| 1. |
- Bannister, R., et al.
(författare)
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Afuni, a novel transforming growth factor-β gene is involved in arm regeneration by the brittle star Amphiura filiformis
- 2005
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Ingår i: Development Genes and Evolution. - : Springer Science and Business Media LLC. - 0949-944X .- 1432-041X. ; 215:8, s. 393-401
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Tidskriftsartikel (refereegranskat)abstract
- The bone morphogenetic proteins (BMPs) are a family of the transforming growth factor-beta (TGF-beta) superfamily that perform multiple roles during vertebrate and invertebrate development. Here, we report the molecular cloning of a novel BMP from regenerating arms of the ophiuroid Amphiura filiformis. The theoretically translated amino acid sequence of this novel BMP has high similarity to that of the sea urchin BMP univin. This novel BMP has been named afuni. Whole-mount in situ hybridisation implicates afuni in arm regeneration. Expression occurs in distinct proximal and distal regions of late regenerates (3- and 5-week postablation). These sites are at different stages of regeneration, suggesting multiple roles for this gene in adult arm development. Cellular expression of this gene occurs in migratory cells within the radial water canal (RWC) of regenerating and nonregenerating arms. These migrating coelomocytes suggest a key role for the coelomic RWC as a source of the cellular material for use in arm regeneration by A. filiformis
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| 2. |
- Bannister, R., et al.
(författare)
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Coelomic expression of a novel bone morphogenetic protein in regenerating arms of the brittle star Amphiura filiformis
- 2008
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Ingår i: Development Genes and Evolution. - 0949-944X. ; 218:1, s. 33-38
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Tidskriftsartikel (refereegranskat)abstract
- The bone morphogenetic proteins (BMPs) are a family of signalling molecules involved in numerous developmental processes including cell fate determination in embryonic ectoderm of vertebrate and invertebrate species. Recently, published evidence has indicated that BMPs are involved in echinoderm adult tissue regeneration. We have cloned a novel member of the BMP2/4 subfamily from the ophiuroid echinoderm Amphiura filiformis, which we have named afBMP2/4. Whole-mount in-situ hybridisation performed on non-regenerating brittle star arms revealed that expression of afBMP2/4 is localised to the radial water canal (RWC) and that this expression is upregulated at segmental intervals along the arm. This observed expression pattern suggests a putative active role for this echinoderm BMP transcript in somatic growth and maintenance of the brittle star arm. Expression of this factor has also been observed in regenerating arms 2 weeks post-ablation, implicating the RWC as a source of cells for ophiuroid arm regeneration.
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| 3. |
- Patruno, M., et al.
(författare)
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Anbmp2/4 is a new member of the transforming growth factor-beta superfamily isolated from a crinoid and involved in regeneration
- 2003
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Ingår i: Proceedings of the Royal Society B-Biological Sciences. - 0962-8452. ; 270:1522, s. 1341-1347
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Tidskriftsartikel (refereegranskat)abstract
- Invertebrates have frequently been used to help understand the complexities of regulatory gene function and evolution. The bone morphogenetic proteins (BMPs) are a highly conserved group of secreted regulatory factors that play an important part in early embryonic patterning. In the present study we have used the remarkable regenerative potential of crinoid echinoderms to explore the BMPs' site of expression in an adult developmental programme. Our results suggest that a crinoid BMP2/4 homologue is actively involved during the early stages of blastemal regeneration at a time when fundamental patterns are being established. This supports the idea of an evolutionary developmental programme where essential gene families are conserved throughout phylogeny in terms of both expression and function.
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