| 1. |
- Cossarizza, A., et al.
(författare)
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
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Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
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Tidskriftsartikel (refereegranskat)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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| 2. |
- Polme, S., et al.
(författare)
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FungalTraits: a user-friendly traits database of fungi and fungus-like stramenopiles
- 2020
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Ingår i: Fungal Diversity. - : Springer Science and Business Media LLC. - 1560-2745 .- 1878-9129. ; 105:1, s. 1-16
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Tidskriftsartikel (refereegranskat)abstract
- The cryptic lifestyle of most fungi necessitates molecular identification of the guild in environmental studies. Over the past decades, rapid development and affordability of molecular tools have tremendously improved insights of the fungal diversity in all ecosystems and habitats. Yet, in spite of the progress of molecular methods, knowledge about functional properties of the fungal taxa is vague and interpretation of environmental studies in an ecologically meaningful manner remains challenging. In order to facilitate functional assignments and ecological interpretation of environmental studies we introduce a user friendly traits and character database FungalTraits operating at genus and species hypothesis levels. Combining the information from previous efforts such as FUNGuild and Fun(Fun) together with involvement of expert knowledge, we reannotated 10,210 and 151 fungal and Stramenopila genera, respectively. This resulted in a stand-alone spreadsheet dataset covering 17 lifestyle related traits of fungal and Stramenopila genera, designed for rapid functional assignments of environmental studies. In order to assign the trait states to fungal species hypotheses, the scientific community of experts manually categorised and assigned available trait information to 697,413 fungal ITS sequences. On the basis of those sequences we were able to summarise trait and host information into 92,623 fungal species hypotheses at 1% dissimilarity threshold.
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| 3. |
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| 4. |
- Erikson, A, et al.
(författare)
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The near-Earth objects follow-up program - III. 3 lightcurves for 12 objects from 1992 and 1995
- 2000
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Ingår i: ICARUS. - : ACADEMIC PRESS INC. - 0019-1035. ; 147:2, s. 487-497
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Tidskriftsartikel (refereegranskat)abstract
- In this paper we present lightcurves and physical parameters for nine near-Earth objects (NEOs) (433 Eros, 1917 Cuyo, 2368 Beltrovata, 3753 Cruithne, 4769 Castalia, 4953 1990 MU, 6491 1991 OA, 1995 EK1, and 1995 FJ) and three Mars crossers (5738 Billpicke
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| 5. |
- Piironen, J, et al.
(författare)
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Physical studies of asteroids - XXXII. Rotation periods and UBVRI-colours for selected asteroids
- 1998
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Ingår i: ASTRONOMY & ASTROPHYSICS SUPPLEMENT SERIES. - : E D P SCIENCES. - 0365-0138. ; 128:3, s. 525-540
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- We present lightcurves of selected asteroids. Most of the asteroids were included to obtain refined spin periods. Enhanced periods were determined for 11 Parthenope, 306 Unitas and 372 Palma. We confirmed the spin periods of 8 Flora, 13 Egeria, 71 Niobe,
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| 6. |
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| 7. |
- Huber, Johannes A. J., 1989-, et al.
(författare)
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A method for generating finite element models of wood boards from X-ray computed tomography scans
- 2022
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Ingår i: Computers & structures. - : Elsevier. - 0045-7949 .- 1879-2243. ; 260
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Tidskriftsartikel (refereegranskat)abstract
- A method is presented for reconstructing the geometry, the pith, the knots and the local fibre orientations in timber boards, based on X-ray computed tomography scans. The local fibre deviations around knots were found by a new algorithm, based on image analysis. The experimental data comprised tomography scans, eigenfrequency measurements and four-point bending tests of 20 Norway spruce boards. 3D and 1D finite element models of the pure bending zone of the bending tests were created, accounting for the exact board geometry and the reconstructed fibre deviations. A purely density based, a purely eigenfrequency based, and a mixed constitutive law were compared. Model estimations showed a high coefficient of determination (R2) for global modulus of elasticity (MoE) (R2⩽0.93), local MoE (R2⩽0.87), bending strength (R2⩽0.83), and the location of initial failure. Constitutive laws accounting for eigenfrequency showed the most accurate results. In the future, adapting the method for logs could enable analyses of boards before sawing.
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| 8. |
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| 9. |
- Kõljalg, Urmas, et al.
(författare)
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Towards a unified paradigm for sequence-based identification of fungi.
- 2013
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Ingår i: Molecular ecology. - : Wiley. - 1365-294X .- 0962-1083. ; 22:21, s. 5271-7
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Tidskriftsartikel (refereegranskat)abstract
- The nuclear ribosomal internal transcribed spacer (ITS) region is the formal fungal barcode and in most cases the marker of choice for the exploration of fungal diversity in environmental samples. Two problems are particularly acute in the pursuit of satisfactory taxonomic assignment of newly generated ITS sequences: (i) the lack of an inclusive, reliable public reference data set and (ii) the lack of means to refer to fungal species, for which no Latin name is available in a standardized stable way. Here, we report on progress in these regards through further development of the UNITE database (http://unite.ut.ee) for molecular identification of fungi. All fungal species represented by at least two ITS sequences in the international nucleotide sequence databases are now given a unique, stable name of the accession number type (e.g. Hymenoscyphus pseudoalbidus|GU586904|SH133781.05FU), and their taxonomic and ecological annotations were corrected as far as possible through a distributed, third-party annotation effort. We introduce the term 'species hypothesis' (SH) for the taxa discovered in clustering on different similarity thresholds (97-99%). An automatically or manually designated sequence is chosen to represent each such SH. These reference sequences are released (http://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and in the QIIME pipeline. The system and the data will be updated automatically as the number of public fungal ITS sequences grows. We invite everybody in the position to improve the annotation or metadata associated with their particular fungal lineages of expertise to do so through the new Web-based sequence management system in UNITE.
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| 10. |
- LAGERKVIST, CI, et al.
(författare)
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PHYSICAL STUDIES OF ASTEROIDS .29. PHOTOMETRY AND ANALYSIS OF 27 ASTEROIDS
- 1995
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Ingår i: ASTRONOMY & ASTROPHYSICS SUPPLEMENT SERIES. - : EDITIONS PHYSIQUE. - 0365-0138. ; 113:1, s. 115-129
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Tidskriftsartikel (refereegranskat)abstract
- In this paper photometry and analysis of 27 asteroids are presented. For the asteroids 21 Lutetia, 23 Thalia, 250 Bettina and 135 Hertha spin vector determinations were made. For asteroid 99 Dike we derived a rotation period of 10.35 hours and for 1280 Ba
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