| 1. |
- Nilsson, Jonas W., et al.
(författare)
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Solid-phase synthesis of libraries generated from a 4-phenyl-2-carboxy-piperazine Scaffold
- 2001
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Ingår i: Journal of Combinatorial Chemistry. - : American Chemical Society (ACS). - 2156-8952 .- 1520-4766 .- 1520-4774. ; 3:6, s. 546-553
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Tidskriftsartikel (refereegranskat)abstract
- Strategies for finding novel structures of therapeutical interest are discussed. The rationale for the selection of the two scaffolds N4-(m-aminophenyl)-piperazine-2-carboxylic acid E and N4-(o-aminophenyl)-piperazine-2-carboxylic F is described. The synthesis of the appropriate precursors to scaffold E and F and their use in solid-phase chemistry are described. A 160-member library was produced combining these novel piperazine scaffolds with eight sulfonyl chlorides/acid chlorides and 10 amines. The compound library prepared was analyzed using LC-MS, showing the expected base peak in all wells at an average purity of 82%.
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| 2. |
- Nilsson, Jonas W., et al.
(författare)
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Synthesis and SAR of Thrombin Inhibitors Incorporating a Novel 4-Amino-Morpholinone Scaffold : Analysis of X-ray Crystal Structure of Enzyme Inhibitor Complex
- 2001
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Ingår i: Journal of Medicinal Chemistry. - : American Chemical Society (ACS). - 0022-2623 .- 1520-4804. ; 46:19, s. 3985-4001
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Tidskriftsartikel (refereegranskat)abstract
- A 4-amino-2-carboxymethyl-3-morpholinone structural motif derived from malic acid has been used to mimic d-Phe-Pro in the thrombin inhibiting tripeptide d-Phe-Pro-Arg. The arginine in d-Phe-Pro-Arg was replaced by the more rigid P1 truncated p-amidinobenzylamine (Pab). These new thrombin inhibitors were used to probe the inhibitor binding site of α-thrombin. The best candidate in this series of thrombin inhibitors exhibits an in vitro IC50 of 0.130 μM. Interestingly, the stereochemistry of the 4-amino-2-carboxymethyl-3-morpholinone motif is reversed for the most active compounds compared to that of a previously reported 2-carboxymethyl-3-morpholinone series. The X-ray crystal structure of the lead inhibitor cocrystallized with α-thrombin is discussed.
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| 3. |
- Nilsson, Petra, 1980-, et al.
(författare)
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How to make a workplace health promotion questionnaire process applicable, meaningful, and sustainable
- 2011
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Ingår i: Journal of Nursing Management. - : Wiley-Blackwell. - 0966-0429 .- 1365-2834. ; 19:7, s. 906-914
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Tidskriftsartikel (refereegranskat)abstract
- Background In workplace health promotion, a questionnaire could be of great use. Unfortunately, fatigue regarding answering questionnaires has recently become greater than before. An action research approach could be a possible way of increasing employee participation.Aim This study reports an attempt to explore key aspects for participation in, and commitment to, a workplace health promotion questionnaire process.Method The study was conducted at two wards in a Swedish hospital. Data was collected during an action research process. Data were analysed with regard to a framework of questions.Findings The three key aspects for participation in, and commitment to, a workplace health promotion questionnaire process were: an applicable questionnaire, a meaningful questionnaire process and a continuous and sustainable questionnaire process. A structure is presented as practical advice to managers, describing how such a process could be established to be applicable, meaningful and sustainable.Conclusion This study has identified key aspects and prerequisites for questionnaire processes. The prerequisites – share decision-making, involve a core group and follow a structure – are discussed and proposed for managers and workgroups to consider in further workplace health promotion questionnaire processes.Implications for nursing management The key aspects and prerequisites presented could provide a stimulating standpoint or advice, useful for planning and accomplishing workplace questionnaire processes.
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| 4. |
- Nilsson, Petra, 1980-, et al.
(författare)
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The work experience measurement scale (WEMS) : a useful tool in workplace health promotion
- 2013
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Ingår i: Work. - : IOS Press. - 1051-9815 .- 1875-9270. ; 45:3, s. 379-387
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To present validity data for the Work Experience Measurement Scale (WEMS), an instrument measuring multifaceted work experience from a salutogenic health resource perspective as a contrast to the more common pathogenic risk perspective, by exploring WEMS relationship to established measurements that are positively related to health and work. A salutogenic perspective focuses on finding conditions and resources in life, for example at work, that can enhance the individual's health and strength, instead of those causing illness and weakness.METHOD: This study was carried out in 2009 at a Swedish hospital with a web-based survey (WEMS) to 770 employees. Different occupational groups at the hospital participated. Additional questionnaires used at the same time were the Utrecht Work Engagement Scale (UWES-9), the Salutogenic Health Indicator Scale (SHIS), the General Self-Efficacy scale (GSE), and three questions about self-rated health, general well-being, and quality of life.RESULTS: Cronbach's Alpha of WEMS sub-indices were in the interval of 0.85—0.96. Convergent validity and discriminant validity of WEMS and its sub-indices were shown to be satisfying by correlations. In addition, WEMS demonstrated the ability to discriminate between groups. WEMS sub-indices discriminated even better between groups than the total index.CONCLUSION: The WEMS proved to be a workplace health promotion questionnaire that was able to measure experiences of work from a salutogenic perspective. The WEMS has a potential of being a useful tool in workplace health promotion to enhance positive human capabilities and resources to improve work performance.
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| 5. |
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| 6. |
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| 7. |
- Dahlgren, Anders, et al.
(författare)
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Novel morpholinone-based D-Phe-Pro-Arg mimics as potential thrombin inhibitors : design, synthesis, and X-ray crystal structure of an enzyme inhibitor complex
- 2002
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Ingår i: Bioorganic & Medicinal Chemistry. - 0968-0896 .- 1464-3391. ; 10:6, s. 1829-1839
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Tidskriftsartikel (refereegranskat)abstract
- A morpholinone structural motif derived from d(+)- and l(−)-malic acid has been used as a mimic of d-Phe-Pro in the thrombin inhibiting tripeptide d-Phe-Pro-Arg. In place of Arg the more rigid P1 truncated p-amidinobenzylamine (Pab) or 2-amino-5-aminomethyl-3-methyl-pyridine have been utilized. The synthetic strategy developed readily delivers these novel thrombin inhibitors used to probe the α-thrombin inhibitor binding site. The best candidate in this series of thrombin inhibitors exhibits an in vitro IC50 of 720 nM. The X-ray crystal structure of this candidate co-crystallized with α-thrombin is discussed.
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| 8. |
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| 9. |
- Lindström, Petra Nilsson, et al.
(författare)
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Evaluating the usability of two salutogenic instruments on health and work experience, using cognitive interviewing
- 2018
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Ingår i: Journal of Workplace Behavioral Health. - : Routledge. - 1555-5240 .- 1555-5259. ; 33:3-4, s. 241-259
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Tidskriftsartikel (refereegranskat)abstract
- Workplace surveys are used in workplace health promotion as a basis for improvements at the workplace. But there is lack of psychometrically and qualitatively validated work-health related instruments with a salutogenic approach. The purpose of this study was, therefore, to evaluate the two instruments, the Salutogenic Health Indicator Scale and the Work Experience Measurement Scale, among staff of different professions in a healthcare setting. These instruments were evaluated with cognitive interviews conducted at a hospital in Sweden. The respondents were purposefully selected from various criteria such as profession, age, and sex (N = 14). The respondents read the items aloud and then spoke about how they experienced the items. A deductive (partly inductive) content analysis was done from Tourangeau's four concepts of respondent actions: comprehension, retrieval, judgment, and response. Two main categories emerged: (1) interpreting and (2) responding, and an additional six subcategories: difficulty, essence, direction, keywords, strategy, and alternatives. The results showed strengths and weaknesses of the instruments. The results were discussed from various validity aspects: face validity, content validity, and user validity. The validity aspects were connected to concepts of respondent actions as well as to questionnaire and respondent factors for motivation.
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| 10. |
- Nilsson, Charlotta, et al.
(författare)
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Element analysis of the eutardigrades Richtersius coronifer and Milnesium cf. asiaticum using Particle Induced X-ray Emission (PIXE)
- 2013
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Ingår i: Journal of limnology. - : Page Press. - 1129-5767 .- 1723-8633. ; 72:s1, s. 92-101
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Tidskriftsartikel (refereegranskat)abstract
- Semi-terrestrial tardigrades are well-known for their tolerance to a variety of environmental extremes, including desiccation, freezing and radiation. Despite several attempts to reveal the genetic and molecular mechanisms behind the resilience of tardigrades, it is still unknown how these animals are able to maintain the integrity of their cellular components under severe stress. Quantitative or qualitative changes in molecular compounds (e.g., carbohydrates, proteins) are expected, and have been the main line of research towards understanding the tolerance of tardigrades. In radiation tolerant bacteria, a tolerance mechanism based on manganese has been proposed. We evaluate this hypothesis in tardigrades and provide the first data on element composition in desiccated and non-desiccated specimens of two eutardigrade species, Richtersius coronifer and Milnesium cf. asiaticum. A focused 2 MeV proton microbeam was utilised to determine the elemental content, distributions and concentrations, using the ion beam analytical technique particle induced X-ray emission(PIXE). The presence of six elements – phosphorus, sulphur, chlorine, potassium, calcium and iron – were confirmed in all tardigrade specimens, at levels up to a few mg g–1. However, manganese was found in less than 10% of the analysed specimens, and in low amounts, thus our study provides no evidence for the manganese hypothesis. We also show that the distributions and/or concentrations of some elements differ between the two species as well as between the dehydrated and hydrated state. In particular, very low levels of iron were found in dehydrated M. cf. asiaticum. Our analysis shows that the PIXE technique is a useful tool for investigating questions on the distribution of elements both in dehydrated and hydrated tardigrades.
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