| 1. |
- Enfors, Sven-Olof, et al.
(författare)
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Physiological responses to mixing in large scale bioreactors
- 2001
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Ingår i: Journal of Biotechnology. - 0168-1656 .- 1873-4863. ; 85:2, s. 175-185
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Tidskriftsartikel (refereegranskat)abstract
- Escherichia coli fed-batch cultivations at 22 m(3) scale were compared to corresponding laboratory scale processes and cultivations using a scale-down reactor furnished with a high-glucose concentration zone to mimic the conditions in a feed zone of the large bioreactor. Formate accumulated in the large reactor, indicating the existence of oxygen limitation zones. It is suggested that the reduced biomass yield at large scale partly is due to repeated production/reassimilation of acetate from overflow metabolism and mixed acid fermentation products due to local moving zones with oxygen limitation. The conditions that generated mixed-acid fermentation in the scale-down reactor also induced a number of stress responses, monitored by analysis of mRNA of selected stress induced genes. The stress responses were relaxed when the cells returned to the substrate limited and oxygen sufficient compartment of the reactor. Corresponding analysis in the large reactor showed that the concentration of mRNA of four stress induced genes was lowest at the sampling port most distant from the feed zone. It is assumed that repeated induction/relaxation of stress responses in a large bioreactor may contribute to altered physiological properties of the cells grown in large-scale bioreactor. Flow cytometric analysis revealed reduced damage with respect to cytoplasmic membrane potential and integrity in cells grown in the dynamic environments of the large scale reactor and the scale-down reactor.
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| 2. |
- Clason, Caroline C., et al.
(författare)
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Modelling the transfer of supraglacial meltwater to the bed of Leverett Glacier, Southwest Greenland
- 2015
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Ingår i: The Cryosphere. - : Copernicus GmbH. - 1994-0416 .- 1994-0424. ; 9:1, s. 123-138
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Tidskriftsartikel (refereegranskat)abstract
- Meltwater delivered to the bed of the Greenland Ice Sheet is a driver of variable ice-motion through changes in effective pressure and enhanced basal lubrication. Ice surface velocities have been shown to respond rapidly both to meltwater production at the surface and to drainage of supraglacial lakes, suggesting efficient transfer of meltwater from the supraglacial to subglacial hydrological systems. Although considerable effort is currently being directed towards improved modelling of the controlling surface and basal processes, modelling the temporal and spatial evolution of the transfer of melt to the bed has received less attention. Here we present the results of spatially distributed modelling for prediction of moulins and lake drainages on the Leverett Glacier in Southwest Greenland. The model is run for the 2009 and 2010 ablation seasons, and for future increased melt scenarios. The temporal pattern of modelled lake drainages are qualitatively comparable with those documented from analyses of repeat satellite imagery. The modelled timings and locations of delivery of meltwater to the bed also match well with observed temporal and spatial patterns of ice surface speed-ups. This is particularly true for the lower catchment (< 1000 m a.s.l.) where both the model and observations indicate that the development of moulins is the main mechanism for the transfer of surface meltwater to the bed. At higher elevations (e.g. 1250-1500 m a.s.l.) the development and drainage of supraglacial lakes becomes increasingly important. At these higher elevations, the delay between modelled melt generation and subsequent delivery of melt to the bed matches the observed delay between the peak air temperatures and subsequent velocity speed-ups, while the instantaneous transfer of melt to the bed in a control simulation does not. Although both moulins and lake drainages are predicted to increase in number for future warmer climate scenarios, the lake drainages play an increasingly important role in both expanding the area over which melt accesses the bed and in enabling a greater proportion of surface melt to reach the bed.
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| 3. |
- Kamenos, Nicholas A., et al.
(författare)
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Reconstructing Greenland ice sheet runoff using coralline algae
- 2012
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Ingår i: Geology. - 0091-7613 .- 1943-2682. ; 40:12, s. 1095-1098
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Tidskriftsartikel (refereegranskat)abstract
- The Greenland ice sheet (GrIS) contains the largest store of fresh water in the Northern Hemisphere, equivalent to similar to 7.4 m of eustatic sea-level rise, but its impacts on current, past, and future sea level, ocean circulation, and European climate are poorly understood. Previous estimates of GrIS melt, from 26 yr of satellite observations and temperature-driven melt models over 48 yr, show increasing melt trends. There are, however, no runoff data of comparable duration with which to validate the relationship between the spatial extent of melting and runoff or temperature-based runoff models. Further, longer runoff records are needed to extend the melt pattern of Greenland to centennial timescales, enabling recent observations and trends to be put into a better historical context. We have developed a new GrIS runoff proxy by extracting information on relative salinity changes from annual growth bands of red coralline algae. We observed significant negative relationships between historic runoff, relative salinity, and marine summer temperature in Sondre Stromfjord, Greenland. We produce the first reconstruction of runoff from a section of the GrIS that discharges into Sondre Stromfjord over several decades (1939-2002) and record a trend of increasing reconstructed runoff since the mid 1980s. In situ summer marine temperatures followed an equivalent trend. We suggest that since A. D. 1939, atmospheric temperatures have been important in forcing runoff. These results show that our technique has significant potential to enhance understanding of runoff from large ice sheets as it will enable melt reconstruction over centennial to millennial timescales.
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