| 1. |
- Andersson, Mariette, et al.
(författare)
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Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery
- 2018
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Ingår i: Physiologia Plantarum. - : Wiley. - 0031-9317 .- 1399-3054. ; 164, s. 378-384
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Tidskriftsartikel (refereegranskat)abstract
- Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum). From both a scientific and a regulatory perspective, it is beneficial if integration of DNA in the potato genome is avoided. We have implemented a DNA-free genome editing method, using delivery of CRISPR-Cas9 ribonucleoproteins (RNPs) to potato protoplasts, by targeting the gene encoding a granule bound starch synthase (GBSS, EC 2.4.1.242). The RNP method was directly implemented using previously developed protoplast isolation, transfection and regeneration protocols without further adjustments. Cas9 protein was preassembled with RNA produced either synthetically or by in vitro transcription. RNP with synthetically produced RNA (cr-RNP) induced mutations, i.e. indels, at a frequency of up to 9%, with all mutated lines being transgene-free. A mutagenesis frequency of 25% of all regenerated shoots was found when using RNP with in vitro transcriptionally produced RNA (IVT-RNP). However, more than 80% of the shoots with confirmed mutations had unintended inserts in the cut site, which was in the same range as when using DNA delivery. The inserts originated both from DNA template remnants from the in vitro transcription, and from chromosomal potato DNA. In 2-3% of the regenerated shoots from the RNP-experiments, mutations were induced in all four alleles resulting in a complete knockout of the GBSS enzyme function.
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| 2. |
- Strauss, Leena, et al.
(författare)
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Seminal vesicles and urinary bladder as sites of aromatization of androgens in men, evidenced by a CYP19A1-driven luciferase reporter mouse and human tissue specimens.
- 2013
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Ingår i: The FASEB Journal. - : Wiley. - 0892-6638 .- 1530-6860. ; 27:4, s. 1342-50
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Tidskriftsartikel (refereegranskat)abstract
- The human CYP19A1 gene is expressed in various tissues by the use of tissue-specific promoters, whereas the rodent cyp19a1 gene is expressed mainly in the gonads and brain. We generated a transgenic mouse model containing a >100-kb 5' region of human CYP19A1 gene connected to a luciferase reporter gene. The luciferase activity in mouse tissues mimicked the CYP19A1 gene expression pattern in humans. Interestingly, the reporter gene activity was 16 and 160 times higher in the urinary bladder and seminal vesicles, respectively, as compared with the activity in the testis. Accordingly, CYP19A1 gene and P450arom protein expression was detected in those human tissues. Moreover, the data revealed that the expression of CYP19A1 gene is driven by promoters PII, I.4, and I.3 in the seminal vesicles, and by promoters PII and I.4 in the urinary bladder. Furthermore, the reporter gene expression in the seminal vesicles was androgen dependent: Castration decreased the expression ∼20 times, and testosterone treatment restored it to the level of an intact mouse. This reporter mouse model facilitates studies of tissue-specific regulation of the human CYP19A1 gene, and our data provide evidence for seminal vesicles as important sites for estrogen production in males.-Strauss, L., Rantakari, P., Sjögren, K., Salminen, A., Lauren, E., Kallio, J., Damdimopoulou, P., Boström, M., Boström, P. J., Pakarinen, P., Zhang, F. P., Kujala, P., Ohlsson, C., Mäkelä, S., Poutanen, M. Seminal vesicles and urinary bladder as sites of aromatization of androgens in men, evidenced by a CYP19A1-driven luciferase reporter mouse and human tissue specimens.
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| 3. |
- Tetler, Susan, et al.
(författare)
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Från idé till praxis : vägar till inkluderande lärmiljöer i tolv svenska kommuner. Forskarnas rapport 2015:2
- 2015
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Denna forskningsrapport utgår ifrån det treåriga FoU-programmet ”Inkluderande lärmiljöer” som startade hösten 2012 med 31 deltagande grundskolor fördelade på tolv kommuner. Malmö högskola har sedan programmets start följt de skolutvecklingsprocesser som skett på flera nivåer: 1) på kommunal nivå, där de tolv kommunernas förvaltningschefer och inkluderingskoordinatorer varit en drivande kraft i processen med att utveckla inkluderande lärmiljöer i deras respektive kommuner, 2) på institutionell nivå, där det primärt varit fokus på hur inkluderande ledarskap avspeglats i programmet med utgångspunkt i de deltagande skolornas rektorer, och 3) skolornas lärmiljöer, där de konkreta pedagogiska och didaktiska anpassningar utvecklats och implementerats, för att skapa förutsättningar för delaktighet och goda villkor för alla barns lärande. Malmö högskolas forskning som följt projektet kan karaktäriseras som en ”multi-site” och ett ”multi-researcher”-projekt, som byggt på ett dialogiskt och respektfullt samarbete med FoU-programmets övriga parter: aktörer från de tolv kommunerna och 31 skolorna, Ifous (Innovation, forskning och utveckling i skola och förskola) samt SPSM (Specialpedagogiska skolmyndigheten).
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| 4. |
- Tetler, Susan, et al.
(författare)
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Från idé till praxis : vägar till inkluderande lärmiljöer i tolv svenska kommuner. Forskarnas rapport 2015:2
- 2015
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Denna forskningsrapport utgår ifrån det treåriga FoU-programmet ”Inkluderande lärmiljöer” som startade hösten 2012 med 31 deltagande grundskolor fördelade på tolv kommuner. Malmö högskola har sedan programmets start följt de skolutvecklingsprocesser som skett på flera nivåer: 1) på kommunal nivå, där de tolv kommunernas förvaltningschefer och inkluderingskoordinatorer varit en drivande kraft i processen med att utveckla inkluderande lärmiljöer i deras respektive kommuner, 2) på institutionell nivå, där det primärt varit fokus på hur inkluderande ledarskap avspeglats i programmet med utgångspunkt i de deltagande skolornas rektorer, och 3) skolornas lärmiljöer, där de konkreta pedagogiska och didaktiska anpassningar utvecklats och implementerats, för att skapa förutsättningar för delaktighet och goda villkor för alla barns lärande. Malmö högskolas forskning som följt projektet kan karaktäriseras som en ”multi-site” och ett ”multi-researcher”-projekt, som byggt på ett dialogiskt och respektfullt samarbete med FoU-programmets övriga parter: aktörer från de tolv kommunerna och 31 skolorna, Ifous (Innovation, forskning och utveckling i skola och förskola) samt SPSM (Specialpedagogiska skolmyndigheten).
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| 5. |
- Tordsson, Jesper, et al.
(författare)
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A3 -- a novel colon and pancreatic cancer reactive antibody from a primate phage library selected using intact tumour cells
- 2000
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Ingår i: International Journal of Cancer. - 0020-7136. ; 87:4, s. 559-568
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Tidskriftsartikel (refereegranskat)abstract
- The identification of novel tumour-associated antigens (TAAs) is pivotal for progression in the fields of tumour immunotherapy and diagnosis. In the present study, we have developed, based on flow cytometric evaluation and use of a mini-library composed of specific antibody clones linked to different antibiotic resistance markers, methods for positive and subtractive selection of phage antibodies employing intact cells as the antigen source. An scFv phage library (2.7 x 10(7)) was constructed from a primate (Macaca fascicularis) immunised with pooled human colon carcinomas. This library was selected for 3 rounds by binding to Colo 205 colon adenocarcinoma cells and proteolytic elution followed by phage amplification. Several antibodies reactive with colon carcinomas and with restricted reactivity to a few epithelial normal tissues were identified by immunohistochemistry. One clone, A3 scFv, recognised an epitope that was homogeneously expressed in 11/11 of colon and 4/4 pancreatic carcinomas studied and in normal tissue restricted to subtypes of epithelia in the gastrointestinal tract. The A3 scFv had an apparent overall affinity approximately 100-fold higher than an A3 Fab, suggesting binding of scFv homodimers. The cell surface density of the A3 epitope, calculated on the basis of Fab binding, was exceptionally high, approaching 3 million per cell. We also demonstrate efficient T-cell-mediated killing of colon cancer cells coated with A3 scFv fused to the low MHC class II binding superantigen mutant SEA(D227A). The identified A3 molecule thus represents a TAA with properties that suggest its use for immunotherapy of colon and pancreatic cancer.
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| 6. |
- Westergren, Albert, et al.
(författare)
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Eating difficulties, need for assisted eating, nutritional status and pressure ulcers in patients admitted for geriatric stroke rehabilitation
- 2001
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Ingår i: Journal of Clinical Nursing. - 0962-1067 .- 1365-2702. ; 10:2, s. 257-269
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to describe the types and extent of eating difficulties, the need for assistance when eating, the nutritional status and pressure ulcers in consecutive patients (n = 162) admitted for stroke rehabilitation over a period of 1 year. Structured observations and assessments of eating, nutritional status (subjective global assessment of nutritional status), pressure ulcers and activities in daily living (Katz ADL-index) were performed by a nurse who also trained the staff to perform these assessments. Difficulties in eating were found in 80%, and 52.5% were unable to eat without assistance. Eating difficulties were: 'eats three-quarters or less of served food' (60%), difficulties in 'manipulating food on the plate' (56%), 'transportation of food to the mouth' (46%), 'sitting position' (29%), 'aberrant eating speed' (slow or forced) (26%), 'manipulating food in the mouth' (leakage, hoarding, chewing difficulties) (24%), 'swallowing difficulties' (18%), 'opening and/or closing the mouth' (16%), and 'alertness' (9%). Thirty-two percent were undernourished (49% of patients needing assisted eating and 13% of those not needing assistance, P < 0.0005). Among patients who were dependent in one or more functions according to the Katz ADL-index, 15% had pressure ulcers. The strongest eating variables for predicting nutritional status were 'alertness', 'swallowing difficulties', 'eats three-quarters or less of served food', and 'aberrant eating speed'. Nutritional status could in turn significantly predict pressure ulcers. Eating difficulties among patients with stroke are complex and the patient's situation before stroke adds to this complexity, especially among those dependent on assisted eating. As difficulties occur both among patients needing and not needing assisted eating, all patients with stroke admitted for rehabilitation need to be systematically assessed for eating difficulties and action needs to be taken to facilitate eating, especially as patients with eating difficulties risk becoming undernourished and in turn developing pressure ulcers.
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| 7. |
- Westergren, Albert, et al.
(författare)
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Eating difficulties, need for assisted eating, nutritional status and pressure ulcers in patients admitted for stroke rehabilitation
- 2001
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Ingår i: Journal of Clinical Nursing. - : Wiley. - 1365-2702 .- 0962-1067. ; 10:2, s. 257-269
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to describe the types and extent of eating difficulties, the need for assistance when eating, the nutritional status and pressure ulcers in consecutive patients (n = 162) admitted for stroke rehabilitation over a period of 1 year. Structured observations and assessments of eating, nutritional status (subjective global assessment of nutritional status), pressure ulcers and activities in daily living (Katz ADL-index) were performed by a nurse who also trained the staff to perform these assessments. Difficulties in eating were found in 80%, and 52.5% were unable to eat without assistance. Eating difficulties were: 'eats three-quarters or less of served food' (60%), difficulties in 'manipulating food on the plate' (56%), 'transportation of food to the mouth' (46%), 'sitting position' (29%), 'aberrant eating speed' (slow or forced) (26%), 'manipulating food in the mouth' (leakage, hoarding, chewing difficulties) (24%), 'swallowing difficulties' (18%), 'opening and/or closing the mouth' (16%), and 'alertness' (9%). Thirty-two percent were undernourished (49% of patients needing assisted eating and 13% of those not needing assistance, P < 0.0005). Among patients who were dependent in one or more functions according to the Katz ADL-index, 15% had pressure ulcers. The strongest eating variables for predicting nutritional status were 'alertness', 'swallowing difficulties', 'eats three-quarters or less of served food', and 'aberrant eating speed'. Nutritional status could in turn significantly predict pressure ulcers. Eating difficulties among patients with stroke are complex and the patient's situation before stroke adds to this complexity, especially among those dependent on assisted eating. As difficulties occur both among patients needing and not needing assisted eating, all patients with stroke admitted for rehabilitation need to be systematically assessed for eating difficulties and action needs to be taken to facilitate eating, especially as patients with eating difficulties risk becoming undernourished and in turn developing pressure ulcers.
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