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| 2. |
- Andres, J, et al.
(författare)
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Transition-state structures for describing the enzyme-catalyzed mechanisms of rubisco
- 1999
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Ingår i: THEORETICAL CHEMISTRY ACCOUNTS. - : SPRINGER VERLAG. - 1432-881X. ; 101:1-3, s. 234-240
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The carboxylation and oxygenation processes of a model substrate, 3,4-dihydroxy-2-pentanone, have been theoreticaly characterized as a set of steps? mimicking the corresponding reactions of D-ribulose-1,5-bisphosphate catalyzed by rubisco. A theoretical c
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| 3. |
- Andres, J, et al.
(författare)
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Transition structures for hydride transfer reactions in vacuo and their role in enzyme catalysis
- 1996
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Ingår i: Journal of Molecular Structure: THEOCHEM. - : Elsevier. - 0166-1280. ; 371, s. 299-312
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Forskningsöversikt (refereegranskat)abstract
- A general discussion as to the role of in vacuo transition structure in enzyme catalysis is presented. Quantum mechanical aspects are emphasized. The transition structures defined as saddle points of index one (SPi-1) for the hydride transfer step on different model enzyme systems from flavoproteins to dehydrogenases have been characterized with analytical gradients at different levels of theory: semi-empirical; ab initio with different basis sets within the Hartree-Fock scheme; density functional theory using different approaches. Quantum chemical characteristics of the SPi-1 are used to discuss hydride transfer step in enzyme catalyzed reactions and mechanistic implications. With the exception of dihydrofolate reductase, the results for all other systems studied suggest that the endo relative orientation imposed by the active site on the reactants is essential for polarizing the CdHt bond and situating the system in the quadratic region of the endo SPi-1. The geometry and transition vector components are both model independent and weakly dependent on the level of theory used in their determination. Comparisons of the SPi-1 geometries with available X-ray coordinates show that the SPi-1 can be fitted without any stress at the active site. The geometrical arrangement of the SPi-1 results in optimal frontier LUMO orbital interactions, and the transition vector amplitudes show primary and secondary isotope effects to be strongly coupled. A comparison between simple and sophisticated molecular models shows that there is a minimal molecular model associated with geometrical parameters describing the essentials of the chemical interconversion step. For hydride transfer, the corresponding transition vector is an invariant feature.
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| 4. |
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| 5. |
- Denoyes-Rothan, B., et al.
(författare)
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Anthracnose on strawberry in France : Situation and perspectives
- 2006
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Ingår i: Proceedings of the Vth International Strawberry Symposium. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 277-280
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Konferensbidrag (refereegranskat)abstract
- Anthracnose, caused by Colletotrichum acutatum, on strawberry (Fragaria x ananassa) was first reported on fruit in France in 1981. Damage was later recorded on runners, petioles, foliage and crowns. The evolution of a disease control method, the molecular variability of C acutatum, the study of the interaction between pathogen and host, the different techniques of inoculation, and the last results concerning QTLs linked to resistance to C acutatum are reported, which cover 10 years of research in France.
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| 7. |
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| 8. |
- Hanke, L, et al.
(författare)
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A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo
- 2022
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Ingår i: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 13:1, s. 155-
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Tidskriftsartikel (refereegranskat)abstract
- Antibodies binding to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike have therapeutic promise, but emerging variants show the potential for virus escape. This emphasizes the need for therapeutic molecules with distinct and novel neutralization mechanisms. Here we describe the isolation of a nanobody that interacts simultaneously with two RBDs from different spike trimers of SARS-CoV-2, rapidly inducing the formation of spike trimer–dimers leading to the loss of their ability to attach to the host cell receptor, ACE2. We show that this nanobody potently neutralizes SARS-CoV-2, including the beta and delta variants, and cross-neutralizes SARS-CoV. Furthermore, we demonstrate the therapeutic potential of the nanobody against SARS-CoV-2 and the beta variant in a human ACE2 transgenic mouse model. This naturally elicited bispecific monomeric nanobody establishes an uncommon strategy for potent inactivation of viral antigens and represents a promising antiviral against emerging SARS-CoV-2 variants.
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| 9. |
- Moliner-Morro, A, et al.
(författare)
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Picomolar SARS-CoV-2 Neutralization Using Multi-Arm PEG Nanobody Constructs
- 2020
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Ingår i: Biomolecules. - : MDPI AG. - 2218-273X. ; 10:12
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Tidskriftsartikel (refereegranskat)abstract
- Multivalent antibody constructs have a broad range of clinical and biotechnological applications. Nanobodies are especially useful as components for multivalent constructs as they allow increased valency while maintaining a small molecule size. We here describe a novel, rapid method for the generation of bi- and multivalent nanobody constructs with oriented assembly by Cu-free strain promoted azide-alkyne click chemistry (SPAAC). We used sortase A for ligation of click chemistry functional groups site-specifically to the C-terminus of nanobodies before creating C-to-C-terminal nanobody fusions and 4-arm polyethylene glycol (PEG) tetrameric nanobody constructs. We demonstrated the viability of this approach by generating constructs with the SARS-CoV-2 neutralizing nanobody Ty1. We compared the ability of the different constructs to neutralize SARS-CoV-2 pseudotyped virus and infectious virus in neutralization assays. The generated dimers neutralized the virus similarly to a nanobody-Fc fusion variant, while a 4-arm PEG based tetrameric Ty1 construct dramatically enhanced neutralization of SARS-CoV-2, with an IC50 in the low picomolar range.
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| 10. |
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