| 1. |
|
|
| 2. |
- Ertel, Dominik, et al.
(författare)
-
Anisotropy Parameters for Two-Color Photoionization Phases in Randomly Oriented Molecules : Theory and Experiment in Methane and Deuteromethane
- 2024
-
Ingår i: Journal of Physical Chemistry A. - 1089-5639 .- 1520-5215. ; 128:9, s. 1685-1697
-
Tidskriftsartikel (refereegranskat)abstract
- We present combined theoretical and experimental work investigating the angle-resolved phases of the photoionization process driven by a two-color field consisting of an attosecond pulse train and an infrared pulse in an ensemble of randomly oriented molecules. We derive a general form for the two-color photoelectron (and time-delay) angular distribution valid also in the case of chiral molecules and when relative polarizations of the photons contributing to the attosecond photoelectron interferometer differ. We show a comparison between the experimental data and theoretical predictions in an ensemble of methane and deuteromethane molecules, discussing the effect of nuclear dynamics on the photoionization phases. Finally, we demonstrate that the oscillating component and the phase of the two-color signal can be fitted by using complex asymmetry parameters, in perfect analogy to the atomic case.
|
|
| 3. |
- Minzioni, Paolo, et al.
(författare)
-
Roadmap for optofluidics
- 2017
-
Ingår i: Journal of Optics. - : Institute of Physics (IOP). - 2040-8978 .- 2040-8986. ; 19
-
Tidskriftsartikel (refereegranskat)abstract
- Optofluidics, nominally the research area where optics and fluidics merge, is a relatively new research field and it is only in the last decade that there has been a large increase in the number of optofluidic applications, as well as in the number of research groups, devoted to the topic. Nowadays optofluidics applications include, without being limited to, lab-on-a-chip devices, fluid-based and controlled lenses, optical sensors for fluids and for suspended particles, biosensors, imaging tools, etc. The long list of potential optofluidics applications, which have been recently demonstrated, suggests that optofluidic technologies will become more and more common in everyday life in the future, causing a significant impact on many aspects of our society. A characteristic of this research field, deriving from both its interdisciplinary origin and applications, is that in order to develop suitable solutions a combination of a deep knowledge in different fields, ranging from materials science to photonics, from microfluidics to molecular biology and biophysics, is often required. As a direct consequence, also being able to understand the long-term evolution of optofluidics research is not easy. In this article, we report several expert contributions on different topics so as to provide guidance for young scientists. At the same time, we hope that this document will also prove useful for funding institutions and stakeholders to better understand the perspectives and opportunities offered by this research field.
|
|
| 4. |
- Paie, Petra, et al.
(författare)
-
Structured-light-sheet imaging in an integrated optofluidic platform
- 2023
-
Ingår i: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 24:1, s. 34-46
-
Tidskriftsartikel (refereegranskat)abstract
- Heterogeneity investigation at the single-cell level reveals morphological and phenotypic characteristics in cell populations. In clinical research, heterogeneity has important implications in the correct detection and interpretation of prognostic markers and in the analysis of patient-derived material. Among single-cell analysis, imaging flow cytometry allows combining information retrieved by single cell images with the throughput of fluidic platforms. Nevertheless, these techniques might fail in a comprehensive heterogeneity evaluation because of limited image resolution and bidimensional analysis. Light sheet fluorescence microscopy opened new ways to study in 3D the complexity of cellular functionality in samples ranging from single-cells to micro-tissues, with remarkably fast acquisition and low photo-toxicity. In addition, structured illumination microscopy has been applied to single-cell studies enhancing the resolution of imaging beyond the conventional diffraction limit. The combination of these techniques in a microfluidic environment, which permits automatic sample delivery and translation, would allow exhaustive investigation of cellular heterogeneity with high throughput image acquisition at high resolution. Here we propose an integrated optofluidic platform capable of performing structured light sheet imaging flow cytometry (SLS-IFC). The system encompasses a multicolor directional coupler equipped with a thermo-optic phase shifter, cylindrical lenses and a microfluidic network to generate and shift a patterned light sheet within a microchannel. The absence of moving parts allows a stable alignment and an automated fluorescence signal acquisition during the sample flow. The platform enables 3D imaging of an entire cell in about 1 s with a resolution enhancement capable of revealing sub-cellular features and sub-diffraction limit details. The combination of structured illumination and light sheet fluorescence microscopy in a microfluidic integrated platform enables high throughput super-resolution imaging.
|
|
| 5. |
- Volpe, Annalisa, et al.
(författare)
-
Fabrication and assembling of a microfluidic optical stretcher polymeric chip combining femtosecond laser and micro injection molding technologies
- 2017
-
Ingår i: Proceedings of SPIE, the International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. ; 10092
-
Tidskriftsartikel (refereegranskat)abstract
- Microfluidic optical stretchers are valuable optofluidic devices for studying single cell mechanical properties. These usually consist of a single microfluidic channel where cells, with dimensions ranging from 5 to 20 Όm are trapped and manipulated through optical forces induced by two counter-propagating laser beams. Recently, monolithic optical stretchers have been directly fabricated in fused silica by femtosecond laser micromachining (FLM). Such a technology allows writing in a single step in the substrate volume both the microfluidic channel and the optical waveguides with a high degree of precision and flexibility. However, this method is very slow and cannot be applied to cheaper materials like polymers. Therefore, novel technological platforms are needed to boost the production of such devices on a mass scale. In this work, we propose integration of FLM with micro-injection moulding (ΌIM) as a novel route towards the cost-effective and flexible manufacturing of polymeric Lab-on-a-Chip (LOC) devices. In particular, we have fabricated and assembled a polymethylmethacrylate (PMMA) microfluidic optical stretcher by exploiting firstly FLM to manufacture a metallic mould prototype with reconfigurable inserts. Afterwards, such mould was employed for the production, through ΌIM, of the two PMMA thin plates composing the device. The microchannel with reservoirs and lodgings for the optical fibers delivering the laser radiation for cell trapping were reproduced on one plate, while the other included access holes to the channel. The device was assembled by direct fs-laser welding, ensuring sealing of the channel and avoiding thermal deformation and/or contamination. © 2017 SPIE.
|
|
