1. |
- Oprea, Tudor I, et al.
(författare)
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Unexplored therapeutic opportunities in the human genome
- 2018
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Ingår i: Nature Reviews Drug Discovery. - : Springer Science and Business Media LLC. - 1474-1776 .- 1474-1784. ; 17:5, s. 317-332
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Tidskriftsartikel (refereegranskat)abstract
- A large proportion of biomedical research and the development of therapeutics is focused on a small fraction of the human genome. In a strategic effort to map the knowledge gaps around proteins encoded by the human genome and to promote the exploration of currently understudied, but potentially druggable, proteins, the US National Institutes of Health launched the Illuminating the Druggable Genome (IDG) initiative in 2014. In this article, we discuss how the systematic collection and processing of a wide array of genomic, proteomic, chemical and disease-related resource data by the IDG Knowledge Management Center have enabled the development of evidence-based criteria for tracking the target development level (TDL) of human proteins, which indicates a substantial knowledge deficit for approximately one out of three proteins in the human proteome. We then present spotlights on the TDL categories as well as key drug target classes, including G protein-coupled receptors, protein kinases and ion channels, which illustrate the nature of the unexplored opportunities for biomedical research and therapeutic development.
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2. |
- Niimi, Jun, et al.
(författare)
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Aroma-taste interactions between a model cheese aroma and five basic tastes in solution
- 2014
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Ingår i: Food Quality and Preference. - : Elsevier BV. - 0950-3293 .- 1873-6343. ; 31:1
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Tidskriftsartikel (refereegranskat)abstract
- The flavour perception of cheese results from complex sensory interactions between tastes and aromas. Using a model cheese solution, this study investigated perceived interactions between each of five basic tastes and a cheese aroma mixture containing ten volatile compounds commonly found in cheese. The five tastes - sucrose (sweetness), sodium chloride (NaCl) (saltiness), monosodium glutamate (MSG) (umami), lactic acid (sourness), and caffeine (bitterness) - were individually mixed with cheese aroma in water using a 5 taste level (0.2 log series) by 3 aroma level (0.5 log series) design. Aroma controls with no added taste were also included. This resulted in 18 samples for each single taste-aroma combination. An additional 18 samples were produced using a mixture of all 5 tastes with the 3 aroma levels. A panel of trained assessors (n=10) evaluated cheese flavour intensity and taste intensity using 100 point line scales. Evaluation was carried out in duplicate, with samples grouped by taste type; 1 evaluation session per taste per replicate. Within type, order of presentation was balanced, and taste type order was randomised between replicates. Cheese flavour intensity was enhanced by sucrose and NaCl, while being suppressed by lactic acid. NaCl enhanced cheese flavour intensity the most at high aroma level, while lactic acid suppressed the most at low aroma level. When MSG level was increased, cheese flavour intensity was enhanced at both low and medium aroma levels, but was suppressed at the high aroma level. The greatest enhancement of cheese flavour intensity was found with the mixture of 5 tastes. Aroma significantly enhanced umami and bitterness, but did not enhance sweetness, saltiness, or sourness. This study showed that the perceived interaction between taste and cheese aroma depended on taste type and on the concentration levels of both taste type and aroma. The mixture of tastes was more effective at enhancing cheese flavour intensity than single tastes. This study provides knowledge that will underpin further study of taste-aroma interactions in a model cheese that aims to optimise cheese flavour intensity and character.
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3. |
- Niimi, Jun, et al.
(författare)
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Cheddar cheese taste can be reconstructed in solution using basic tastes
- 2014
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Ingår i: International Dairy Journal. - : Elsevier BV. - 0958-6946 .- 1879-0143. ; 34:1, s. 116-124
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Tidskriftsartikel (refereegranskat)abstract
- The taste of cheese contributes to flavour character directly and by cross-modal interactions with aroma. However, the relative contribution of specific tastes, i.e., sweet, salt, umami, sour, and bitter, is not well understood. Twelve cheeses were profiled by a trained sensory panel and the five tastes shown to significantly differ in intensity. Sucrose, NaCl, monosodium glutamate, lactic acid, and caffeine were mixed in water and adjusted using a 25-1 fractional factorial design (FFD) to reconstruct cheese taste; the optimised construct was compared with a Cheddar cheese to measure similarity for each taste type. The FFD provided knowledge of taste-taste interactions and aided the reconstruction of the taste profile of Cheddar cheese in solution. The reconstructed cheese solution did not significantly differ in overall intensity, saltiness, sourness, umami, and bitterness from the Cheddar cheese based on chi-squared tests. Sweetness was a difficult attribute to adjust due to its relatively low intensity.
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4. |
- Niimi, Jun, et al.
(författare)
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Cross-modal interaction between cheese taste and aroma
- 2014
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Ingår i: International Dairy Journal. - : Elsevier Ltd. - 0958-6946 .- 1879-0143. ; 39:2, s. 222-228
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Tidskriftsartikel (refereegranskat)abstract
- The relative contributions of taste and aroma to the perception of cheese flavour intensity were investigated using a simultaneous gustometer olfactometer. Within a mixture of five tastes and a multicomponent aroma that had a mature Cheddar cheese character, the levels of NaCl, monosodium glutamate (MSG), lactic acid, and aroma were varied according to a 24 full factorial design, all with appropriate controls. NaCl, lactic acid, and aroma significantly contributed to cheese flavour intensity while MSG did not. The contribution of NaCl, lactic acid, and aroma revealed their potential to enhance cheese flavour intensity. These variables were able to compensate each other towards cheese flavour intensity. Changing the levels of taste and aroma together influenced the cheese flavour intensity greater than changing the levels of a single taste or aroma only. Variation in tastant concentration had a larger effect on cheese flavour intensity than did aroma.
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5. |
- Niimi, Jun, et al.
(författare)
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Sensory Interactions between Cheese Aroma and Taste
- 2015
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Ingår i: Journal of sensory studies. - : Blackwell Publishing Ltd. - 0887-8250 .- 1745-459X. ; 30:3, s. 247-257
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Tidskriftsartikel (refereegranskat)abstract
- Cross-modal interactions between cheese aroma and cheese taste, and between aroma were investigated. Aroma was varied by four mixture types and three concentrations, all with a background cheese taste, with four aroma only controls. All cheese aroma samples were produced using 10 aroma compounds commonly found in cheese but at varying individual concentrations of compound. A panel of assessors (n=8) evaluated in triplicate the intensities of four flavor attributes; overall intensity of cheese, fruity cheese, blue cheese, and buttery cheese. The flavor attributes that corresponded to the aroma compound mixtures were perceived highest in intensity; blue cheese flavor (from additional methyl ketones: 2-butanone, 2-heptanone, and 2-nonanone), fruity cheese flavor (from additional esters: ethyl butanoate and ethyl hexanoate) or buttery cheese flavor (from additional diacetyl). An increase in select aroma compound concentrations from the base aroma mixture revealed the effect of aroma mixture interactions on flavor perception. Overall intensity of cheese was significantly suppressed by esters (P<0.01) and diacetyl (P<0.01), while it was unchanged by methyl ketones. Removing taste from the mixture significantly reduced all flavor attribute intensities regardless of cheese aroma character. Changing the aroma character gave different variants of cheese flavor but did not enhance overall intensity of cheese.
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6. |
- Overington, A. R., et al.
(författare)
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Flavour release and perception in cheese bases
- 2010
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Ingår i: Australian Journal of Dairy Technology. - 0004-9433. ; 65:3, s. 162-164
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Tidskriftsartikel (refereegranskat)abstract
- To be able to deliver customised flavours from cheese matrices that have different textures and compositions, it is important to understand how the cheese matrix affects the perception of flavour. It is known that perceived flavours depend not only on the combination of tastants and aroma compounds in a food, but also on the way in which those flavour compounds are released from the food matrix. In addition, flavour perception can be influenced by cross-modal sensory interactions between taste, aroma and texture. In a collaborative research project between Fonterra Research Centre, The University of Otago in New Zealand and CSIRO in Australia, we are evaluating flavour release and cross-modal sensory interactions in a cheese base. Our aim is to understand how perceived flavour is affected by the texture and composition of the cheese and by the combination of tastants and aroma compounds making up the flavour. Sets of cheese bases were created with similar textures but different compositions, and similar compositions but different textures. Flavour compounds were added, and the equilibrium flavour release was studied using proton transfer reaction mass spectrometry, and related to flavour perception using descriptive sensory analysis.
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7. |
- Arrowsmith, CH, et al.
(författare)
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The promise and peril of chemical probes
- 2015
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Ingår i: Nature chemical biology. - : Springer Science and Business Media LLC. - 1552-4469 .- 1552-4450. ; 11:8, s. 536-541
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Tidskriftsartikel (refereegranskat)
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8. |
- Niimi, Jun, et al.
(författare)
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Cross-modal taste and aroma interactions : Cheese flavour perception and changes in flavour character in multicomponent mixtures
- 2016
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Ingår i: Food Quality and Preference. - : Elsevier Ltd. - 0950-3293 .- 1873-6343. ; 48, s. 70-80
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Tidskriftsartikel (refereegranskat)abstract
- The effect of cross-modal sensory interactions between cheese aroma and cheese taste on both cheese flavour intensity and cheese flavour character were investigated. Cheese aroma consisted of a mixture of ten aroma compounds, whilst cheese taste was constructed with the five basic tastes in water solution. Interactions were investigated using a combination of a high resolution design (HRD) and central composite design (CCD). The HRD was 2 aroma×5 NaCl×5 lactic acid levels. The CCD was constructed based on a 23 factorial design that covered the HRD space. Both HRD and CCD gave a total of 57 samples. A panel of experienced assessors (n=8-10) evaluated cheese flavour intensity relative to reference. In addition, samples were sorted based on similarity of flavour character. Fourteen samples were selected for free choice profiling and analysed using generalised procrustes analysis (GPA). Cheese flavour intensity was non-linear and dependent on both taste level and aroma level. The status of flavour character being cheese-like was dependent on taste levels, where either NaCl or lactic acid at a high or low level altered the flavour character to being atypical of cheese. A cheese-like flavour character was maintained across a wide range of NaCl concentrations, but only across a narrow range of lactic acid concentrations. Aroma level changed the character of cheese flavour. Overall, the balance in concentration of both tastes and aroma levels was important in maintaining cheese flavour character as cheese flavour intensity was modified.
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9. |
- Niimi, Jun, et al.
(författare)
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Influence of cross-modal sensory interactions on cheese flavour intensity and character
- 2015
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Ingår i: ACS Symp. Ser.. - Washington, DC : American Chemical Society. - 9780841230699 ; , s. 15-25
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Konferensbidrag (refereegranskat)abstract
- Cross-modal sensory interactions between cheese aroma and taste, and their effect on cheese flavour intensity and difference in flavour character, were determined. NaCl, lactic acid, and aroma were varied at three different levels in combination, according to a 33 full factorial design. The change in cheese flavour intensity and difference in flavour character were measured relative to a reference using a panel (n=9). Model solutions were delivered by the simultaneous gustometer olfactometer. Increasing levels of NaCl, lactic acid, or aroma significantly (p<0.001) enhanced cheese flavour intensity. A significant interaction between NaCl and lactic acid levels with respect to cheese flavour intensity was detected, indicating that cross-modal sensory interactions were not linear. Flavour character also significantly (p<0.001) changed in that raising levels of NaCl decreased the difference in flavour character from the reference, while that of lactic acid or aroma increased the difference
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