidant levels in cochlear fluids after their systemic administration. This report also demonstrates the validity of the pulmonary administration of antioxidants and highlights the different contributions of d-met and ALCAR allowing to further investigate their impact on oxidative stress in the cochlear microenvironment.
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| 4. |
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| 5. |
- Guldevall, Karolin, 1981-, et al.
(author)
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Microchip screening platform for assessment of cytotoxic effector cells
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Other publication (other academic/artistic)abstract
- Here we report a screening platform for assessment of the cytotoxic potential of individual natural killer (NK) or T cells within larger populations. Human primary NK cells or human Epstein-Barr virus (EBV)- specific T cells were distributed across a silicon-glass microchip containing 32 400 individual microwells loaded with target cells. Through fluorescence screening and automated image analysis the numbers of effector and live or dead target cells in each well could be assessed at different time-points after initial mixing. Cytotoxicity was also studied by time-lapse live-cell imaging in microwells quantifying the killing potential of individual NK cells. Although most resting NK cells (≈75%) were non-cytotoxic to the leukemia cell line K562, some NK cells were able to kill several (≥3) target cells within the 12 hours long experiment. We demonstrate that this assay can be used to enumerate and characterize cytotoxic cells, something that could find clinical applications, e.g. in the selection of donors for stem cell transplantation or generation of highly specific and cytotoxic cells for adoptive immunotherapy.
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| 6. |
- Hultström, Jessica, et al.
(author)
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Proliferation and viability of COS-7 cells trapped by standing-wave ultrasound in a microfluidic chip
- 2006
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In: Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference. - : Japan Academic Association Inc. ; , s. 449-451
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Conference paper (peer-reviewed)abstract
- We study cell viability after ultrasonic-standing-wave trapping of low cell numbers in a microfluidic chip by recultivation of the trapped cells. The cell proliferation rate is estimated by counting of initial and final cell numbers and shows normal cell growth. The results demonstrate the potential of ultrasonic standing waves as a tool for gentle and long-term manipulation of low cell numbers in microfluidic systems.
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| 7. |
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| 8. |
- Hultström, Jessica, et al.
(author)
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Viability of ultrasonically trapped cells
- 2005
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In: Proc. 9<sup>th</sup> Annual European Conference on Micro & Nanoscale Technologies for the Biosciences.
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Conference paper (peer-reviewed)
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