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- Kubler-Kielb, Joanna, et al.
(författare)
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Oligosaccharide conjugates of Bordetella pertussis and bronchiseptica induce bactericidal antibodies, an addition to pertussis vaccine.
- 2011
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 108:10, s. 4087-92
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Tidskriftsartikel (refereegranskat)abstract
- Pertussis is a highly contagious respiratory disease that is especially dangerous for infants and children. Despite mass vaccination, reported pertussis cases have increased in the United States and other parts of the world, probably because of increased awareness, improved diagnostic means, and waning vaccine-induced immunity among adolescents and adults. Licensed vaccines do not kill the organism directly; the addition of a component inducing bactericidal antibodies would improve vaccine efficacy. We investigated Bordetella pertussis and Bordetella bronchiseptica LPS-derived core oligosaccharide (OS) protein conjugates for their immunogenicity in mice. B. pertussis and B. bronchiseptica core OS were bound to aminooxylated BSA via their terminal Kdo residues. The two conjugates induced similar anti-B. pertussis LPS IgG levels in mice. B. bronchiseptica was investigated because it is easier to grow than B. pertussis. Using B. bronchiseptica genetically modified strains deficient in the O-specific polysaccharide, we isolated fractions of core OS with one to five repeats of the terminal trisaccharide, having at the nonreducing end a GlcNAc or GalNAc, and bound them to BSA at different densities. The highest antibody levels in mice were elicited by conjugates containing an average of 8-17 OS chains per protein and with one repeat of the terminal trisaccharide. Conjugate-induced antisera were bactericidal against B. pertussis, and the titers correlated with ELISA-measured antibody levels (r = 0.74). Such conjugates are easy to prepare and standardize; added to a recombinant pertussis toxoid, they may induce antibacterial and antitoxin immunity.
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| 3. |
- Lewis, Matt, et al.
(författare)
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A standardised tidal-stream power curve, optimised for the global resource
- 2021
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Ingår i: Renewable Energy. - : Elsevier BV. - 0960-1481 .- 1879-0682. ; 170, s. 1308-1323
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Tidskriftsartikel (refereegranskat)abstract
- Tidal-stream energy resource can be predicted deterministically, provided tidal harmonics and turbine-device characteristics are known. Many turbine designs exist, all having different characteristics (e.g. rated speed), which creates uncertainty in resource assessment or renewable energy system-design decision-making. A standardised normalised tidal-stream power-density curve was parameterised with data from 14 operational horizontal-axis turbines (e.g. mean cut-in speed was ∼30% of rated speed). Applying FES2014 global tidal data (1/16° gridded resolution) up to 25 km from the coast, allowed optimal turbine rated speed assessment. Maximum yield was found for turbine rated speed ∼97% of maximum current speed (maxU) using the 4 largest tidal constituents (M2, S2, K1 and O1) and ∼87% maxU for a “high yield” scenario (highest Capacity Factor in top 5% of yield cases); with little spatial variability found for either. Optimisation for firm power (highest Capacity Factor with power gaps less than 2 h), which is important for problematic or expensive energy-storage cases (e.g. off-grid), turbine rated speed of ∼56% maxU was found – but with spatial variability due to tidal form and maximum current speed. We find optimisation and convergent design is possible, and our standardised power curve should help future research in resource and environmental impact assessment.
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