| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Chambers, Joshua R., et al.
(författare)
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Correcting for Systematic Underestimation of Topographic Glacier Aerodynamic Roughness Values From Hintereisferner, Austria
- 2021
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Ingår i: Frontiers in Earth Science. - : Frontiers Media SA. - 2296-6463. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Spatially-distributed values of glacier aerodynamic roughness (z0) are vital for robust estimates of turbulent energy fluxes and ice and snow melt. Microtopographic data allow rapid estimates of z0 over discrete plot-scale areas, but are sensitive to data scale and resolution. Here, we use an extensive multi-scale dataset from Hintereisferner, Austria, to develop a correction factor to derive z0 values from coarse resolution (up to 30 m) topographic data that are more commonly available over larger areas. Resulting z0 estimates are within an order of magnitude of previously validated, plot-scale estimates and aerodynamic values. The method is developed and tested using plot-scale microtopography data generated by structure from motion photogrammetry combined with glacier-scale data acquired by a permanent in-situ terrestrial laser scanner. Finally, we demonstrate the application of the method to a regional-scale digital elevation model acquired by airborne laser scanning. Our workflow opens up the possibility of including spatio-temporal variations of z0 within glacier surface energy balance models without the need for extensive additional field data collection.
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| 3. |
- Hofmann, Felix Martin, et al.
(författare)
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Post-Last Glacial Maximum glacier fluctuations in the southern Écrins massif (westernmost Alps) : insights from 10Be cosmic ray exposure dating
- 2019
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Ingår i: Boreas. - : Wiley. - 0300-9483 .- 1502-3885. ; 48:4, s. 1019-1041
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Tidskriftsartikel (refereegranskat)abstract
- Only a few chronological constraints on Lateglacial and Early Holocene glacier variability in the westernmost Alps have hitherto been obtained. In this paper, moraines of two palaeoglaciers in the southern Écrins massif were mapped. The chronology of the stabilization of selected moraines was established through the use of 10Be cosmic ray exposure (CRE) dating. The equilibrium line altitude (ELA) during moraine deposition was reconstructed assuming an accumulation area ratio (AAR) of 0.67. Ten pre-Little Ice Age (LIA) ice-marginal positions of the Rougnoux palaeoglacier were identified and seven of these have been dated. The 10Be CRE age of a boulder on the lowermost sampled moraine indicates that the landform may have been first formed during a period of stable glaciers at around 16.2±1.7 ka (kiloyears before AD 2017) or that the sampled boulder experienced pre-exposure to secondary cosmic radiation. The moraine was re-occupied or, alternatively, shaped somewhat before 12.2±0.6 ka when the ELA was lowered by 230 m relative to the LIA ELA. At least six periods of stable ice margins occurred thereafter when the ELA was 220–160 m lower than during the LIA. The innermost dated moraine stabilized at or before 10.9±0.7 ka. Three 10Be CRE ages from a moraine of the Prelles palaeoglacier indicate a period of stationary ice margins at or before 10.9±0.6 ka when the ELA was lowered by 160 m with respect to the end of the LIA. The presented 10Be CRE ages are in good agreement with those of moraines that have been attributed to the Egesen stadial. Assuming unchanged precipitation, summer temperature in the southern Écrins massif at ~12 ka must have been at least 2 °C lower relative to the LIA.
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| 4. |
- Pirk, Norbert, et al.
(författare)
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Spatial variability of CO2 uptake in polygonal tundra : Assessing low-frequency disturbances in eddy covariance flux estimates
- 2017
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Ingår i: Biogeosciences. - : Copernicus GmbH. - 1726-4170 .- 1726-4189. ; 14:12, s. 3157-3169
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Tidskriftsartikel (refereegranskat)abstract
- The large spatial variability in Arctic tundra complicates the representative assessment of CO2 budgets. Accurate measurements of these heterogeneous landscapes are, however, essential to understanding their vulnerability to climate change. We surveyed a polygonal tundra lowland on Svalbard with an unmanned aerial vehicle (UAV) that mapped ice-wedge morphology to complement eddy covariance (EC) flux measurements of CO2. The analysis of spectral distributions showed that conventional EC methods do not accurately capture the turbulent CO2 exchange with a spatially heterogeneous surface that typically features small flux magnitudes. Nonlocal (low-frequency) flux contributions were especially pronounced during snowmelt and introduced a large bias of -46 gCm-2 to the annual CO22 budget in conventional methods (the minus sign indicates a higher uptake by the ecosystem). Our improved flux calculations with the ogive optimization method indicated that the site was a strong sink for CO2 in 2015 (82 gCm2). Due to differences in light-use efficiency, wetter areas with lowcentered polygons sequestered 47% more CO2 than drier areas with flat-centered polygons. While Svalbard has experienced a strong increase in mean annual air temperature of more than 2K in the last few decades, historical aerial photographs from the site indicated stable ice-wedge morphology over the last 7 decades. Apparently, warming has thus far not been sufficient to initiate strong ice-wedge degradation, possibly due to the absence of extreme heat episodes in the maritime climate on Svalbard. However, in Arctic regions where ice-wedge degradation has already initiated the associated drying of landscapes, our results suggest a weakening of the CO2 sink in polygonal tundra.
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| 5. |
- Shahbaz, Muhammad, et al.
(författare)
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Spatial heterogeneity of soil carbon exchanges and their drivers in a boreal forest
- 2022
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Ingår i: Science of the Total Environment. - : Elsevier BV. - 1879-1026 .- 0048-9697. ; 831
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Tidskriftsartikel (refereegranskat)abstract
- Boreal forests have a large impact on the global greenhouse gas balance and their soils constitute an important carbon (C) reservoir. Mature boreal forests are typically a net CO2 sink, but there are also examples of boreal forests that are persistent CO2 sources. The reasons remain often unknown, presumably due to a lack of understanding of how biotic and abiotic drivers interact to determine the microbial respiration of soil organic matter (SOM). This study aimed at identifying the main drivers of microbial SOM respiration and CO2 and CH4 soil chamber-fluxes within dry and wet sampling areas at the mature boreal forest of Norunda, Sweden, a persistent net CO2 source. The spatial heterogeneity of the drivers was assessed with a geostatistical approach combined with stepwise multiple regression. We found that heterotrophic soil respiration increased with SOM content and nitrogen (N) availability, while the SOM reactivity, i.e., SOM specific respiration, was determined by soil moisture and N availability. The latter suggests that microbial activity was N rather than C limited and that microbial N mining might be driving old-SOM decomposition, which was observed through a positive correlation between soil respiration and its δ13C values. SOM specific heterotrophic respiration was lower in wet than in dry areas, while no such dependencies were found for chamber-based soil CO2 fluxes, implying that oxygen depletion resulted in lower SOM reactivity. The chamber-based soil CH4 flux differed significantly between the wet and dry areas. In the wet area, we observed net CH4 emission that was positively related to soil moisture and NH4+-N content. Taken together, our findings suggest that N availability has a strong regulatory effect on soil CO2 and CH4 emissions at Norunda, and that microbial decomposition of old-SOM to release bioavailable N might be partly responsible for the net CO2 emission at the site.
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| 6. |
- Smith, Thomas, et al.
(författare)
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A scale-dependent model to represent changing aerodynamic roughness of ablating glacier ice based on repeat topographic surveys
- 2020
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Ingår i: Journal of Glaciology. - : Cambridge University Press (CUP). - 0022-1430 .- 1727-5652. ; 66:260, s. 950-964
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Forskningsöversikt (refereegranskat)abstract
- Turbulent fluxes make a substantial and growing contribution to the energy balance of ice surfaces globally, but are poorly constrained owing to challenges in estimating the aerodynamic roughness length (z0). Here, we used structure from motion (SfM) photogrammetry and terrestrial laser scanning (TLS) surveys to make plot-scale 2-D and 3-D microtopographic estimations of z0 and upscale these to map z0 across an ablating mountain glacier. At plot scales, we found spatial variability in z0 estimates of over two orders of magnitude with unpredictable z0 trajectories, even when classified into ice surface types. TLS-derived surface roughness exhibited strong relationships with plot-scale SfM z0 estimates. At the glacier scale, a consistent increase in z0 of ∼0.1 mm d-1 was observed. Space-for-time substitution based on time since surface ice was exposed by snow melt confirmed this gradual increase in z0 over 60 d. These measurements permit us to propose a scale-dependent temporal z0 evolution model where unpredictable variability at the plot scale gives way to more predictable changes of z0 at the glacier scale. This model provides a critical step towards deriving spatially and temporally distributed representations of z0 that are currently lacking in the parameterisation of distributed glacier surface energy balance models.
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