| 1. |
- Hernández-Neuta, Iván, 1986-, et al.
(författare)
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Highly multiplexed targeted sequencing strategy for infectious disease surveillance
- 2023
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Ingår i: BMC Biotechnology. - 1472-6750. ; 23
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Tidskriftsartikel (refereegranskat)abstract
- Background Global efforts to characterize diseases of poverty are hampered by lack of affordable and comprehensive detection platforms, resulting in suboptimal allocation of health care resources and inefficient disease control. Next generation sequencing (NGS) can provide accurate data and high throughput. However, shotgun and metagenome-based NGS approaches are limited by low concentrations of microbial DNA in clinical samples, requirements for tailored sample and library preparations plus extensive bioinformatics analysis. Here, we adapted molecular inversion probes (MIPs) as a cost-effective target enrichment approach to characterize microbial infections from blood samples using short-read sequencing. We designed a probe panel targeting 2 bacterial genera, 21 bacterial and 6 fungi species and 7 antimicrobial resistance markers (AMRs).Results Our approach proved to be highly specific to detect down to 1 in a 1000 pathogen DNA targets contained in host DNA. Additionally, we were able to accurately survey pathogens and AMRs in 20 out of 24 samples previously profiled with routine blood culture for sepsis.Conclusions Overall, our targeted assay identifies microbial pathogens and AMRs with high specificity at high throughput, without the need for extensive sample preparation or bioinformatics analysis, simplifying its application for characterization and surveillance of infectious diseases in medium- to low- resource settings.
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| 2. |
- Rydberg Sterner, Therese, et al.
(författare)
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The Gothenburg H70 Birth cohort study 2014-16: design, methods and study population.
- 2019
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Ingår i: European journal of epidemiology. - : Springer Science and Business Media LLC. - 1573-7284 .- 0393-2990. ; 34:2, s. 191-209
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Tidskriftsartikel (refereegranskat)abstract
- To improve health care for older persons, we need to learn more about ageing, e.g. identify protective factors and early markers for diseases. The Gothenburg H70 Birth Cohort Studies (the H70 studies) are multidisciplinary epidemiological studies examining representative birth cohorts of older populations in Gothenburg, Sweden. So far, six birth cohorts of 70-year-olds have been examined over time, and examinations have been virtually identical between studies. This paper describes the study procedures for the baseline examination of the Birth cohort 1944, conducted in 2014-16. In this study, all men and women born 1944 on specific dates, and registered as residents in Gothenburg, were eligible for participation (n=1839). A total of 1203 (response rate 72.2%; 559 men and 644 women; mean age 70.5years) agreed to participate in the study. The study comprised sampling of blood and cerebrospinal fluid, psychiatric, cognitive, and physical health examinations, examinations of genetics and family history, use of medications, social factors, functional ability and disability, physical fitness and activity, body composition, lung function, audiological and ophthalmological examinations, diet, brain imaging, as well as a close informant interview, and qualitative studies. As in previous examinations, data collection serves as a basis for future longitudinal follow-up examinations. The research gained from the H70 studies has clinical relevance in relation to prevention, early diagnosis, clinical course, experience of illness, understanding pathogenesis and prognosis. Results will increase our understanding of ageing and inform service development, which may lead to enhanced quality of care for older persons.
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| 3. |
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| 4. |
- Vicari, Marco, et al.
(författare)
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Spatial multimodal analysis of transcriptomes and metabolomes in tissues
- 2023
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Ingår i: Nature Biotechnology. - 1087-0156 .- 1546-1696.
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Tidskriftsartikel (refereegranskat)abstract
- We present a spatial omics approach that combines histology, mass spectrometry imaging and spatial transcriptomics to facilitate precise measurements of mRNA transcripts and low-molecular-weight metabolites across tissue regions. The workflow is compatible with commercially available Visium glass slides. We demonstrate the potential of our method using mouse and human brain samples in the context of dopamine and Parkinson’s disease.
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| 5. |
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| 6. |
- Borrie, Mats, et al.
(författare)
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The importance of stressful events during childhood on adult labour market outcome
- 2011
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- This paper examines if stressful events during childhood impact the labour market position in adulthood within the Swedish 1973 birth cohort. Empirical analyses are based on individual, longitudinal register data from the ASTRID database covering the total Swedish population between 1960-2008. We will analyze the different ways in which three specific events; parental separation, death of parent(s) and/or frequent migration, affect labour market outcome in an OLS-regression, controlling for family background characteristics. These events can occur in isola- tion or they can be interrelated. It is of importance to examine the effect of one single event as well as the accumulated effect of several events. The 1973 cohort makes an interesting case, since they both have experienced change in the nuclear family system during their childhood, and a period of recession and youth unemployment as well as cutbacks in social policy programmes during their age of labour market entrance. Our study thus focuses on how stressful events in childhood affect children’s life courses in turbulent times.
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| 7. |
- Bro, Tomas, et al.
(författare)
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Two point four million cataract surgeries: 30 years with the Swedish National Cataract Register, 1992-2021
- 2023
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Ingår i: Journal of cataract and refractive surgery. - : Wolters Kluwer. - 1873-4502 .- 0886-3350. ; 49:8, s. 879-884
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Tidskriftsartikel (refereegranskat)abstract
- The present review summarizes data collected by the Swedish National Cataract Register (NCR), which by the end of 2021 contained data for more than 2.4 million cataract surgeries between 1992 and 2021. During these 30 years, the cataract surgery rate rose from 3700 to 12800. The coverage of NCR is very high including 93% of all cataract procedures in Sweden between 2010 and 2021. Independently of demographic changes, the proportion of operations of patients age 60 to 79 has increased while the proportion of 80 to 90+ has decreased. The median visual acuity of the first eye planned for surgery was 0.1 decimal in 1992 and has increased to 0.5 decimal in 2021. Patient-reported outcome measures have been registered with the Catquest-9SF questionnaire since 2008, demonstrating intervention at an earlier stage, but consistently favorable outcomes. Surgical complications have decreased; endophthalmitis has decreased from 0.10% to below 0.02%, and posterior capsule rupture from 2.8% to 0.6%.
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| 8. |
- Cederkäll, Joakim, et al.
(författare)
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REX-ISOLDE - experiences from the first year of operation
- 2004
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Ingår i: Nuclear Physics A. - : Elsevier BV. - 0375-9474. ; 746, s. 17-17
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Konferensbidrag (refereegranskat)abstract
- The Radioactive beam EXperiment (REX) at CERN-ISOLDE has been commissioned and the first experiments successfully completed during 2002 and 2003. The concept used for post-acceleration has proven to be flexible and the experimental program has therefore been extended during the past year. This paper relays some experiences drawn from using the machine in the current program and also conveys some plans for the future.
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| 9. |
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| 10. |
- Edrén, Johannes, et al.
(författare)
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Modelica and Dymola for education in vehicle dynamics at KTH
- 2009
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Ingår i: Proceedings from 7th Modelica Conference 2009. - : Linköping University Electronic Press. ; , s. 775-783
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Konferensbidrag (refereegranskat)abstract
- Dymola and Modelica has been used at KTH Vehicle Dynamics (KTHVD) for research work since 2000, see e.g. [1]. With the Vehicle Dynamics Library (VDL) [2], Modelica has become far more accessible for both researchers and students in the field of vehicle dynamics. Therefore a project aiming at introducing it as a tool in education was initiated in order to evaluate the current state of Dymola and Modelica as tools for wider use in education at the division. The work presented in this paper was realized as a part of a PhD course, where one of the tasks were to design dedicated exercises to illustrate fundamentals of vehicle dynamics for students.
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