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- Ohlsson, Pelle, et al.
(författare)
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Microchip electroseparation of proteins using lipid-based nanoparticles
- 2010
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Ingår i: Electrophoresis. - : Wiley. - 0173-0835. ; 31:22, s. 3696-3702
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Tidskriftsartikel (refereegranskat)abstract
- Porous liquid crystalline lipid-based nanoparticles are shown here to enable protein analysis in microchip electroseparation by reducing sample adsorption. Additionally, higher stability and reproducibility of the separations were observed. The method was tested by separating green fluorescent protein (GFP) in hot embossed cyclic olefin polymer microchips with integrated fiber grooves for LIF detection. The sample adsorption was indirectly quantified by measuring the height, width and asymmetry of the separation peaks for various concentrations of nanoparticles in the sample and background electrolyte. Without nanoparticles, electropherograms displayed typical signs of extensive adsorption to the channel walls, with low, broad tailing peaks. Higher, narrower more symmetric peaks were generated when 0.5-10% nanoparticles were added, showing a dramatic reduction of sample adsorption. The current through the separation channel decreased with nanoparticle concentration, reducing to half its value when the nanoparticle concentration was increased from 0.5 to 4%. Addition of nanoparticles enabled separations that were otherwise hindered by extensive adsorption, e.g. separation of GFP mutants differing by only one amino acid. It was also observed that increasing the nanoparticle concentration increased the number of impurities that could be resolved in a GFP sample. This indicates that the adsorption is further reduced, and/or that the nanoparticles provide an interacting pseudostationary phase for electro-chromatography.
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| 2. |
- Ahlsten, Gunnar, 1948-, et al.
(författare)
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Dorsal Rhizotomy for Spasticity Management in Cerbral Palsy
- 2018
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Ingår i: Cerebral Palsy. - Cham : Springer International Publishing AG. ; , s. 1-10
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Bokkapitel (refereegranskat)abstract
- Selective dorsal rhizotomy (SDR) is a neurosurgical procedure for the relief of spasticity interfering with motor function in children with spastic cerebral palsy (CP). The goal of the treatment is to improve function as well as reduce pain and discomfort related to severely increased spasticity. SDR is an ablative procedure that results in lifelong effects on function in the central nervous system. One must also be aware that performing SDR does not guarantee that other treatments for spasticity or orthopedic corrective procedures can be avoided. For SDR to be an effective treatment, it must be combined with specific physiotherapy over a long period of time. Today there exists a good body of evidence that SDR is an effective means of treating patients with the CP subtype spastic diplegia, as long as selection criteria are rigorously adhered to. The procedure is also safe with little risk of short or long-term complications. Further studies on long-term effects late in adulthood will show if the treatment effects are stable over time.
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| 5. |
- Björgvinsson, Erling, et al.
(författare)
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Prototyping Futures
- 2012
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Prototyping Futures gives you a glimpse of what collaborating with academia might look like. Medea and its co-partners share their stories about activities happening at the research centre – projects, methods, tools, and approaches – what challenges lie ahead, and how these can be tackled. Examples of highlighted topics include: What is a living lab and how does it work? What are the visions behind the Connectivity Lab at Medea? And, how can prototyping-methods be used when sketching scenarios for sustainable futures? Other topics are: What is the role of the body when designing technology? What is collaborative media and how can this concept help us understand contemporary media practices? Prototyping Futures also discusses the open-hardware platform Arduino, and the concepts of open data and the Internet of Things, raising questions on how digital media and connected devices can contribute to more sustainable lifestyles, and a better world.
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| 10. |
- Decraene, Brecht, et al.
(författare)
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Decompressive craniectomy as a second/third-tier intervention in traumatic brain injury : A multicenter observational study
- 2023
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Ingår i: Injury. - : Elsevier. - 0020-1383 .- 1879-0267. ; 54:9
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: RESCUEicp studied decompressive craniectomy (DC) applied as third-tier option in severe traumatic brain injury (TBI) patients in a randomized controlled setting and demonstrated a decrease in mortality with similar rates of favorable outcome in the DC group compared to the medical management group. In many centers, DC is being used in combination with other second/third-tier therapies. The aim of the present study is to investigate outcomes from DC in a prospective non-RCT context.Methods: This is a prospective observational study of 2 patient cohorts: one from the University Hospitals Leuven (2008-2016) and one from the Brain-IT study, a European multicenter database (2003-2005). In thirty-seven patients with refractory elevated intracranial pressure who underwent DC as a second/third-tier intervention, patient, injury and management variables including physiological monitoring data and administration of thio-pental were analysed, as we l l as Extended Glasgow Outcome score (GOSE) at 6 months.Results: In the current cohorts, patients were older than in the surgical RESCUEicp cohort (mean 39.6 vs. 32.3; p < 0.001), had higher Glasgow Motor Score on admission (GMS < 3 in 24.3% vs. 53.0%; p = 0.003) and 37.8% received thiopental (vs. 9.4%; p < 0.001). Other variables were not significantly different. GOSE distribution was: death 24.3%; vegetative 2.7%; lower severe disability 10.8%; upper severe disability 13.5%; lower moderate disability 5.4%; upper moderate disability 2.7%, lower good recovery 35.1%; and upper good recover y 5.4%. The outcome was unfavorable in 51.4% and favorable in 48.6%, as opposed to 72.6% and 27.4% respectively in RESCUEicp (p = 0.02).Conclusion: Outcomes in DC patients from two prospective cohorts reflecting everyday practice were better than in RESCUEicp surgical patients. Mortality was similar, but fewer patients remained vegetative or severely disabled and more patients had a good recovery. Although patients were older and injury severity was lower, a potential partial explanation may be in the pragmatic use of DC in combination with other second/third-tier therapies in real-life cohorts. The findings underscore that DC maintains an important role in managing se-vere TBI.
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