| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Porth, Oliver, et al.
(författare)
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The Event Horizon General Relativistic Magnetohydrodynamic Code Comparison Project
- 2019
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Ingår i: Astrophysical Journal, Supplement Series. - : American Astronomical Society. - 1538-4365 .- 0067-0049. ; 243:2
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Tidskriftsartikel (refereegranskat)abstract
- Recent developments in compact object astrophysics, especially the discovery of merging neutron stars by LIGO, the imaging of the black hole in M87 by the Event Horizon Telescope, and high- precision astrometry of the Galactic Center at close to the event horizon scale by the GRAVITY experiment motivate the development of numerical source models that solve the equations of general relativistic magnetohydrodynamics (GRMHD). Here we compare GRMHD solutions for the evolution of a magnetized accretion flow where turbulence is promoted by the magnetorotational instability from a set of nine GRMHD codes: Athena++, BHAC, Cosmos++, ECHO, H-AMR, iharm3D, HARM-Noble, IllinoisGRMHD, and KORAL. Agreement among the codes improves as resolution increases, as measured by a consistently applied, specially developed set of code performance metrics. We conclude that the community of GRMHD codes is mature, capable, and consistent on these test problems.
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| 3. |
- Ashworth, Eleanor K., et al.
(författare)
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Protomers of the green and cyan fluorescent protein chromophores investigated using action spectroscopy
- 2023
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Ingår i: Physical Chemistry, Chemical Physics - PCCP. - 1463-9076 .- 1463-9084. ; 25:30, s. 20405-20413
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Tidskriftsartikel (refereegranskat)abstract
- The photophysics of biochromophore ions often depends on the isomeric or protomeric distribution, yet this distribution, and the individual isomer contributions to an action spectrum, can be difficult to quantify. Here, we use two separate photodissociation action spectroscopy instruments to record electronic spectra for protonated forms of the green (pHBDI(+)) and cyan (Cyan(+)) fluorescent protein chromophores. One instrument allows for cryogenic (T = 40 & PLUSMN; 10 K) cooling of the ions, while the other offers the ability to perform protomer-selective photodissociation spectroscopy. We show that both chromophores are generated as two protomers when using electrospray ionisation, and that the protomers have partially overlapping absorption profiles associated with the S-1 & LARR; S-0 transition. The action spectra for both species span the 340-460 nm range, although the spectral onset for the pHBDI(+) protomer with the proton residing on the carbonyl oxygen is red-shifted by & AP;40 nm relative to the lower-energy imine protomer. Similarly, the imine and carbonyl protomers are the lowest energy forms of Cyan(+), with the main band for the carbonyl protomer red-shifted by & AP;60 nm relative to the lower-energy imine protomer. The present strategy for investigating protomers can be applied to a wide range of other biochromophore ions.
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| 4. |
- Cheung, Herman H., et al.
(författare)
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Monitor – Biology
- 2004
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Ingår i: Drug Discovery Today. - 1878-5832. ; 9:14, s. 621-622
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Tidskriftsartikel (refereegranskat)abstract
- The hottest developments in the fields of cancer research, neuroscience, genomics and proteomics, anti-virals and more, with a pick of the key research papers in these areas.
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| 5. |
- Cheung, Herman H., et al.
(författare)
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Monitor – Biology
- 2004
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Ingår i: Drug Discovery Today. - 1878-5832. ; 9:6, s. 287-290
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Tidskriftsartikel (refereegranskat)abstract
- The hottest developments in the fields of cancer research, neuroscience, genomics and proteomics, anti-virals and more, with a pick of the key research papers in these areas.
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| 6. |
- Collin, Mattias, et al.
(författare)
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Biology
- 2004
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Ingår i: Drug Discovery Today. - 1878-5832. ; 9:9, s. 420-422
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Tidskriftsartikel (refereegranskat)abstract
- The hottest developments in the fields of cancer research, neuroscience, genomics and proteomics, anti-virals and more, with a pick of the key research papers in these areas.
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| 7. |
- Collin, Mattias, et al.
(författare)
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Monitor – biology
- 2005
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Ingår i: Drug Discovery Today. - 1878-5832. ; 10:1, s. 75-77
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Tidskriftsartikel (refereegranskat)
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| 8. |
- Collin, Mattias, et al.
(författare)
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Monitor – Biology
- 2004
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Ingår i: Drug Discovery Today. - 1878-5832. ; 9:12, s. 539-541
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Tidskriftsartikel (refereegranskat)abstract
- An insight into the latest developments in drug discovery through brief synopses of recent publications, summarizing the chemistry, pharmacological significance and biological relevance of new molecules.
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| 9. |
- Heath, Victoria, et al.
(författare)
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Monitor – biology
- 2004
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Ingår i: Drug Discovery Today. - 1878-5832. ; 9:15, s. 678-681
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Tidskriftsartikel (refereegranskat)abstract
- The hottest developments in the fields of cancer research, neuroscience, genomics and proteomics, anti-virals and more, with a pick of the key research papers in these areas.
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| 10. |
- Spitzer, Eric, et al.
(författare)
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Monitor – biology
- 2004
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Ingår i: Drug Discovery Today. - 1878-5832. ; 9:21, s. 941-943
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Tidskriftsartikel (refereegranskat)abstract
- The hottest developments in the fields of cancer research, neuroscience, genomics and proteomics, anti-virals and more, with a pick of the key research papers in these areas.
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