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- Buijs, J, et al.
(författare)
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Conformational stability of adsorbed insulin studied with mass spectrometry and hydrogen exchange
- 1999
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Ingår i: ANALYTICAL CHEMISTRY. - : AMER CHEMICAL SOC. - 0003-2700. ; 71:15, s. 3219-3225
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- A new method is described for direct monitoring of the conformational stability of proteins that are physically adsorbed from solution onto a solid substrate. The adsorption-induced conformational changes of insulin are studied using a combination of hydr
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| 5. |
- Møller, M. H., et al.
(författare)
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Scandinavian SSAI clinical practice guideline on choice of inotropic agent for patients with acute circulatory failure
- 2018
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Ingår i: Acta Anaesthesiologica Scandinavica. - Hoboken : John Wiley & Sons. - 0001-5172 .- 1399-6576. ; 62:4, s. 420-450
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Forskningsöversikt (refereegranskat)abstract
- BACKGROUND: Adult critically ill patients often suffer from acute circulatory failure and those with low cardiac output may be treated with inotropic agents. The aim of this Scandinavian Society of Anaesthesiology and Intensive Care Medicine guideline was to present patient-important treatment recommendations on this topic.METHODS: This guideline was developed according to GRADE. We assessed the following subpopulations of patients with shock: (1) shock in general, (2) septic shock, (3) cardiogenic shock, (4) hypovolemic shock, (5) shock after cardiac surgery, and (6) other types of shock, including vasodilatory shock. We assessed patient-important outcome measures, including mortality and serious adverse reactions.RESULTS: For all patients, we suggest against the routine use of any inotropic agent, including dobutamine, as compared to placebo/no treatment (very low quality of evidence). For patients with shock in general, and in those with septic and other types of shock, we suggest using dobutamine rather than levosimendan or epinephrine (very low quality of evidence). For patients with cardiogenic shock and in those with shock after cardiac surgery, we suggest using dobutamine rather than milrinone (very low quality of evidence). For the other clinical questions, we refrained from giving any recommendations or suggestions.CONCLUSIONS: We suggest against the routine use of any inotropic agent in adult patients with shock. If used, we suggest using dobutamine rather than other inotropic agents for the majority of patients, however, the quality of evidence was very low, implying high uncertainty on the balance between the benefits and harms of inotropic agents.
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| 6. |
- Oscarsson, E., et al.
(författare)
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Commercial infant cereals contain high concentrations of endotoxins and viable Bacillus spp.
- 2020
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Ingår i: Food Control. - : Elsevier BV. - 0956-7135. ; 118
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Tidskriftsartikel (refereegranskat)abstract
- Background: Endotoxin may cause inflammation and increased intestinal permeability in infants with immature immune systems and gut microbiota. This study analysed the presence of endotoxin together with other potentially hazardous agents in commercial infant cereals. Methods: Four porridges and six milk cereal drinks bought in Sweden were analysed for the presence of endotoxins using Endosafe MCS. The quantity of viable bacteria was determined using conventional culturing methods and the isolates were identified with Sanger sequencing. Enterotoxin producing genes were analysed through multiplex PCR and levels of mycotoxins were studied using uHPLC MS/MS. Results: The endotoxin concentration ranged from 1400 to 24200 EU/g powder. Viable bacteria were found in the products, indicating survivability of Bacillus spp in dry powders. No traces of mycotoxins were found. Conclusion: This study indicates that substantial concentrations of endotoxins are present in powdered infant cereal-based foods. Furthermore, the amount of living bacteria ingested with some of the products was high.
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| 7. |
- Uhlig, E., et al.
(författare)
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The live bacterial load and microbiota composition of prepacked “ready-to-eat” leafy greens during household conditions, with special reference to E. coli
- 2022
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Ingår i: International Journal of Food Microbiology. - : Elsevier BV. - 0168-1605. ; 377
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Tidskriftsartikel (refereegranskat)abstract
- Ready-to-eat (RTE) leafy greens are popular products that unfortunately have been associated with numerous foodborne illness outbreaks. Since the influence of consumer practices is essential for their quality and safety, the objective of this study was to analyze the microbiota of RTE products throughout shelf life during simulated household conditions. Products from different companies were analyzed in terms of plate counts, and resealed and unopened packages were compared. High bacterial loads were found, up to a total plate count of 9.6 log10 CFU/g, and Enterobacteriaceae plate counts up to 6.0 CFU/g on the expiration date. The effect of consumer practice varied, thus no conclusions regarding resealed or unopened bags could be drawn. The tested products contained opportunistic pathogens, such as Enterobacter homaechei, Hafnia paralvei and Pantoea agglomerans. Amplicon sequencing revealed that the relative abundance of major taxonomic groups changed during shelf life; Pseudomonadaceae and Xanthomonadaceae decreased, while Flavobacteriaceae and Marinomonadaceae inceased. Inoculation with E. coli CCUG 29300T showed that the relative abundance of Escherichia-Shigella was lower on rocket than on other tested leafy greens. Inoculation with E. coli strain 921 indicate growth at the beginning of shelf-life time, while E. coli 731 increases at the end, seemingly able to adapt to cold storage conditions. The high levels of live microorganisms, the detection of opportunistic pathogens, and the ability of E. coli strains to grow at refrigeration temperature raise concerns and indicate that the shelf life may be shortened to achieve a safer product. Due to variations between products, further studies are needed to define how long the shelf-life of these products should be, to ensure a safe product even at the end of the shelf-life period.
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| 8. |
- Uhlig, E., et al.
(författare)
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Use of bacterial strains antagonistic to Escherichia coli for biocontrol of spinach : A field trial
- 2021
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Ingår i: Innovative Food Science and Emerging Technologies. - : Elsevier BV. - 1466-8564. ; 74
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Tidskriftsartikel (refereegranskat)abstract
- To counteract global food safety hazards related to raw consumption of ready-to-eat leafy vegetables, a method to improve bacterial status using antagonistic bacteria was studied under field conditions. This is the first study to identify potential Escherichia coli antagonists from the native microbiota on leafy green vegetables and evaluate their effect in an industrial field production setting. Bacterial strains were isolated from different types of leafy green vegetables and selected upon their effect against E. coli in vitro, and out of 295 tested bacterial strains, 37 showed an antagonistic effect. Four of those antagonistic strains were coated in separate treatments onto spinach seeds and planted in the field. Both seeds and plants were analyzed by Illumina MiSeq next generation sequencing (NGS), and it was seen that the microbiota of the plants contained lower relative abundance of plant and human pathogenic genera. Higher β-diversity was observed for the samples treated with Bacillus coagulans LMG P-32205 and B. coagulans LMG P-32206 compared to control, indicating that those strains have induced substantial changes in the native microbiota of the leaves. A reduction of Escherichia-Shigella was seen for two of the isolates (Pseudomonas cedrina LMG P-32207 and Pseudomonas punonenis LMG P-32204) as the seeds developed into plants. Seeds inoculated with two of the strains (B. coagulans LMG P-32205 and B. coagulans LMG P-32206) had increased levels of Lactobacillaceae, and treatment with B. coagulans LMG P-32206 resulted in lower levels of Pantoea (from 31.4 to 12.2%). These results encourage the usage of bacterial antagonists as part of a global solution to reduce the risk of human pathogens on leafy green vegetables.
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| 9. |
- Vukusic, J., et al.
(författare)
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MOVPE-grown GaInNAsVCSELs at 1.3 mu m with conventional mirror design approach
- 2003
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Ingår i: Electronics Letters. - : Institution of Engineering and Technology (IET). - 0013-5194 .- 1350-911X. ; 39:8, s. 662-664
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Tidskriftsartikel (refereegranskat)abstract
- 1.3 mum oxide confined GaInNAs VCSELs designed using the same design philosophy used for standard 850 nm VCSELs is presented. The VCSELs have doped mirrors, with graded and highly doped interfaces, and are fabricated using production-friendly procedures. Multimode VCSELs (I I mum oxide aperture) with an emission wavelength of 1287 nm have a threshold current of 3 mA and produce I mW of output power at 20degreesC. The maximum operating temperature is 95degreesC. Emission at 1303 nm with I mW of output power and a threshold current of 7 mA has been observed from VCSELs with a larger detuning between the gain peak and the cavity resonance.
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