| 1. |
- Lorén, Anders, 1974, et al.
(författare)
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Feasibility of quantitative determination of doxorubicin with surface-enhanced Raman spectroscopy
- 2001
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Ingår i: Journal of Raman Spectroscopy. - : Wiley. - 0377-0486 .- 1097-4555. ; 32:11, s. 971-974
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Tidskriftsartikel (refereegranskat)abstract
- Surface-enhanced Raman spectroscopy (SERS) was performed using excitation at 488 nm in a blood plasma-doxorubicin-silver colloid system. With a blood plasma content of 1%, a partial least-squares calibration of the doxorubicin was made in the 10-750 nM range. Predictions for a test set generated a root mean square error of prediction of 70 nM. The use of SERS and chemometrics in complex systems made it possible to use the highly informative Raman signals even at low concentrations without the need for sample pretreatment such as extraction. Copyright © 2001 John Wiley & Sons, Ltd.
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| 2. |
- Maurer, Matthew, et al.
(författare)
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3-Phosphoinositide-dependent kinase 1 potentiates upstream lesions on the phosphatidylinositol 3-kinase pathway in breast carcinoma
- 2009
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Ingår i: Cancer Research. - 1538-7445. ; 69:15, s. 306-6299
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Tidskriftsartikel (refereegranskat)abstract
- Lesions of ERBB2, PTEN, and PIK3CA activate the phosphatidylinositol 3-kinase (PI3K) pathway during cancer development by increasing levels of phosphatidylinositol-3,4,5-triphosphate (PIP(3)). 3-Phosphoinositide-dependent kinase 1 (PDK1) is the first node of the PI3K signal output and is required for activation of AKT. PIP(3) recruits PDK1 and AKT to the cell membrane through interactions with their pleckstrin homology domains, allowing PDK1 to activate AKT by phosphorylating it at residue threonine-308. We show that total PDK1 protein and mRNA were overexpressed in a majority of human breast cancers and that 21% of tumors had five or more copies of the gene encoding PDK1, PDPK1. We found that increased PDPK1 copy number was associated with upstream pathway lesions (ERBB2 amplification, PTEN loss, or PIK3CA mutation), as well as patient survival. Examination of an independent set of breast cancers and tumor cell lines derived from multiple forms of human cancers also found increased PDK1 protein levels associated with such upstream pathway lesions. In human mammary cells, PDK1 enhanced the ability of upstream lesions to signal to AKT, stimulate cell growth and migration, and rendered cells more resistant to PDK1 and PI3K inhibition. After orthotopic transplantation, PDK1 overexpression was not oncogenic but dramatically enhanced the ability of ERBB2 to form tumors. Our studies argue that PDK1 overexpression and increased PDPK1 copy number are common occurrences in cancer that potentiate the oncogenic effect of upstream lesions on the PI3K pathway. Therefore, we conclude that alteration of PDK1 is a critical component of oncogenic PI3K signaling in breast cancer.
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| 3. |
- Prikulis, J., et al.
(författare)
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Large-area Topography Analysis and Near-field Raman Spectroscopy using Bent Fibre Probes
- 2003
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Ingår i: Journal of Microscopy. - : Wiley. - 0022-2720 .- 1365-2818. ; 210:3, s. 269-273
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Tidskriftsartikel (refereegranskat)abstract
- We present a method for combined far-field Raman imaging, topography analysis and near-field spectroscopy. Surface-enhanced Raman spectra of Rhodamine 6G (R6G) deposited on silver nanoparticles were recorded using a bent fibre aperture-type near-field scanning optical microscope (NSOM) operated in illumination mode. Special measures were taken to enable optical normal-force detection for control of the tip–sample distance. Comparisons between far-field Raman images of R6G-covered Ag particle aggregates with topographic images recorded using atomic force microscopy (AFM) indicate saturation effects due to resonance excitation.
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| 4. |
- Eliasson, Charlotte, 1973, et al.
(författare)
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Multivariate evaluation of doxorubicin surface-enhanced Raman spectra.
- 2001
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Ingår i: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. - 1386-1425. ; 57:9, s. 1907-15
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Tidskriftsartikel (refereegranskat)abstract
- Multivariate evaluation of surface-enhanced Raman spectra of doxorubicin in plasma was performed. In a principal component analysis (PCA) all spectral features were modelled into three principal components. The major variation of the data was shown to be the variation of doxorubicin Raman signal together with the doxorubicin fluorescence, whereas the variation due to plasma was of minor importance. It was also shown that the surface-enhanced Raman scattering (SERS) measurements were independent on such factors as measurement occasion and silver colloids. The presented results show that with some improvements, quantification of doxorubicin directly in plasma could be possible.
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