| 1. |
- Ingermaa, Fredrika, et al.
(författare)
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Sheep, humans and listeriosis
- 2004
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Ingår i: ISOPOL XV. XV International Symposium on Problems of Listeriosis. Uppsala, Sweden, September 12–15, 2004. - Uppsala : Dep of Food Hygiene, Faculty of Veterinary Medicine. ; , s. Abstract no. 129-
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Konferensbidrag (refereegranskat)
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| 2. |
- Lopez-Valladares, Gloria, 1963-, et al.
(författare)
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Human isolates of Listeria monocytogenes in Sweden during half a century (1958-2010)
- 2014
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Ingår i: Epidemiology and Infection. - 0950-2688 .- 1469-4409. ; 142, s. 2251-2260
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Tidskriftsartikel (refereegranskat)abstract
- Isolates of Listeria monocytogenes (n=932) isolated in Sweden during 1958–2010 from human patients with invasive listeriosis were characterized by serotyping and pulsed-field gel electrophoresis (PFGE) (AscI). Of the 932 isolates, 183 different PFGE types were identified, of which 83 were each represented by only one isolate. In all, 483 serovar 1/2a isolates were distributed over 114 PFGE types; 90 serovar 1/2b isolates gave 32 PFGE types; 21 serovar 1/2c isolates gave nine PFGE types; three serovar 3b isolates gave one PFGE type; and, 335 serovar 4b isolates gave 31 PFGE types. During the 1980s in Sweden, several serovar 4b cases were associated with the consumption of European raw soft cheese. However, as cheese-production hygiene has improved, the number of 4b cases has decreased. Since 1996, serovar 1/2a has been the dominant L. monocytogenes serovar in human listeriosis in Sweden. Therefore, based on current serovars and PFGE types, an association between human cases of listeriosis and the consumption of vacuum-packed gravad and cold-smoked salmon is suggested.
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| 3. |
- Parihar, Vishal Singh, 1979-
(författare)
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Human listeriosis : sources and routes
- 2008
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The bacterium Listeria monocytogenes can cause the disease listeriosis in both humans and animals. For the epidemiological investigation of listeriosis detection and characterisation of the organism are important steps.Paper I. There are few reports on the incidence of L. monocytogenes in clinical samples from humans in India. Therefore, we investigated 144 samples from immunocompromised patients. L. monocytogenes was isolated from two placental bits from women with poor obstetric history, one patient with renal failure and three other patients. Five isolates were positive for the virulence genes hlyA, actA and iap. The sixth isolate was positive for hlyA and actA genes.Paper II. Characterisation of 601 human L. monocytogenes isolates causing invasive listeriosis during the period 1986 to 2007 in Sweden reveals a decrease in serovar 4b strains. Since 1996, serovar 1/2a has become the predominant serovar causing human listeriosis: PFGE analysis revealed two clusters including different serotypes suggesting that we need more studies on genetic relatedness among clinical isolates.Paper III. The incidence of Listeria species in seafood from markets in Goa was studied. One hundred and fifteen raw/fresh seafoods bought at the fish markets were sampled and tested for presence of Listeria spp. L. monocytogenes was detected in 10 samples. L. monocytogenes in raw seafood may pose a health risk in kitchen if contaminating ready-to-eat food.Paper IV. Gravad and cold-smoked salmon are associated with human listeriosis in Sweden. L. monocytogenes was isolated from 11 of 56 products. Serovar 1/2a was predominant, followed by 4b. REA/PFGE typing of the isolates identified five types of L. monocytogenes. One type was identical to a human type, two other were closely related.These findings suggest that gravad and cold-smoked salmon are still possible sources of listeriosis in Sweden.Paper V. Many outbreaks of listeriosis have been related to consumption of dairy-associated products. Therefore, 123 farm bulk milk samples in India and 20 cervico-vaginal samples from dairy cows with reproductive disorders were investigated for L. monocytogenes.L. monocytogenes was isolated from 17.9% of bulk milk samples and from 10% of cervico-vaginal swabs. The virulence gene hlyA was detected in all isolates. These findings represent a public health risk where unpasteurised milk and milk products are largely consumed.Paper VI. Isolates of L. monocytogenes (n=177) from 22 animal species were characterized and compared with human strains isolated between 1986-2006 in Sweden. Although many animals and humans shared pulsovars, they did not appear at the same time or with the same proportion of strains. The pulsovars shared by both animals and humans may indicate that there is an exchange of L. monocytogenes strains between these two groups due to either direct or indirect transmission.
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| 4. |
- Parihar, Vishal Singh, 1979-, et al.
(författare)
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Isolation of Listeria species from farm bulk milk at the receiving dairy plant and cervico-vaginal swabs
- 2007
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Ingår i: Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases. - 0974-0147. ; 28:1/2, s. 53-55
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Tidskriftsartikel (refereegranskat)abstract
- Listeria species were isolated from farm bulk milk (n=123) at the receiving dairy plant and cervico-vaginal samples (n=20) collected from dairy cows with reproductive disorders. Following enrichment and plating on two selective agar media, confirmation of the isolates was based on standard tests. Isolates were subjected to a PCR assay for detection of the hlyA gene. Overall, Listeria spp. were isolated from 30(24.4%) bulk milk samples and four (20%) cervico-vaginal swabs. Listeria monocytogenes was isolated from 17.9%, L. innocua from 1.6%, L. seeligeri from 3.3% and L. welshimeri from 1.6% bulk milk samples. Only L. monocytogenes (10%) and L. innocua (10%) could be isolated from cervico-vaginal swabs. The hlyA gene was detected in all L. monocytogenes isolates. These findings represent a public health risk where homemade unpasteurised milk and milk products are largely consumed.
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| 5. |
- Parihar, Vishal, 1979-, et al.
(författare)
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Zoonotic aspects of Listeria monocytogenes
- 2004
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Ingår i: ISOPOL XV – XV International Symposium on Problems of Listeriosis, Uppsala, Sweden, September 12–15, 2004. - Uppsala. ; , s. Abstract no. 128-
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Konferensbidrag (refereegranskat)
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