| 1. |
- Doi, Daisuke, et al.
(författare)
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Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation.
- 2014
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Ingår i: Stem Cell Reports. - : Elsevier BV. - 2213-6711. ; 2:3, s. 337-350
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Tidskriftsartikel (refereegranskat)abstract
- Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson's disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN(+) cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN(+) cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor behavior, without tumor formation. In particular, the CORIN(+) cells in a NURR1(+) cell-dominant stage exhibited the best survival and function as DA neurons. Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application.
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| 2. |
- Hashimoto, Takuya, et al.
(författare)
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Big Three Dragons: A z=7.15 Lyman-break galaxy detected in [Oiii] 88 mu m, [Cii] 158 mu m, and dust continuum with ALMA
- 2019
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Ingår i: Publication of the Astronomical Society of Japan. - : Oxford University Press (OUP). - 2053-051X .- 0004-6264. ; 71:4
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Tidskriftsartikel (refereegranskat)abstract
- We present new ALMA observations and physical properties of a Lyman break galaxy at z = 7.15. Our target, B14-65666, has a bright ultra-violet (UV) absolute magnitude, M-UV approximate to -22.4, and has been spectroscopically identified in Ly alpha with a small rest-frame equivalent width of approximate to 4 angstrom. A previous Hubble Space TElescope (HST) image has shown that the target is composed of two spatially separated clumps in the rest-frame UV. With ALMA, we have newly detected spatially resolved [Oiii] 88 mu m, [Cii] 158 mu m, and their underlying dust continuum emission. In the whole system of B14-65666, the [Oiii] and [Cii] lines have consistent redshifts of 7.1520 +/- 0.0003, and the [Oiii] luminosity, (34.4 +/- 4.1)x 10(8)L(circle dot), is about three times higher than the [Cii] luminosity, (11.0 +/- 1.4) x 10(8)L(circle dot). With our two continuum flux densities, the dust temperature is constrained to be T-d approximate to 50-60K under the assumption of a dust emissivity index of beta(d) = 2.0-1.5, leading to a large total infrared luminosity of L-TIR approximate to 1 x 10(12)L(circle dot). Owing to our high spatial resolution data, we show that the [Oiii] and [Cii] emission can be spatially decomposed into two clumps associated with the two rest-frame UV clumps whose spectra are kinematically separated by approximate to 200kms(-1). We also find these two clumps have comparable UV, infrared, [Oiii], and [Cii] luminosities. Based on these results, we argue that B14-65666 is a starburst galaxy induced by a major merger. The merger interpretation is also supported by the large specific star formation rate (defined as the star formation rate per unit stellar mass), sSFR Gyr(-1), inferred from our SED fitting. Probably, a strong UV radiation field caused by intense star formation contributes to its high dust temperature and the [Oiii]-to-[Cii] luminosity ratio.
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| 3. |
- Hashimoto, Takuya, et al.
(författare)
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Big Three Dragons: Molecular Gas in a Bright Lyman-break Galaxy at z = 7.15
- 2023
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Ingår i: Astrophysical Journal. - 1538-4357 .- 0004-637X. ; 952:1
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Tidskriftsartikel (refereegranskat)abstract
- We report Atacama Large Millimeter/submillimeter Array Band 3 observations of CO(6−5), CO(7−6), and [C i](2−1) in B14-65666 (“Big Three Dragons”), one of the brightest Lyman-break galaxies at z > 7 in the rest-frame ultraviolet continuum, far-infrared continuum, and emission lines of [O iii] 88 μm and [C ii] 158 μm. CO(6−5), CO(7−6), and [C i](2−1), whose 3σ upper limits on the luminosities are approximately 40 times fainter than the [C ii] luminosity, are all not detected. The L [C II]/L CO(6-5) and L [C II]/L CO(7-6) ratios are higher than the typical ratios obtained in dusty star-forming galaxies or quasar host galaxies at similar redshifts, and they may suggest a lower gas density in the photodissociated region in B14-65666. By using the (1) [C ii] luminosity, (2) dust mass-to-gas mass ratio, and (3) a dynamical mass estimate, we find that the molecular gas mass (M mol) is (0.05-11) × 1010 M ⊙. This value is consistent with the upper limit inferred from the nondetection of mid-J CO and [C i](2−1). Despite the large uncertainty in M mol, we estimate a molecular gas-to-stellar mass ratio (μ gas) of 0.65-140 and a gas depletion time (τ dep) of 2.5-550 Myr; these values are broadly consistent with those of other high-redshift galaxies. B14-65666 could be an ancestor of a passive galaxy at z ≳ 4 if no gas is fueled from outside the galaxy.
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| 4. |
- Ikawa, Takahiro, et al.
(författare)
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Ridge reconstruction in damaged extraction sockets using tunnel beta-tricalcium phosphate blocks : A 6-month histological study in beagle dogs
- 2020
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Ingår i: Journal of Periodontal Research. - : John Wiley & Sons. - 0022-3484 .- 1600-0765. ; 55:4, s. 496-502
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Tidskriftsartikel (refereegranskat)abstract
- Objective The present study aimed to evaluate the histological outcome of tunnel beta-TCP blocks grafting in extraction sockets missing the buccal bone wall, after 6 months of healing. Background Tunnel beta-tricalcium phosphate (beta-TCP) blocks made of randomly organized tunnel-shaped beta-TCP ceramics appeared promising for alveolar ridge preservation in tooth extraction sockets missing the buccal bone, in a previous study in dogs, with a 2-month healing time. Methods In six beagle dogs, the maxillary first premolars were extracted and the buccal bone was surgically removed to create bone defects of 4 mm (mesio-distal) x 5 mm (apico-coronal) x 4 mm (bucco-palatal). Thus, extraction sockets missing the buccal bone plate were grated with tunnel beta-TCP blocks (test) or left empty for spontaneous healing (control). Histology/histomorphometry was performed after 6 months of healing. Results The horizontal bucco-palatal width of the alveolar ridge was significantly greater at test sites than at control sites. The amount of mineralized tissue was greater at test sites (57.8% +/- 11.1%) than at control sites (28.9% +/- 8.5%), while the amount of connective tissue was significantly greater at control sites (41.7% +/- 6.4%) than at test sites (19.6% +/- 9.2%). No significant difference was found between sites in terms of basic multicellular units and bone marrow. Residual beta-TCP at test sites was 5.8% +/- 3.2%. Conclusion Grafting with tunnel beta-TCP block significantly limited the resorption of the alveolar ridge at extraction sockets missing the buccal bone compared with sites left to heal spontaneously, even after 6-month follow-up.
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| 5. |
- Jönsson, Marie, et al.
(författare)
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Identification of Transplantable Dopamine Neuron Precursors at Different Stages of Midbrain Neurogenesis.
- 2009
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Ingår i: Experimental Neurology. - : Elsevier BV. - 0014-4886. ; 219, s. 341-354
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Tidskriftsartikel (refereegranskat)abstract
- Protocols used for generation of mesencephalic dopamine (mesDA) neurons from stem cells, or fetal brain tissue, invariably result in cell preparations that are highly mixed in composition, containing mesDA neuron precursors in various states of fate commitment and differentiation. For further optimisation and refinement of these procedures it is essential to determine the optimal stage of development and phenotypic characterisics of cells used for grafting. We have used fluorescence-activated cell sorting procedures to isolate mesDA precursors in defined stages of differentiation from mouse ventral mesencephalon (VM), at embryonic day 10.5 (E10.5), when the mesDA neuron domain consists of proliferative radial glia-like cells expressing the mesDA neuron determinant Lmx1a and the floor-plate marker Corin, and at E12.5, when the VM has expanded to comprise a mixture of proliferative progenitors, neuroblasts and young neurons. The sorted cells were transplanted to the striatum of 6-hydroxydopamine-lesioned rats. Results show that the Lmx1a/Corin-expressing ventricular zone progenitors, which are the source of mesDA neurons in grafts from E10.5 VM, had lost this capacity at E12.5. At this later stage all transplantable mesDA precursors resided in the intermediate zone as postmitotic Nurr1-expressing neuroblasts. The more differentiated, TH-expressing cells survived sorting and transplantation poorly. We also provide evidence that, during early mesDA neurogenesis, the progenitors for nigral mesDA neurons segregate to lateral parts of the Lmx1a-expressing domain and can be selectively isolated based on their level of Corin expression. These results have implications for current efforts to develop well-characterised stem-cell derived mesDA progenitor cell preparations for cell therapy.
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| 6. |
- Mitsuhashi, Ikki, et al.
(författare)
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SERENADE. II. An ALMA Multiband Dust Continuum Analysis of 28 Galaxies at 5
- 2024
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Ingår i: Astrophysical Journal. - 1538-4357 .- 0004-637X. ; 971:2
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Tidskriftsartikel (refereegranskat)abstract
- We present an analysis of the Atacama Large Millimeter-submillimeter Array (ALMA) multiband dust continuum observations for 28 spectroscopically confirmed bright Lyman break galaxies at 5 lt; z lt; 8. Our sample consists of 11 galaxies at z ∼ 6 newly observed in our ALMA program, which substantially increases the number of 5 lt; z lt; 8 galaxies with both rest-frame 88 and 158 μm continuum observations, allowing us to simultaneously measure the IR luminosity and dust temperature for a statistical sample of z ≳ 5 galaxies for the first time. We derive the relationship between the ultraviolet (UV) slope (β UV) and infrared excess (IRX) for the z ∼ 6 galaxies, and find a shallower IRX-β UV relation compared to the previous results at z ∼ 2-4. Based on the IRX-β UV relation consistent with our results and the β UV-M UV relation including fainter galaxies in the literature, we find a limited contribution of the dust-obscured star formation to the total star formation rate density, ∼30% at z ∼ 6. Our measurements of the dust temperature at z ∼ 6-7, T dust = 40.9 − 9.1 + 10.0 K on average, support a gentle increase of T dust from z = 0 to z ∼ 6-7. Using an analytic model with parameters consistent with recent James Webb Space Telescope results, we discuss that the observed redshift evolution of the dust temperature can be reproduced by an ∼0.6 dex decrease in the gas depletion timescale and ∼0.4 dex decrease in the metallicity. The variety of T dust observed at high redshifts can also be naturally explained by scatters around the star formation main sequence and average mass-metallicity relation including an extremely high dust temperature of T dust gt; 80 K observed in a galaxy at z = 8.3.
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| 7. |
- Yabuta, Hikaru, et al.
(författare)
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Macromolecular organic matter in samples of the asteroid (162173) Ryugu
- 2023
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Ingår i: Science. - : American Association for the Advancement of Science. - 0036-8075 .- 1095-9203. ; 379:6634
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Tidskriftsartikel (refereegranskat)abstract
- Samples of the carbonaceous asteroid (162173) Ryugu were collected and brought to Earth by the Hayabusa2 spacecraft. We investigated the macromolecular organic matter in Ryugu samples and found that it contains aromatic and aliphatic carbon, ketone, and carboxyl functional groups. The spectroscopic features of the organic matter are consistent with those in chemically primitive carbonaceous chondrite meteorites that experienced parent-body aqueous alteration (reactions with liquid water). The morphology of the organic carbon includes nanoglobules and diffuse carbon associated with phyllosilicate and carbonate minerals. Deuterium and/or nitrogen-15 enrichments indicate that the organic matter formed in a cold molecular cloud or the presolar nebula. The diversity of the organic matter indicates variable levels of aqueous alteration on Ryugus parent body.
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