| 1. |
- Andersson, Mats X., 1977, et al.
(författare)
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Involvement of the Electrophilic Isothiocyanate Sulforaphane in Arabidopsis Local Defense Responses
- 2015
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Ingår i: Plant Physiology. - : Oxford University Press (OUP). - 0032-0889 .- 1532-2548. ; 167:1
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Tidskriftsartikel (refereegranskat)abstract
- Plants defend themselves against microbial pathogens through a range of highly sophisticated and integrated molecular systems. Recognition of pathogen-secreted effector proteins often triggers the hypersensitive response (HR), a complex multicellular defense reaction where programmed cell death of cells surrounding the primary site of infection is a prominent feature. Even though the HR was described almost a century ago, cell-to-cell factors acting at the local level generating the full defense reaction have remained obscure. In this study, we sought to identify diffusible molecules produced during the HR that could induce cell death in naive tissue. We found that 4-methylsulfinylbutyl isothiocyanate (sulforaphane) is released by Arabidopsis (Arabidopsis thaliana) leaf tissue undergoing the HR and that this compound induces cell death as well as primes defense in naive tissue. Two different mutants impaired in the pathogen-induced accumulation of sulforaphane displayed attenuated programmed cell death upon bacterial and oomycete effector recognition as well as decreased resistance to several isolates of the plant pathogen Hyaloperonospora arabidopsidis. Treatment with sulforaphane provided protection against a virulent H. arabidopsidis isolate. Glucosinolate breakdown products are recognized as antifeeding compounds toward insects and recently also as intracellular signaling and bacteriostatic molecules in Arabidopsis. The data presented here indicate that these compounds also trigger local defense responses in Arabidopsis tissue.
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| 2. |
- Arnison, Tor, 1984-, et al.
(författare)
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Posttraumatic Stress among women with HIV in Zambia
- 2017
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Ingår i: The Medical Journal of Zambia. - Lusaka, Zambia : Zambia Medical Association. - 0047-651X. ; 44:2, s. 100-105
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To examine whether HIV-positive women in Lusaka District, Zambia, displays a higher degree of PTSD-symptoms than a HIV-negative control group.Method: The study targeted 50 HIV-positive women from four ART-clinics and 42 HIV-negative women from corresponding VCT-units. All sites were located in Lusaka District, Zambia. The HIV-positive women were compared with the control group in regard for PTSD, PTSD-symptoms, dissociative symptoms and history of traumatic experiences. The instruments used were PCL-C, DES-T and LYLES-A. Prior to the main study, the validity of the instruments were assessed with a pilot-sample.Results: Three participants in the HIV-positive group fulfilled the criteria for clinical PTSD (10.7 %), as compared to none in the control group. The HIV-positive group also displayed a significantly higher degree of PTSD-symptoms and previous traumatic experiences, with strong effect sizes, but not for dissociative symptoms. The significant difference in PTSD-symptoms remained while trauma-history was controlled for.Conclusions: The results of this study clearly indicates that women with HIV are vulnerable to PTSD and that contracting HIV in itself can constitute a psychological trauma in itself. Since PTSD among persons with HIV has been associated with transmission risk behaviours, reduced treatment adherence and a faster disease progression, these findings are important to consider in actions against HIV and AIDS.
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| 3. |
- Balling, Gitte, et al.
(författare)
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E-bogen. Skandinaviske perspektiver på forskning og uddannelse
- 2014
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Ingår i: Nordisk Tidsskrift for informations- og kulturformidling. - : Det Informationsvidenskablige Akademi, Köbenhavns Universitet. - 2245-2931 .- 2245-294X. ; 3:1, s. 5-19
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Tidskriftsartikel (refereegranskat)abstract
- Abstract: De skandinaviske lande har endnu ikke oplevet den samme udbredelse af e-bogen og påvirkning af bogmarkedet som det er tilfældet i de engelsksprogede lande. Alligevel er der en voksende interesse i e-bøger, ikke mindst i forsknings- og folkebibliotekerne. Denne artikel giver en oversigt over tre skandinaviske biblioteks- og informationsvidenskabelige skoler og deres forskning og undervisning i e-bøger. De respektive institutioner præsenterer deres aktuelle forskningsinteresser og -perspektiver på e-bøger og digital læsning, og hvorledes e-bøger er integreret i curriculum og uddannelser. Artiklen ender med at fremhæve fire områder, som artiklens forfattere mener, kalder på yderligere forskning inden for bibliotek- og informationsvidenskab:•Deep reading og læseoplevelser på digitale læseenheder • Den fysiske bogkulturs betydning for læsere • Aftalegrundlaget for udlån af e-bøger og ophavsret i biblioteker • E-bogens integration med andre teknologier
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| 4. |
- Berggren, Malin, 1975, et al.
(författare)
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Alternative EBNA1 expression in organ transplant patients.
- 2005
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Ingår i: Journal of medical virology. - : Wiley. - 0146-6615 .- 1096-9071. ; 76:3, s. 378-85
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Tidskriftsartikel (refereegranskat)abstract
- In order to identify patients at risk for developing post-transplant lymphoproliferative disease (PTLD), a sensitive nested RT-PCR method for detection of EBNA1 gene expression in peripheral blood cells was used. EBNA1 expression in peripheral blood samples from 60 organ recipients was analyzed and compared with 24 healthy controls in a retrospective study. Overall, EBNA1-positive samples were detected at least once in 43% of the transplant patients with post-transplant lymphoproliferative disease, in 18% of the other transplant patients and in none of the healthy controls. The odds ratio for EBNA1 expression in patients with post-transplant lymphoproliferative disease was 3.42 (95% CI=1.02-11.54) compared to other transplant recipients. Together with normal EBV Q promoter initiated EBNA1 transcripts, an alternatively spliced form was expressed in peripheral blood cells in the above-mentioned transplant patients. This transcript lacks the U leader exon in the 5'-untranslated region (UTR). We have previously identified and characterized a functional internal ribosome entry site, the EBNA IRES, in the untranslated U leader exon of EBNA1. Transfection experiments with EBNA1 coding plasmids followed by Western blot showed that the EBNA IRES promotes cap-independent translation and increases the EBNA1 protein level. The alternative EBNA1 transcript lacking this function is expressed in the majority of the investigated EBNA1-positive patient samples as well as in some EBV-positive B-cell lines. Alternative splicing in this form gives EBV potential to regulate the translation of EBNA1 by modifying the 5' UTR. These findings indicate a new mechanism for EBNA1 expression in vivo.
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| 5. |
- Birk Jørgensen, Morten, et al.
(författare)
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Les villes nordiques, une ingéniosité territoriale par nécessité
- 2019
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Ingår i: Perspective: actualité en histoire de l'art. - Paris : L'Institut national d'histoire de l'art. - 1777-7852 .- 2269-7721. ; :1, s. 69-96
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Tidskriftsartikel (refereegranskat)abstract
- Un débat entre Bilo Høegh Stigsen, Anja Kervanto Nevanlinna, Morten Birk Jørgensen, Tor Lindstrand et Håkan Nilsson, mené par Nicolas Escach
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| 6. |
- Bjursten, Eva-Lena, et al.
(författare)
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Computer programming in primary schools : Swedish Technology Teachers’ pedagogical strategies
- 2022
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Ingår i: International journal of technology and design education. - : Springer Nature. - 0957-7572 .- 1573-1804.
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Tidskriftsartikel (refereegranskat)abstract
- There is a recognized need for research on how to teach computer programming in primary schools in Sweden grades 4–6 (10–12-year-old pupils). Studies of teaching show the importance of teachers’ knowledge of content and pedagogy and how these two parts affect each other (i.e. pedagogical content knowledge [PCK]). Most teachers in Sweden have little or no formal education in computer programming, the revised Swedish curriculum requires them to teach it. The aim of this study is to explore the pedagogical strategies teachers use when they teach computer programming. Semi-structured interviews were conducted with 14 participants, comprising 12 teachers and 2 teacher trainers. The data were analysed deductively with themes from previous research. The results show that teachers use eight pedagogical strategies, including three new strategies that have been constructed inductively: do-it-yourself, gamification and progression. These eight pedagogical strategies are mostly general, and teachers may be considered regressed experts, as they lack formal training in computer programming. They facilitate learning in a general sense, but, compared to other subjects, their PCK in computer programming is problematized. In-service teacher training is needed to increase content knowledge, thus enabling to develop PCK in computer programming. It would also be fruitful to deepen our knowledge regarding pedagogical strategies in the PCK domain of computer programming.
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| 7. |
- Bjursten, Eva-Lena, et al.
(författare)
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Factors influencing Swedish grades 4–6 technology teachers’ choice of teaching and learning material in programming education
- 2023
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Ingår i: International journal of technology and design education. - : Springer Science and Business Media B.V.. - 0957-7572 .- 1573-1804.
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Tidskriftsartikel (refereegranskat)abstract
- There is a recognized need to understand the current state of programming implementation in the Swedish compulsory school system. This study focused specifically on the implementation of programming in the school subject of technology for grades 4–6. In Sweden, the responsibility for choosing teaching and learning material lies with individual teachers. Recent studies have indicated the prevalence of visual programming languages (VPLs) in classrooms. However, no empirical research has specifically investigated why teachers select particular programming learning environments (PLEs) and the challenges they have overcome in this process. Therefore, this study aimed to explore the PLEs used by teachers and the factors influencing their choices. In addition, this study explored the role of pedagogical content knowledge (PCK) and the influence of systemic and situational amplifiers and filters in shaping the programming education landscape, highlighting the importance of understanding these factors for effective implementation. Semi-structured interviews were conducted with 14 experienced programming teachers in grades 4–6 to gather insights. The results revealed that VPLs, particularly Scratch, have been widely adopted, but the study also identified three textual programming languages being utilized. Furthermore, the findings indicate that teachers’ previous education plays a significant role in shaping their PLE preferences. This suggests that programming education in both professional development and preservice teacher training is crucial for effective implementation. By investigating PLE choices and the factors influencing them, this study contributes to a better understanding of the current landscape of programming education in Sweden’s compulsory school system.
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| 8. |
- Broström, Tor, et al.
(författare)
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Climate Comfort Measurements in Swedish Churches Equipped with New Heating System
- 2008
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Ingår i: Proceedings of the 7th International Thermal Manikin and Modeling Meeting. - : University of Coimbra.
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Konferensbidrag (refereegranskat)abstract
- Many church buildings in Sweden have problems with rising heating costs combined with deterioration of inventories as a consequence of an inappropriate indoor climate. Gotland University has in collaboration with Gotland's churches installed a new radiant heating system for churches. The developed system has floor heating and bench heating pads combined with radiant heaters hanging from above the bench rows. These new installations have now been evaluated with respect to thermal climate comfort. The results show that the clothing of course plays an important role even with help radiant heaters and bench heat pads. Persons on both sides of the instrument as well as the height of the heaters do seem to play a smaller role in this investigation. An important parameter to check is the risk for drought that clearly is dependent on of the construction of the church benches.
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| 9. |
- Falk-Brynhildsen, Karin, 1959-
(författare)
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The effect of peroperative skin preparation on bacterial growth during cardiac surgery
- 2013
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Routine products are used and procedures are followed in order to prevent and minimize the bacterial contamination of the surgical wound, and thus reduce the risk of postoperative wound infections. The overall aim of this thesis was to investigate the effect of different preoperative skin preparation before cardiac surgery.In study I, 10 healthy volunteers were compared in time to recolonization of the skin and bacterial growth with or without plastic adhesive drape. Bacterial samples were taken as paired samples on both side of the sternum. Plastic drape on disinfected skin seems to hasten recolonization compared with bare skin.In study II, 135 cardiac surgery patients were comparing plastic adhesive drape versus bare skin on the chest regarding intra-operative bacterial growth. Plastic adhesive drape did not reduce the bacterial recolonization or wound contamination, P. acnes colonizes males more often than females and P. acnes is not affected by disinfection with 0.5% chlorhexidine in ethanol.Study III, compared the leg harvesting site with or without microbal skin sealant in 135 CABG patients regarding intraoperative bacterial growth and postoperative wound infection. Almost no bacterial growth was found during surgery regardless of the use of microbial skin sealant and bare skin. A high incidence of postoperative wound infections (16.8%) in 2 month follow up was present and SSI was largely caused by S. aureus, i.e. other bacterial species than observed intraoperative.Study IV, a descriptive study using phenotypic and genotypic methods investigate susceptibility to chlorhexidine among S. epidermidis indicating that S. epidermidis isolates following preoperative skin disinfection are sensitive tochlorhexidine.
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| 10. |
- Ferreira, David, et al.
(författare)
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Atlantic-Pacific Asymmetry in Deep Water Formation
- 2018
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Ingår i: Annual Review of Earth and Planetary Science. - : Annual Reviews. - 0084-6597 .- 1545-4495. ; 46, s. 327-352
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Forskningsöversikt (refereegranskat)abstract
- While the Atlantic Ocean is ventilated by high-latitude deep water formation and exhibits a pole-to-pole overturning circulation, the Pacific Ocean does not. This asymmetric global overturning pattern has persisted for the past 2-3 million years, with evidence for different ventilation modes in the deeper past. In the current climate, the Atlantic-Pacific asymmetry occurs because the Atlantic is more saline, enabling deep convection. To what extent the salinity contrast between the two basins is dominated by atmospheric processes (larger net evaporation over the Atlantic) or oceanic processes (salinity transport into the Atlantic) remains an outstanding question. Numerical simulations have provided support for both mechanisms; observations of the present climate support a strong role for atmospheric processes as well as some modulation by oceanic processes. A major avenue for future work is the quantification of the various processes at play to identify which mechanisms are primary in different climate states.
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