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Sökning: WFRF:(Pacek P)

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1.
  • Sajantila, A, et al. (författare)
  • A microsatellite polymorphism in the von Willebrand factor gene : comparison of allele frequencies in different population samples and evaluation for forensic medicine
  • 1994
  • Ingår i: Forensic Science International. - 0379-0738 .- 1872-6283. ; 68:2, s. 91-102
  • Tidskriftsartikel (refereegranskat)abstract
    • The allele frequencies at the tetranucleotide repeat (TCTA) vWA locus in the vWF gene were determined in the general Finnish population, in a population representing an internal isolate of Finland, in the Vologda-Russian population, and in US Black population samples. The allele and genotype frequencies from these population samples were compared with each other and with those reported from Spanish and British population samples. Statistically significant differences were demonstrated between most of the different groups (Finns vs. Vologda-Russians, Finns vs. US Blacks, Finns vs. Spanish, Vologda-Russians vs. US Blacks, Vologda-Russians vs. Spanish, US Blacks vs. Spanish and US Blacks vs. British Caucasians), but not between the two Caucasoid population samples from Finland and Great Britain, nor between or within the subpopulation samples from Finland and those from Vologda-Russia. In addition, the vWA marker was evaluated and demonstrated to be reliable for forensic purposes and paternity testing.
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2.
  • Pacek, P, et al. (författare)
  • Determination of allele frequencies at loci with length polymorphism by quantitative analysis of DNA amplified from pooled samples
  • 1993
  • Ingår i: PCR methods and applications. - : Cold Spring Harbor Laboratory. - 1054-9803. ; 2:4, s. 313-317
  • Tidskriftsartikel (refereegranskat)abstract
    • We present a new method that allows rapid determination of allele frequencies at loci exhibiting length polymorphism. In this method a fluorescence-labeled PCR primer is used to amplify the polymorphic region from pooled DNA samples originating from a large number of individuals. The fluorescent PCR products are separated by gel electrophoresis on an automatic DNA sequencer and the relative amount of the PCR products are determined. The distribution of the PCR products obtained from the alleles present in the pooled samples directly corresponds to the allele frequency in the population in question. The allele frequencies at a short tandem repeat locus in the von Willebrand factor gene and at the D1S80 locus were determined in the Finnish population. We found that the allele frequencies determined by quantitative analysis of PCR products from pooled DNA samples and by analyzing individual samples were in good agreement.
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  • Resultat 1-2 av 2
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tidskriftsartikel (2)
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refereegranskat (2)
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Syvänen, Ann-Christi ... (2)
Sajantila, A (2)
Pacek, P (2)
Peltonen, L (1)
Budowle, B. (1)
Sistonen, P. (1)
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Lukka, M (1)
Nokelainen, P. (1)
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Uppsala universitet (2)
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Engelska (2)

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