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- Gustavsson, Nadja, et al.
(författare)
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Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons
- 2021
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Ingår i: Light, science & applications. - : Springer Science and Business Media LLC. - 2047-7538. ; 10, s. 1-10
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Tidskriftsartikel (refereegranskat)abstract
- Alzheimer’s disease (AD) is the most common cause of dementia, costing about 1% of the global economy. Failures ofclinical trials targeting amyloid-βprotein (Aβ), a key trigger of AD, have been explained by drug inefficiency regardlessof the mechanisms of amyloid neurotoxicity, which are very difficult to address by available technologies. Here, wecombine two imaging modalities that stand at opposite ends of the electromagnetic spectrum, and therefore, can beused as complementary tools to assess structural and chemical information directly in a single neuron. Combininglabel-free super-resolution microspectroscopy for sub-cellular imaging based on novel optical photothermal infrared(O-PTIR) and synchrotron-based X-rayfluorescence (S-XRF) nano-imaging techniques, we capture elementaldistribution andfibrillary forms of amyloid-βproteins in the same neurons at an unprecedented resolution. Our resultsreveal that in primary AD-like neurons, iron clusters co-localize with elevated amyloidβ-sheet structures and oxidizedlipids. Overall, our O-PTIR/S-XRF results motivate using high-resolution multimodal microspectroscopic approaches tounderstand the role of molecular structures and trace elements within a single neuronal cell.
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| 2. |
- Visscher, Pieter T., et al.
(författare)
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Modern arsenotrophic microbial mats provide an analogue for life in the anoxic Archean
- 2020
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Ingår i: Communications Earth & Environment. - : Springer Science and Business Media LLC. - 2662-4435. ; 1:1
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Tidskriftsartikel (refereegranskat)abstract
- Arsenic-consuming microbes of the hypersaline Laguna La Brava in Chile may be an analogue for early life during the anoxic conditions of the Archean, according to geochemical and metagenomic analyses of the extant microbial mats The earliest evidence of life captured in lithified microbial mats (microbialites) predates the onset of oxygen production and yet, modern oxygenic mats are often studied as analogs based on their morphological similarity and their sedimentological and biogeochemical context. Despite their structural similarity to fossil microbialites, the presence of oxygen in most modern microbial mats disqualifies them as appropriate models for understanding early Earth conditions. Here we describe the geochemistry, element cycling and lithification potential of microbial mats that thrive under permanently anoxic conditions in arsenic laden, sulfidic waters feeding Laguna La Brava, a hypersaline lake in the Salar de Atacama of northern Chile. We propose that these anoxygenic, arsenosulfidic, phototrophic mats are a link to the Archean because of their distinctive metabolic adaptations to a reducing environment with extreme conditions of high UV, vast temperature fluctuations, and alkaline water inputs from combined meteoric and volcanic origin, reminiscent of early Earth.
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