| 1. |
- Harach, T., et al.
(författare)
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Reduction of Abeta amyloid pathology in APPPS1 transgenic mice in the absence of gut microbiota
- 2017
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7
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Tidskriftsartikel (refereegranskat)abstract
- Alzheimer's disease is the most common form of dementia in the western world, however there is no cure available for this devastating neurodegenerative disorder. Despite clinical and experimental evidence implicating the intestinal microbiota in a number of brain disorders, its impact on Alzheimer's disease is not known. To this end we sequenced bacterial 16S rRNA from fecal samples of Aβ precursor protein (APP) transgenic mouse model and found a remarkable shift in the gut microbiota as compared to non-transgenic wild-type mice. Subsequently we generated germ-free APP transgenic mice and found a drastic reduction of cerebral Aβ amyloid pathology when compared to control mice with intestinal microbiota. Importantly, colonization of germ-free APP transgenic mice with microbiota from conventionally-raised APP transgenic mice increased cerebral Aβ pathology, while colonization with microbiota from wild-type mice was less effective in increasing cerebral Aβ levels. Our results indicate a microbial involvement in the development of Abeta amyloid pathology, and suggest that microbiota may contribute to the development of neurodegenerative diseases.
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| 2. |
- Kovalenko, T. M., et al.
(författare)
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EFFECT OF LOW AND HIGH FAT DIETS ON THE HIPPOCAMPAL STRUCTURE IN APOE -/- KNOCKOUT MICE
- 2019
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Ingår i: Fiziologichnyi Zhurnal. - : National Academy of Sciences of Ukraine (Co. LTD Ukrinformnauka). - 2522-9028 .- 2522-9036. ; 65:4, s. 31-40
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Tidskriftsartikel (refereegranskat)abstract
- The morphological and biochemical studies of the hippocampal CA1 area in ApoE-deficient mice under the conditionsof dietary intake (8 weeks) with low (LF) and high (HF) fat content were performed. Methods of immunohistochemistry, light and electron microscopy, immunoassay and morphometric as well as statistical analyses were used. It was revealed that the morpho-functional state of the nerve cells of the hippocampus ApoE-/- mice highly depends on the fat content of the diet. A higher degree of neuroinflammation was observed in the LF-group than in the control and HF-groups, which was manifested in reactive astro- and microgliosis. The number of undamaged pyramidal neurons in the CA1 area of the hippocampus ApoE-/- mice in the LF-group significantly decreased compared to the control group and the HF-group. In animals with LF- and HF-diet the density of synaptic terminals, the number of mitochondria in them, as well as the concentration of cellular adhesion molecules were decreased. It was suggested that synaptogenesis and synaptic plasticity in higher degree depended on the shortage of аpolipoprotein E than on the fat content in the diet.
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