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- Imanishi, T., et al.
(författare)
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Integrative annotation of 21,037 human genes validated by full-length cDNA clones
- 2004
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Ingår i: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 2:6, s. 856-875
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Tidskriftsartikel (refereegranskat)abstract
- The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, gene prediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the cDNA clones. We have developed a human gene database, called the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). It provides the following: integrative annotation of human genes, description of gene structures, details of novel alternative splicing isoforms, non-protein-coding RNAs, functional domains, subcellular localizations, metabolic pathways, predictions of protein three-dimensional structure, mapping of known single nucleotide polymorphisms (SNPs), identification of polymorphic microsatellite repeats within human genes, and comparative results with mouse full-length cDNAs. The H-InvDB analysis has shown that up to 4% of the human genome sequence (National Center for Biotechnology Information build 34 assembly) may contain misassembled or missing regions. We found that 6.5% of the human gene candidates (1,377 loci) did not have a good protein-coding open reading frame, of which 296 loci are strong candidates for non-protein-coding RNA genes. In addition, among 72,027 uniquely mapped SNPs and insertions/deletions localized within human genes, 13,215 nonsynonymous SNPs, 315 nonsense SNPs, and 452 indels occurred in coding regions. Together with 25 polymorphic microsatellite repeats present in coding regions, they may alter protein structure, causing phenotypic effects or resulting in disease. The H-InvDB platform represents a substantial contribution to resources needed for the exploration of human biology and pathology.
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| 2. |
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| 3. |
- Ishigami, G., et al.
(författare)
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Modeling of Flexible and Rigid Wheels for Exploration Rover on Rough Terrain
- 2011
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Ingår i: Proceedings of the 28th International Symposium on Space Technology and Science.
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Konferensbidrag (refereegranskat)abstract
- This paper presents a comprehensive wheel model that can quantitatively evaluate traction performance of flexible/rigid wheelsdriving on deformable terrain. The proposed model exploits a terramechanics-based approach with taking account of pressures generated by wheel elasticity as well as terrain stiffness. Deflection of a flexible wheel typically depends on a relative pressure between thewheel and terrain: the wheel will be significantly deformed on rigid terrain whereas it will be hardly deformed on soft terrain. Therefore, the wheel-terrain interaction in the proposed model is divided into three contact sections: wheel front section, wheel deflected(flat) section, and wheel rear section. The traction force of the wheel is obtained as an integral of normal and shear stresses generatedat each section. Simulation studies with varied wheel pressures, such as flexible, semi-flexible, and rigid wheels, are conducted tovalidate the proposed model. Also, traction performances of flexible/rigid wheels are compared based on a metric called tractiveefficiency. The comparison implies an optimal wheel pressure of flexible wheel for better traction performance.
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| 5. |
- Xu, S., et al.
(författare)
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A field-trial of on-demand integration of grid and innovative wavelength multicasting service over WSON for emerging frontier applications
- 2010
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Ingår i: 2010 14th Conference on Optical Network Design and Modeling, ONDM 2010. - 9783901882418
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- To enable the quick applications and services of new-born innovative optical network service (INS) in largescale high-end grid computing, we propose an overlay approach to integrate grid and emerging INS resources establishing the value-added optical grid network flexibly cross over multi-domain WSONs. In particular, we introduce an edge-mode approach which flexibly leverages new multifunction wavelength selective multicasting (WSM) at a special edge close to the core node. To integrate grid and innovative optical network technologies efficiently, we propose a unified platform - optical grid/INS network infrastructure (OGINI). Through field trial experiments over a developed metropolitan-scale wavelength switched optical network (WSON) test-bed, the feasibility of the proposed WSM scheme in the optical transmission systems and the totally automated value-added OGN constructions supported by the proposed OGINI have been verified successfully.
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