| 1. |
- Ahmad, Sarosh, et al.
(författare)
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A Compact CPW-Fed Ultra-Wideband Multi-Input-Multi-Output (MIMO) Antenna for Wireless Communication Networks
- 2022
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Ingår i: IEEE Access. - : Institute of Electrical and Electronics Engineers (IEEE). - 2169-3536. ; 10, s. 25278-25289
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Tidskriftsartikel (refereegranskat)abstract
- In this article, a compact coplanar waveguide (CPW) technique based ultra-wideband multiple-input-multiple-output (MIMO) antenna is proposed. The design is characterized by a broad impedance bandwidth starting from 3 GHz to 11 GHz. The overall size of the MIMO design is 60 x 60 mm(2) (1.24 x 1.24 lambda(2)(g) @ 3 GHz) with a thickness of 1.6 mm. To make the design ultra-wideband, the proposed MIMO antenna design has four jug-shaped radiating elements. The design is printed on a FR-4 substrate (relative permittivity of epsilon(r) = 4.4 and loss tangent of tan delta = 0.025). The polarization diversity phenomenon is realized by placing four antenna elements orthogonally. This arrangement increases the isolation among the MIMO antenna elements. The simulated results of the ultra-wideband MIMO antenna are verified by measured results. The proposed MIMO antenna has a measured diversity gain greater than 9.98, envelope correlation coefficient (ECC) less than 0.02, and good MIMO performance where the isolation is more than -20dB between the elements. The group delay, channel capacity loss (CCL), and the total active reflection coefficient (TARC) multiplexing efficiency and mean effective gain results are also analyzed. The group delay is found to be less than 1.2ns, CCL values calculated to be less than 0.4 bits/sec/Hz, while the TARC is below -10dB for the whole operating spectrum. The proposed design is a perfect candidate for ultra-wideband wireless communication systems and portable devices.
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| 2. |
- Bongcam Rudloff, Erik, et al.
(författare)
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Complete genome sequence of a plant associated bacterium Bacillus amyloliquefaciens subsp. plantarum UCMB5033
- 2014
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Ingår i: Standards in Genomic Sciences. - : Springer Science and Business Media LLC. - 1944-3277. ; 9, s. 718-725
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Tidskriftsartikel (refereegranskat)abstract
- Bacillus amyloliquefaciens subsp. plantarum UCMB5033 is of special interest for its ability to promote host plant growth through production of stimulating compounds and suppression of soil borne pathogens by synthesizing antibacterial and antifungal metabolites or priming plant defense as induced systemic resistance. The genome of B. amyloliquefaciens UCMB5033 comprises a 4,071,167 bp long circular chromosome that consists of 3,912 protein-coding genes, 86 tRNA genes and 10 rRNA operons.
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| 3. |
- Johansson, Anna H, et al.
(författare)
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Studies of plant colonisation by closely related Bacillus amyloliquefaciens biocontrol agents using strain specific quantitative PCR assays
- 2014
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Ingår i: Antonie van Leeuwenhoek. - : Springer Science and Business Media LLC. - 0003-6072 .- 1572-9699. ; 106, s. 1247-1257
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Tidskriftsartikel (refereegranskat)abstract
- Certain strains of Bacillus amyloliquefaciens can colonize plants and improve growth and stress management. In order to study these effects, bacterial growth dynamics on plants and in the rhizosphere are of interest calling for specific analytical tools. For that purpose, quantitative real-time PCR (qPCR) assays were developed in order to differentiate among three closely related B. amyloliquefaciens subsp. plantarum strains (UCMB5033, UCMB5036, UCMB5113) and to determine their levels with high accuracy. Oligonucleotide primers were designed for strain unique gene sequences and used for SYBR green based qPCR analysis. Standard curves covered a wide linear range (10(6)) of DNA amounts with the lowest detection level at 50 fg. Post-reaction melting curve analysis showed only a single product. Accurate threshold cycles were obtained, even in the presence of high excess of related Bacillus strains and total bacterial DNA from soil. Analysis of Bacillus colonisation after seed treatment of two oilseed rape cultivars (Oase and Ritz) grown on agar support showed a time dependent effect but that the bacteria mostly were found on root tissues and little on green tissues. The colonisation on plants grown in soil varied among the Bacillus strains where Oase seemed to house more bacteria than Ritz. Applied as a mixture, all three Bacillus strains co-existed on the roots of plants grown in soil. The qPCR assay in combination with other techniques will be a powerful tool to study plant interactions of these B. amyloliquefaciens biocontrol agents to further understand the requirements for successful interactions and improvement of plant properties.
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| 4. |
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| 5. |
- Manzoor, Shahid
(författare)
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A Sustainable Marriage of Telcos and Transp in the Era of Big Data: Are We Ready?
- 2018
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Ingår i: Lecture Notes Of The Institute For Computer Sciences, Social Informatics And Telecommunications Engineering. - Cham : Springer International Publishing. - 1867-8211. ; 245, s. 210-219
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The emerging smart city paradigm e.g., intelligent transport, smart grid and participatory sensing etc. is to advance the quality, performance and experience of urban citizten services through greater connectivity. This paradigm needs to collect data from citizens, various devices and assets that could be monitored, processed and analysed for the city governers to make better decision and also more efficiently manage those assests and resources. While the telecommunication and Internet are progressively being over-burdened and congested by the growing data transmission demands. To keep expanding the telecommunications and Internet infrastructures to accomodate these intensive data demands is costly and also the associated energy consumptions and carbon emissions could at long last wind up genuinely hurting the environment. To face this issue in the coming era of big data, we envision it will be best to utilize the established urban transport and road infrastructure and existing daily massive vehicular trips, to complement traditional option for data transmission. After detailing the current state-of-the-art, we consider the main challenges that need to be faced. Moreover, we define the main pillars to integrate the telecommunications and transport infrastructures, and also a proposal for the future urban network architecture.
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| 6. |
- Manzoor, Shahid, et al.
(författare)
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Complete genome sequence of Methanoculleus bourgensis strain MAB1, the syntrophic partner of mesophilic acetate-oxidising bacteria (SAOB)
- 2016
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Ingår i: Standards in Genomic Sciences. - : Springer Science and Business Media LLC. - 1944-3277. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- Methanoculleus bourgensis strain MAB1 has been identified as the hydrogenotrophic partner of mesophilic acetate-oxidising bacteria, a syntrophic relationship operating close to the thermodynamic equilibrium and of considerable importance in ammonia-rich engineered biogas processes. Methanoculleus bourgensis strain MAB1 belongs to the order Methanomicrobiales, family Methanomicrobiaceae, within the phylum Euryarchaeota. The genome shows a total size of 2,859,299 bp encoding 3450 predicted protein-encoding genes, of which only 1472 (43 %) have been assigned tentative functions. The genome encodes further 44 tRNA genes and three rRNA genes (5S, 16S and 23S rRNA). This study presents assembling and annotation features as well as genomic traits related to ammonia tolerance and methanogenesis.
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| 7. |
- Manzoor, Shahid
(författare)
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Computational and comparative investigations of syntrophic acetate-oxidising bacteria (SAOB) : genome-guided analysis of metabolic capacities and energy conserving systems
- 2014
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Today's main energy sources are the fossil fuels petroleum, coal and natural gas, which are depleting rapidly and are major contributors to global warming. Methane is produced during anaerobic biodegradation of wastes and residues and can serve as an alternative energy source with reduced greenhouse gas emissions. In the anaerobic biodegradation process acetate is a major precursor and degradation can occur through two different pathways: aceticlastic methanogenesis and syntrophic acetate oxidation combined with hydrogenotrophic methanogenesis. Bioinformatics is critical for modern biological research, because different bioinformatics approaches, such as genome sequencing, de novo assembly sequencing and transcriptomics sequencing are providing a distinctly better understanding at the genomic level by predicting genes and pathways and by deciphering the relationships between genotype and phenotype. This thesis describes the genomic analysis of three syntrophic acetate-oxidising bacteria (SAOB), namely Tepidanaerobacter acetatoxydans, Clostridium ultunense and Syntrophaceticus schinkii. These isolates have the ability to perform syntrophic acetate oxidation in the presence of a partner methanogen, which ultimately produces methane in the final step of anaerobic digestion. The genomes were assembled using NGS data and the genomic behaviour was determined through genome annotation. Metabolic pathway analysis revealed the physiological attributes of the SAOB regarding substrate utilisation, intermediate metabolism, energy conservation and genes of the Wood- Ljungdahl pathway, which are known to be involved in acetate oxidation. The results showed that the three SAOB use contrasting strategies for syntrophic acetate oxidation (SAO): T. acetatoxydans possesses all genes involved in the W-L pathway except formate dehydrogenase and thus requires a syntrophic formate-utilising methanogenic partner; S. schinkii possesses the complete set of genes required for the W-L pathway to oxidise acetate in the presence of a hydrogen-utilising methanogenic partner; and C. ultunense uses different ways to oxidise acetate because it does not contain the complete set of W-L pathway genes. Moreover, the three SAOB differ from each other as regards organisation of the W-L pathway genes operon.
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| 8. |
- Manzoor, Shahid, et al.
(författare)
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Draft Genome Sequence of Clostridium ultunense Strain Esp, a Syntrophic Acetate-Oxidizing Bacterium
- 2013
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Ingår i: Genome Announcements. - 2169-8287. ; 1, s. 1-2
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Tidskriftsartikel (refereegranskat)abstract
- Clostridium ultunense strain Esp belongs to the functional group of syntrophic acetate-oxidizing bacteria (SAOB), which have been identified as key organisms for efficient biogas production from protein-rich materials. Genome analysis and comparative genomics might aid us to define physiological features that are essential for maintaining this particular syntrophic lifestyle.
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| 9. |
- Manzoor, Shahid, et al.
(författare)
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First genome sequence of a syntrophic acetate-oxidizing bacterium, Tepidanaerobacter acetatoxydans strain re1
- 2013
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Ingår i: Genome Announcements. - 2169-8287. ; 1, s. 2 p.-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Syntrophic acetate-oxidizing bacteria (SAOB) have been identified as key organisms for efficient biogas production from protein-rich materials. Tepidanaerobacter acetatoxydans is the first reported SAOB for which the genome has been sequenced. Genome analysis will aid us in understanding the mechanisms regulating syntrophy, particularly energy-conserving and electron transfer mechanisms.
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| 10. |
- Manzoor, Shahid, et al.
(författare)
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Genome-Guided Analysis and Whole Transcriptome Profiling of the Mesophilic Syntrophic Acetate Oxidising Bacterium Syntrophaceticus schinkii
- 2016
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- Syntrophaceticus schinkii is a mesophilic, anaerobic bacterium capable of oxidising acetate to CO2 and H-2 in intimate association with a methanogenic partner, a syntrophic relationship which operates close to the energetic limits of microbial life. Syntrophaceticus schinkii has been identified as a key organism in engineered methane-producing processes relying on syntrophic acetate oxidation as the main methane-producing pathway. However, due to strict cultivation requirements and difficulties in reconstituting the thermodynamically unfavourable acetate oxidation, the physiology of this functional group is poorly understood. Genome-guided and whole transcriptome analyses performed in the present study provide new insights into habitat adaptation, syntrophic acetate oxidation and energy conservation. The working draft genome of Syntrophaceticus schinkii indicates limited metabolic capacities, with lack of organic nutrient uptake systems, chemotactic machineries, carbon catabolite repression and incomplete biosynthesis pathways. Ech hydrogenase, [ FeFe] hydrogenases, [ NiFe] hydrogenases, F1F0-ATP synthase and membrane-bound and cytoplasmic formate dehydrogenases were found clearly expressed, whereas Rnf and a predicted oxidoreductase/heterodisulphide reductase complex, both found encoded in the genome, were not expressed under syntrophic growth condition. A transporter sharing similarities to the high-affinity acetate transporters of aceticlastic methanogens was also found expressed, suggesting that Syntrophaceticus schinkii can potentially compete with methanogens for acetate. Acetate oxidation seems to proceed via the Wood-Ljungdahl pathway as all genes involved in this pathway were highly expressed. This study shows that Syntrophaceticus schinkii is a highly specialised, habitat-adapted organism relying on syntrophic acetate oxidation rather than metabolic versatility. By expanding its complement of respiratory complexes, it might overcome limiting bioenergetic barriers, and drive efficient energy conservation from reactions operating close to the thermodynamic equilibrium, which might enable S. schinkii to occupy the same niche as the aceticlastic methanogens. The knowledge gained here will help specify process conditions supporting efficient and robust biogas production and will help identify mechanisms important for the syntrophic lifestyle.
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