| 1. |
- Ohlsson, Sophie, et al.
(författare)
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Novel distribution of the secretory leucocyte proteinase inhibitor in kidney
- 2001
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Ingår i: Mediators of Inflammation. - : Hindawi Limited. - 0962-9351 .- 1466-1861. ; 10:6, s. 347-350
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Tidskriftsartikel (refereegranskat)abstract
- The secretory leucocyte proteinase inhibitor (SLPI) is a low molecular weight, tissue-specific inhibitor of, for example, elastase and cathepsin G, which also have antimicrobial capacity. SLPI has been localised to the respiratory, gastrointestinal and genital tracts, but so far not to the kidney. The presence of SLPI in renal tubuli cells was demonstrated using immunohistochemistry and, by means of in situ hybridisation on human renal biopsies, we were able to demonstrate SLPI production. In various inflammatory conditions in the kidneys, the protease-antiprotease balance is disturbed. For this reason, as well as the possible role in the defence against ascending urinary tract infections, it is interesting to establish a source of SLPI in renal tubuli cells.
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| 2. |
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| 3. |
- Bergh, Anders, et al.
(författare)
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Didecyl squarate — A practical amino-reactive cross-linking reagent for neoglycoconjugate synthesis
- 2001
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Ingår i: Glycoconjugate Journal. - 1573-4986. ; 18:8, s. 615-621
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Tidskriftsartikel (refereegranskat)abstract
- The present paper describes the synthesis and use of the hydrophobic squaric decyl ester glycosides in neoglycoconjugate chemistry. The 2-aminoethyl glycosides of agr-D-mannopyranose, lactose, globotriose, globotetraose, GM3, and sialyl Lewisx, as well as the 2-(2-aminoethylthio)ethyl glycoside of agr-D-mannopyranose, beta-D-glucopyranose, and galabiose were reacted with squaric acid didecyl ester to afford the hydrophobic squaric decyl ester glycosides. These glycosides were efficient reagents for the conjugation to amino-functional microtiter plates, BSA and aminated Sepharose EAH 4B. The decyl ester moiety of the squaric decyl ester glycosides constitutes a traceless hydrophobic tag, which has the major advantage, as compared to the corresponding ethyl esters, that it enables easy purification of the glycosides with silica chromatography and that unreacted excesses glycosides from conjugation reaction mixtures can easily be recovered by means of C18 solid phase extraction.
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| 4. |
- Björnham, Oscar, 1976-, et al.
(författare)
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Measurements of the binding force between the Helicobacter pylori adhesin BabA and the Lewis b blood group antigen using optical tweezers
- 2005
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Ingår i: Journal of Biomedical Optics. - Bellingham, WA : SPIE-Intl Soc Optical Eng. - 1083-3668 .- 1560-2281. ; 10:4, s. 044024-044032
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Tidskriftsartikel (refereegranskat)abstract
- Helicobacter pylori is a world-wide spread bacterium that causes persistent infections and chronic inflammations that can develop into gastritis and peptic ulcer disease. It expresses several adhesin proteins on its surface that bind to specific receptors in the gastric epithelium. The most well-known adhesin is BabA, which has previously been shown to bind specifically to the fucosylated blood group antigen Lewis b (Leb). The adhesion forces between BabA and the Leb antigen are investigated in this work and assessed by means of optical tweezers. A model system for in situ measurements of the interaction forces between individual bacteria and beads coated with Leb is developed. It is found that the de-adhesion force in this model system, measured with a loading rate of ~100 pN/s, ranges from 20 to 200 pN. The de-adhesion force appears predominantly as multiples of an elementary force, which is determined to 25±1.5 pN and identified as the unbinding force of an individual BabA-Leb binding. It is concluded that adhesion in general is mediated by a small number of bindings (most often 1 to 4) despite that the contact surface between the bacterium and the bead encompassed significantly more binding sites.
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| 5. |
- Ellervik, Ulf, et al.
(författare)
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2-Bromoethyl glycosides for synthesis of glycoconjugates on solid support
- 2005
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Ingår i: Tetrahedron. - : Elsevier BV. - 0040-4020. ; 61:9, s. 2421-2429
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Tidskriftsartikel (refereegranskat)abstract
- 2-Bromoethyl glycosides can easily and in high yields be transformed into sulfones by treatment with a suitable thiol followed by oxidation with mCPBA. The observation that the so formed sulfones were cleaved by treatment with NaOMe/MeOH was used to design a new safety catch linker for synthesis of glycoconjugates on solid support. (C) 2005 Elsevier Ltd. All rights reserved.
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| 6. |
- Flygare, Lennart, et al.
(författare)
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Chronic recurrent multifocal osteomyelitis involving both jaws : report of a case including magnetic resonance correlation
- 1997
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Ingår i: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics. - : Elsevier. - 1079-2104 .- 1528-395X. ; 83:2, s. 300-305
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Tidskriftsartikel (refereegranskat)abstract
- A case of chronic recurrent multifocal osteomyelitis in a 14-year-old girl is presented. The disease had an initial aggressive osteolytic appearance involving both the maxilla and the mandible. Conservative treatment with minimal surgical intervention has been successful in this case during a 2-year follow-up period. The value of magnetic resonance imaging and bone scintigraphy in this case and the cause of chronic recurrent multifocal osteomyelitis is discussed.
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| 7. |
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| 8. |
- Hanson, Jörgen, et al.
(författare)
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P2PIE : a New Enterprise Application Integration Solution 60-70
- 2015
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Ingår i: Proceedings of the CAiSE-2015 Industry Track co-located with 27th Conference on Advanced Information Systems Engineering (CAiSE 2015). ; , s. 60-70
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Konferensbidrag (refereegranskat)abstract
- In order to provide unified and consistent data and functionality, enterprises need to make all their applications work in an integrated manner. However, integration has become more difficult to handle when enterprises extend their business globally, more applications are implemented for supporting business activities, and more computing paradigms are adopted, e.g., cloud, social, and mobile, just to name a few. The state-of-the-art integration solutions have limitations to address the difficulty. In this paper, point to point integration engine-P2PIE, a new integration solution is presented and discussed. The solution combines distributed execution of integration with centralized management console, which innovate the fundamental integration infrastructures of point to point paradigm, and message systems. P2PIE is developed and tested by Sandvik over the decade. The solution has addressed Sandvik’s challenges in enterprise application integration. Agility, cost reduction and reliability of this solution are proved.
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| 9. |
- Hellman, Karin, et al.
(författare)
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Discovery of Procognitive Antipsychotics by Combining Muscarinic M-1 Receptor Structure-Activity Relationship with Systems Response Profiles in Zebrafish Larvae
- 2020
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Ingår i: ACS Chemical Neuroscience. - : American Chemical Society (ACS). - 1948-7193. ; 11:2, s. 173-183
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Tidskriftsartikel (refereegranskat)abstract
- Current antipsychotic drugs are notably ineffective at addressing the cognitive deficits associated with schizophrenia. N-Desmethylclozapine (NDMC), the major metabolite of clozapine, displays muscarinic M-1 receptor (M-1) agonism, an activity associated with improvement in cognitive functioning. Preclinical and clinical data support that M-1 agonism may be a desired activity in antipsychotic drugs. However, NDMC failed clinical phase II studies in acute psychotic patients. NDMC analogues were synthesized to establish a structure-activity relationship (SAR) at the M-1 receptor as an indication of potential procognitive properties. In vitro evaluation revealed a narrow SAR in which M-1 agonist activity was established by functionalization in the 4- and 8-positions in the tricyclic core. In vivo behavioral response profiles were used to evaluate antipsychotic efficacy and exposure in zebrafish larvae and peripheral side effect related M-1 activity in adult zebrafish. The NDMC analogue 13f demonstrated antipsychotic activity similar to clozapine including M-1 agonist activity. Cotreatment with trospium chloride, an M1 peripheral acting antagonist, counteracted peripheral side effects. Thus, the NDMC analogue 13f, in combination with a peripherally acting anticholinergic compound, could be suitable for further development as an antipsychotic compound with potential procognitive activity.
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| 10. |
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