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- Fahlgren, Anna, 1972-, et al.
(författare)
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A capsular incision leads to a fast osteoarthritic response, but also elevated levels of activated osteogenic protein-1 in rabbit knee joint cartilage
- 2006
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Ingår i: Scandinavian Journal of Medicine and Science in Sports. - : Wiley. - 0905-7188 .- 1600-0838. ; 16:6, s. 456-462
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Tidskriftsartikel (refereegranskat)abstract
- We studied whether a small capsular incision alone, or combined with meniscectomy could induce early osteoarthritic changes in the rabbit knee. Thirty-one rabbits were operated on with a capsular incision in the left knee and meniscectomy in the right knee. Another 12 rabbits were used as controls. The rabbits were killed 3, 6 and 12 weeks after surgery. Osteoarthritic changes in the articular cartilage were evaluated by the modified Mankin score. The subchondral bone was evaluated by scintimetry (99mTc-HDP) and semiquantitative grading of histological changes. Osteogenic protein (OP-1) in its mature and pro-form was examined by immunohistochemistry. Both a capsular incision and meniscectomy induced articular cartilage fibrillation and increased bone metabolic activity during the initial weeks after surgery. Capsular incision led to lesser changes than meniscectomy. Mature OP-1 was elevated, and its pro-form reduced, in meniscectomized knees. A similar pattern was observed in knees with capsular incision. Already 3 weeks after surgery, the articular cartilage and subchondral bone showed typical signs of early osteoarthritis (OA), and a reparative response was suggested by increased intensity of OP-1 staining. As these signs were also found in knees with capsular incision only, it appears that trauma-related factors such as increased bleeding and inflammation are critical for the development of OA. Copyright © Blackwell Munksgaard 2006.
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- Fahlgren, Anna, 1972-, et al.
(författare)
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TGF-β1 as a prognostic factor in the process of early osteoarthrosis in the rabbit knee
- 2001
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Ingår i: Osteoarthritis and Cartilage. - : Elsevier BV. - 1063-4584 .- 1522-9653. ; 9:3, s. 195-202
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Tidskriftsartikel (refereegranskat)abstract
- Objective To assess changes in knee joint fluid concentrations of transforming growth factor-β1 (TGF-β1) and proteoglycan (PG) fragments during the early course of post-traumatic osteoarthrosis (OA) after meniscectomy in the rabbit knee, and to ascertain whether the concentrations of these substances shortly after operation could be used as prognostic markers for the OA process.Design In 15 rabbits with medial meniscectomy in one knee and a sham operation in the other knee, synovial lavage fluid samples were taken repeatedly, before operation, every third week post-operatively until 12 weeks, thereafter every sixth week, and at death. Five rabbits each were killed at 13, 25 and 40 weeks. Synovial lavage fluid samples from five non-operated rabbits served as controls. At death, two histological scores were formed that characterized the highest (MAX) and the overall (ALL) degree of OA changes in each joint.Results TGF-β1 and PG fragment concentrations in synovial lavage fluid correlated highly (R=0.81, P< 0.001). Both OA scores were higher in meniscectomized than controls (P< 0.05). The synovial lavage fluid concentration of TGF-β1 at 3 weeks, but no other time point, correlated to the histological scores (ALL, R=0.58; MAX, R=0.52;P< 0.001).Conclusion Higher concentrations of TGF-β1 in synovial lavage fluid early after surgery seemed indicative for the later development of more severe OA changes in contrast to lower concentrations. The association between TGF-β1 and the changes found later in the cartilage was underlined by the high correlations between this substance and PG fragment concentrations in synovial lavage fluid at all time points.
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- Messner, Karola, 1956-
(författare)
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Meniscectomy, meniscal repair, and prosthetic substitution : Clinical and experimental investigations
- 1992
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This study comprising 7 papers is concerned with different treatment of meniscal injuries. The aim was to analyse today's clinical methods and the possibility for prosthetic substitution in the future. Open peripheral meniscal repair was compared with partial arthroscopic meniscectomy in a 7·year follow-up evaluation of 130 patients. The reoperation rate was about 25% after both alternatives. The patients' willingness to undergo repair repair, however, was low because of the long rehabilitation period.Meniscal repair led to- greater improvement in function than did partial meniscectomy and was associated with a lesser degree of osteoarthritis. Knees with anterior cruciate ligament deficiency followed a clinical course-dominated by the instability.Meniscal substitution with a prosthesis ofpolyurethane·coated Dacron or Teflon or uncoated Teflon was studied in 114 rabbits. Despite their large size and mechanics different from those of a nonnal rabbit meniscus the Dacron prostheses became incorporated, with fibrous tissue ingrowth. Knees furnished with such a prosthesis developed less osteoartbritis on the tibial surface than did meniscectomized knees, but synovitis and osteophyte fonnation were common. Knees subjected to sham operation or meniscal inciSion showed no such changes. Knees furnished with a modified prosthesis of appropriate size and with improved material mechauics developed no naked·eye cartilage changes, but osteophyte fonnation, cartilage softening, and synovitis continued to appear. Despite better in·vitro mechanics, uncoated prostheses gave inferior results owing to changes in shape and to debris fonnation during the implantation period. In A CL-deficient knees the benefits of a prosthesis were seen only during the first 6 weeks, when the prosthesis had been implanted immediately after meniscectomy. After 3 months differences in treatment could no longer be distinguished owing to the overall effects of ACL resection.
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| 9. |
- Messner, Karola, 1956-, et al.
(författare)
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Radiographic joint space narrowing and histologic changes in a rabbit meniscectomy model of early knee osteoarthrosis
- 2001
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Ingår i: American Journal of Sports Medicine. - 0363-5465 .- 1552-3365. ; 29:2, s. 151-160
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study was to compare weightbearing radiographs with histologic cartilage evaluation in a rabbit meniscectomy model of the early stage of osteoarthrosis. Fifteen rabbits had a medial meniscectomy performed in one knee and a sham operation in the other knee. Five rabbits each were sacrificed at 13, 25, and 40 weeks after surgery. Radiographic joint space width and histologic cartilage changes of the medial knee compartment were quantified. Five nonoperated knees and five knees in which the meniscus had been removed immediately before the evaluations served as control specimens. Overall, the joint space of the peripheral part of the medial knee compartment was narrower in knees operated on for meniscus removal than in sham-operated knees (P < 0.003). In the knees with the meniscus removed, more cartilage changes were seen at the joint surface area of contact on radiographs than in the sham-operated knees (P < 0.0015). Indeed, the area of contact had cartilage changes similar to those in the whole medial compartment. However, there was no correlation between the degree of histologic cartilage change and the corresponding joint space measurements. Joint space width as measured on weightbearing radiographs is reduced after meniscectomy in the rabbit, but it does not reflect the degree of cartilage damage of the loaded joint surfaces in early stages of osteoarthrosis.
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| 10. |
- Messner, Karola, 1956-, et al.
(författare)
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Rat model of Achilles tendinitis
- 1999
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Ingår i: Acta Anatomica. - 0001-5180 .- 1421-9654. ; 165, s. 30-39
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