| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Holzkämper, Steffen, 1974-, et al.
(författare)
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Late Pleistocene stalagmite growth in Wolkberg Cave, South Africa
- 2009
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Ingår i: Earth and Planetary Science Letters. - Amsterdam : Elsevier. - 0012-821X .- 1385-013X. ; 282, s. 212-221
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Tidskriftsartikel (refereegranskat)abstract
- Little is known about the sequence of climate and environmental change in southern Africa during the last glacial period, in spite of the intimations from records, such as Antarctic ice cores and archaeological sites, that very marked changes took place which would have had profound effects on vegetation and animal distributions across the sub-continent. High-resolution, (semi-) continuous climate and environmental records can be extracted from suitable cave speleothems. Speleothems are reasonably abundant in southern Africa, but their occurrence is patchy in time and space and the records can be difficult to interpret. Here we report our assessment of the stalagmite W5 from Wolkberg Cave in the northeastern part of South Africa, as an archive for glacial-period climatic and environmental shifts. The cave is located at 1450 m asl, in the dolomitic limestones of the Transvaal System in an area currently dominated by C4 grass vegetation. Nine U/Th dates show growth from 58 to 46 ka, and a second brief phase ca. 40 ka, indicating that the available moisture was sufficient to allow speleothems to form. The δ18O and δ13C values along the growth axis show variability in the order of 2‰ for the former, while variability in the latter is characterized by a shift from values near − 2‰ in the older section to + 2‰ or more in the younger part. These high δ13C values are probably the combined result of CO2 degassing of the percolating soil water prior to the carbonate precipitation in the cave chamber, the increasing dominance of C4 over C3 vegetation, and the high percentage of aragonite towards the stalagmite's top. The retrieved data point towards increasingly drier and colder conditions during the growth period of the stalagmite. Furthermore, the high-frequency variations of δ18O values indicate the presence of short term climate oscillations that are probably linked to shifts of the Intertropical Convergence Zone.
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