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Sökning: WFRF:(Ohta Ryoichi)

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1.
  • Ohta, Ryoichi, et al. (författare)
  • Relationship between bonding strength and surface roughness in low-temperature bonding of glass for micro/nanofluidic device
  • 2024
  • Ingår i: Journal of Micromechanics and Microengineering. - 0960-1317. ; 34:1
  • Tidskriftsartikel (refereegranskat)abstract
    • The bonding of glass substrates is an important process in the fabrication of glass micro/nanofluidic devices. In this study, the influence of the surface roughness of glass substrates after low-temperature bonding is investigated. It is found that plasma etching can be used to control the surface roughness to the range 2-9 nm. Substrates with a roughness of 5 nm or less can be bonded. The pressure capacity of devices tends to decrease with increasing surface roughness. A pressure capacity of 500 kPa or higher is obtained with a surface roughness of 2 nm or less. This criterion for bonding conditions can be applied to roughness formed by other methods (e.g. via a Cr layer). The proposed approach will facilitate the design and fabrication of glass micro/nanofluidic devices, especially those that complicated fabrication processes or embedding of multiple materials.
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2.
  • Sano, Hiroki, et al. (författare)
  • Nanofluidic analytical system integrated with nanochannel open/close valves for enzyme-linked immunosorbent assay
  • 2023
  • Ingår i: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 23, s. 727-736
  • Tidskriftsartikel (refereegranskat)abstract
    • There have been significant advances in the field of nanofluidics, and novel technologies such as single-cell analysis have been demonstrated. Despite the evident advantages of nanofluidics, fluid control in nanochannels for complicated analyses is extremely difficult because the fluids are currently manipulated by maintaining the balance of driving pressure. To address this issue, the use of valves will be essential. Our group previously developed a nanochannel open/close valve utilizing glass deformation, but this has not yet been integrated into nanofluidic devices for analytical applications. In the present study, a nanofluidic analytical system integrated with multiple nanochannel open/close valves was developed. This system consists of eight pneumatic pumps, seven nanochannel open/close valves combined with piezoelectric actuators, and an ultra-high sensitivity detector for non-fluorescent molecules. For simultaneous actuation of multiple valves, a device holder was designed that prevented deformation of the entire device caused by operating the valves. A system was subsequently devised to align each valve and actuator with a precision of better than 20 μm to permit the operation of valves. The developed analytical system was verified by analyzing IL-6 molecules using an enzyme-linked immunosorbent assay. Fluid operations such as sample injection, pL-level aliquot sampling and flow switching were accomplished in this device simply by opening/closing specific valves, and a sample consisting of approximately 1500 IL-6 molecules was successfully detected. This study is expected to significantly improve the usability of nanofluidic analytical devices and lead to the realization of sophisticated analytical techniques such as single-cell proteomics.
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3.
  • Smirnova, Adelina, et al. (författare)
  • Enzyme-linked immunosorbent assay using thin-layered microfluidics with perfect capture of the target protein
  • 2023
  • Ingår i: Analytical Methods. - : Royal Society of Chemistry (RSC). - 1759-9660 .- 1759-9679. ; 15:5, s. 675-684
  • Tidskriftsartikel (refereegranskat)abstract
    • We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. This channel possesses a high aspect ratio (width/depth ∼200) and has an antibody layer immobilized directly on the channel surface. A depth of several microns and an excessive width and length (mm scale) of the channel provide a large-volume capacity (102 nL) and maximum capture efficiency of the analyte for a high level of detection sensitivity (102 pg mL−1). The developed reusable immunosensor has demonstrated high-performance characteristics by requiring less than 50 μL of sample and providing analysis in less than 25 min. This new method could impact the development of point-of-care devices for biomedical applications.
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