| 1. |
- Carninci, P, et al.
(författare)
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The transcriptional landscape of the mammalian genome
- 2005
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Ingår i: Science (New York, N.Y.). - : American Association for the Advancement of Science (AAAS). - 1095-9203 .- 0036-8075. ; 309:5740, s. 1559-1563
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Tidskriftsartikel (refereegranskat)abstract
- This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5′ and 3′ boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development.
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| 2. |
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| 3. |
- Ramilowski, JA, et al.
(författare)
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Functional annotation of human long noncoding RNAs via molecular phenotyping
- 2020
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Ingår i: Genome research. - : Cold Spring Harbor Laboratory. - 1549-5469 .- 1088-9051. ; 30:7, s. 1060-1072
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Tidskriftsartikel (refereegranskat)abstract
- Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-to-date lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.
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| 4. |
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| 5. |
- Andonova, Stanislava, 1977, et al.
(författare)
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Enhanced Low Temperature NOx Reduction Performance Over Bimetallic Pt/Rh-BaO Lean NOx Trap Catalysts
- 2013
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Ingår i: Topics in Catalysis. - : Springer Science and Business Media LLC. - 1572-9028 .- 1022-5528. ; 56:1-8, s. 68-74
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Tidskriftsartikel (refereegranskat)abstract
- The overall NSR operation was tested over a bimetallic Pt/Rh-BaO lean NO (x) trap (LNT) catalyst in the range of 473-673 K with simulated diesel exhausts and compared to monometallic 1 wt% Pt/BaO/gamma-Al2O3 and 0.5 wt% Rh/BaO/gamma-Al2O3 samples. The results showed the beneficial effect of the simultaneous presence of 0.5 wt% Pt and 0.25 wt% Rh on the catalytic performance under lean-burn conditions at low temperatures. It was observed that both Pt/BaO/gamma-Al2O3 and Rh/BaO/gamma-Al2O3, which both were mildly aged, have limited NO (x) reduction capacity at 473 K. However, combining Pt and Rh in the NO (x) storage catalyst assisted the NO (x) reduction process to occur at lower temperatures (473 K). One possible reason could be that the combined Pt and Rh sample was more resistant to aging. In addition, the NO2-TPD data showed that the presence of Rh into the Pt/BaO/gamma-Al2O3 system has a considerable effect on the spill-over process of NO (x) , accelerating the release of NO (x) at lower temperatures. These results were in a good agreement with the observed higher rate of oxygen release of the bimetallic Pt/Rh catalyst, leaving a significant number of noble metal sites available for adsorption at lower temperatures than that of the monometallic Pt sample. The superior NSR performance of the bimetallic Pt/Rh/BaO/gamma-Al2O3 catalyst under lean-burn conditions suggested the existence of synergetic promotion effect between the Pt and Rh components, increasing the NO (x) reduction efficiency in comparison with that of the monometallic Pt and Rh-BaO LNT catalysts.
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| 6. |
- Andonova, Stanislava, 1977, et al.
(författare)
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Mechanistic investigations of the promoting role of Rh on the NSR performance of NOx storage BaO-based catalysts
- 2013
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Ingår i: Applied Catalysis B: Environmental. - : Elsevier BV. - 0926-3373 .- 1873-3883. ; 132, s. 266-281
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Tidskriftsartikel (refereegranskat)abstract
- To determine the promoting effect of Rh on the overall NOx storage and reduction (NSR) performance, the studies in the current work were directed toward investigating the storage and release ability over Rh NOx storage BaO-based catalysts compared to Pt. In terms of the metal surface dispersion and the ability of the noble metals to release oxygen at lower temperatures, the synthesized catalysts were characterized by means of dynamic CO chemisorption (RT) and N2O dissociation (RI - 773 K). The NOx storage capacity and the thermal stability of the NOx adsorbed species formed on the surface were analyzed via NOx storage tests and temperature programmed desorption (TPD) without and in the presence of CO2 and H2O. In addition, experiments with lean and rich cycling were conducted at 473,573 and 673 K. The results from the N2O dissociation experiments showed the superior ability of Rh/Al and Rh/Ba/Al catalysts compared to Pt toward O-2 release from the catalytic surface at lower temperatures. In this work, we show that the presence of Rh into the BaO/gamma-Al2O3 system has a considerable effect on the spill-over process of NOx to the precious metal, controlling the subsequent desorption of NOx to occur at lower temperatures in comparison with that of the Pt catalysts. It is suggested a mechanism of NOx desorption where the lower temperature of O-2 release from the surface of Rh catalysts could leave a significant number of noble metal sites accessible for adsorption. Thus this could facilitate the rate of spill-over of NOx from the storage site (the surface sites on gamma-Al2O3 and those on BaO) to the noble metal and their desorption at lower temperatures. The limited NOx storage ability of the Rh-based BaO/gamma-Al2O3 catalysts under lean-burn conditions was found to originate from both low NO oxidation activity and NOx reduction activity, while the main limiting factor for the low NSR performance of the Pt-based catalysts was the limited regeneration ability during rich period.
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| 7. |
- Andonova, Stanislava, 1977, et al.
(författare)
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Micro-calorimetric studies of NO2 adsorption on Pt/BaO-supported on gamma-Al2O3 NOx storage and reduction (NSR) catalysts-Impact of CO2
- 2017
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Ingår i: Molecular Catalysis. - : Elsevier BV. - 2468-8231. ; 436, s. 43-52
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Tidskriftsartikel (refereegranskat)abstract
- The adsorption of NO2 on Pt/BaO/gamma Al2O3 catalyst has been investigated by micro calorimetry at atmospheric pressure, NOx storage tests and temperature-programmed desorption (TPD). The heat of adsorption of NO2 (Delta H-ads(NO2)) was determined over a wide range of NOx coverages, as the catalyst was exposed to 500/900 ppm NO2 in the absence/presence of 5% CO2 in the range of 423-773 K. The temperature dependent changes of Delta H-ads(NO2) verified the presence of energetically different NOx storage sites with different binding strength. The Delta H-ads(NO2) was found to follow a linear correlation versus temperature, ranging for example from -134.5 to -178.8 kJ/mol for NOx storage over Pt/BaO/gamma Al2O3 at 423-673 K. Thus, at high temperature mostly strongly bound nitrates were formed, while at lower temperature more loosely bound species were also present. Interestingly, the heat of adsorption was higher when using higher NO2 concentration, indicating more bulk barium nitrate formation. This is consistent with the TPD data where a clear high temperature peak was visible after adsorption using 900 ppm NO2 at 423 and 473 K, which was not the case for 500 ppm NO2. Moreover, the micro-calorimetric data also provided evidence in support of the detrimental effect of CO2 on the NOx uptake process. The heat released during the NOx storage in 500 ppm NO2 + 5% CO2 was determined to be significantly reduced ca. -97.8 kJ mol(-1) at 423 K, but ca. -134.5 kJ mol(-1) without CO2. Furthermore, our results show that it is critical to measure heat of adsorption for surface compounds since they are significantly different compared to thermodynamic data for bulk materials.
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| 8. |
- Barone, A., et al.
(författare)
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Clinical and Histological changes after ridge preservation with two xenografts: preliminary results from a multicentre randomized controlled clinical trial
- 2017
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Ingår i: Journal of Clinical Periodontology. - : Wiley. - 0303-6979. ; 44:2, s. 204-214
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: To evaluate and compare clinical and histological changes after ridge preservation procedures with those of spontaneous healing. Materials and Methods: Ninety patients were enrolled in the present randomized controlled clinical trial and underwent single-tooth extraction in the premolar/molar areas. Thirty sites were grafted with collagenated cortico-cancellous (coll), 30 sites with cortical (cort) porcine bone and 30 sites underwent natural healing. Primary (vertical and horizontal bone changes after 3 months) and secondary outcomes (histomorphometric after 3 months) were evaluated at implant placement. Results: The vertical bone changes at the grafted sockets were significantly (Rho < 0.0001) lower (0.30 mm for cort group and 0.57 mm for coll group) when compared to non-grafted sockets (2.10 mm for nat group). Moreover, the width reduction of the coll (0.93 mm) and cort (1.33 mm) groups was significantly lower (Rho < 0.0001) than the non-grafted group (3.60 mm). The analysis of subgroups attested that when premolar and molar sites were compared, the buccal bone loss appeared to be dependent both on tooth position and grafting material employed. Conclusion: The ridge preservation procedures had significantly better outcomes when compared to natural healing. The biomaterials did not differ for maintenance of bone width; even though, the bone height seemed to be better preserved with the cortical porcine bone.
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